Linear density analysis of bone repair in rats using digital direct radiograph

Abstract

The objective of this study was to assess bone density measurements for analysis of repairing fractures in rats using direct digital radiography under Dexamethasone effects. Thirty (30) young adult male Wistar rats were used, with an average weight of 200g, which were submitted to general anesthetics by ethyl ether. One rib of each animal was fractured by surgical technique. The rats were divided into 2 groups, named Control group - intraperitoneal administration of saline solution 1 hour prior to operation, and Group 1: intraperitoneal administration of Dexamethasone 0.1mg/Kg of weight 1 hour prior to operation, and two postoperative doses every 12 hours. The animals were sacrificed at 2, 3, 4, 7 and 14 days. The parts containing the hemi-thorax with the fractured rib were removed and submitted to direct digital radiography, where the linear density of the two extremities of the fracture were analyzed by the Digora System. The results showed that in the control group and in Group 1 there was a gradual and significant increase in linear density. The method used made it feasible to supply data that were statistically significant in assessing the gain in bone density during the period of time analyzed. In conclusion, direct digital radiograph is useful for bone density analysis for fracture repairing. The dose of Dexamethasone was not sufficient to alter the gain in bone density at the extremities of fracture.

Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

“…The study by Teixeira et al (2011) also used the radiographic pixel analysis to confirm the presence and intensity of bone resorption in rat’s mandible, observed in a histological section, via histogram tool by establishing pixel values of the ROIs, overcoming a conventional visual analysis. Also, results by Shrout et al, 2003 , Jett et al, 2004 , Bozzo et al, 2004 , and Goes et al (2010) also confirmed the possibility of bone loss detection by analyzing pixel values or density, even if it was not yet visible radiographically, corroborating our results.…”

Biomimetic Metal−Organic Frameworks as Targeted Vehicles to Enhance Osteogenesis

“…[ 38 ] According to Bozzo et al., intraperitoneal administration of DEX 0.1 mg Kg −1 of weight 1 h prior to operation, and two postoperative doses every 12 h repairing fractures in rats. [ 39 ] After calculation, the DEX loading and capacity of ZIF‐8 nanoparticles could meet the requirement for systemic administration of DEX. Furthermore, DEX@ZIF‐8/SCM nanoparticles were mostly locally injected to the defect area, thus, the release content of DEX from nanoparticles could be enough for bone repair.…”

Enhancing Osteoporosis Treatment through Targeted Nanoparticle Delivery of Risedronate: In Vivo Evaluation and Bioavailability Enhancement

“…These animals were then divided into six separate groups, each labelled as A, B, C, D, E and F. Before the commencement of the study, the animals were individually weighed; the average weight was 200 ± 20 g. The initial phase involved the induction of osteoporosis in groups B, C, D, E, and F using Dexona (dexamethasone sodium phosphate) at a dosage of 2 mg/kg body weight (Zydus Alidac, Bangalore, India, 2 mg/mL) via subcutaneous injection once weekly for a duration of 4 weeks [ 23 ]. The subjects were allowed to remain undisturbed for six weeks, during which osteoporosis development was observed [ 24 ]. Group A was designated as the control group, while Group B was designated as the pathogenic control group.…”