Objective:To assess the mesio-distal tooth width in normal, crowded, or spaced dentitions.Materials and Methods:A sample of 192 maxillary and mandibular dental casts of Libyan subjects was selected from a larger cohort. These subjects did not present with craniofacial anomaly, hypodontia, significant attrition, caries, restorations, or history of permanent tooth extraction or orthodontic treatment. The sample was divided into normal, crowded, and spaced groups according to tooth size/arch length discrepancy. Each group included 32 upper and lower dental casts with equal numbers of males (mean (SD) age = 14.7 (1.9) years) and females (mean (SD) age = 15.7 (2.5) years). The mesiodistal (MD) tooth width, sum of the MD tooth widths mesial to the first molars (TTM), sum of the MD width of the four incisors (I), and the sum of the MD width of canine and first and second premolars (CPP) were calculated for each group. The independent Student t-test was applied sequentially to detect significant differences between paired groups. The ANOVA test was undertaken to explore significant differences between the three groups. Pearson coefficient of correlation was used to evaluate the correlation between I and the corresponding CPP in maxillary and mandibular arches.Results:MD tooth width, TTM, I, and CPP were significantly wider in the crowded compared to normal and spaced dentitions (P<0.001), except for the width of the upper left lateral incisors in both normal and crowded groups. Although there was a trend for smaller tooth widths in spaced dentitions compared to normal ones, this was only significant in the maxillary left central incisor, maxillary right and left lateral incisors, maxillary right first premolar, mandibular right lateral incisor, and mandibular right canine (P<0.05). However, the maxillary TTM, I, and CPP in the normal group were significantly greater than in the spaced group (P<0.05). Significant positive correlations existed between the mean values of I and CPP in both the maxillary and mandibular dentitions of all groups (P<0.01).Conclusions:It appears that in the studied Libyan population, the MD tooth width is a significant component of crowding/spacing.