Lineage Tracing and Molecular Real-Time Imaging of Cancer Stem Cells

Jia, Xiaohua; Shen, Guodong; Jia, Jia; Zhang, Yan; Zhang, Dan; Li, Wanjun; 张, 建军; Tian, Jie

doi:10.3390/bios12090703

Cited by 3 publications

(3 citation statements)

References 61 publications

(22 reference statements)

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“…Subsequently, these cells can either be grown in culture or be used in xenograft transplantations. Either way, their single-cell lineages can be monitored to identify whether they can generate differentiated cellular descendants through in vitro or in vivo asymmetric divisions [ 85 , 126 , 127 , 128 ].…”

Section: Cancer Stem Cells (Cscs)mentioning

confidence: 99%

The Concept of Cancer Stem Cells: Elaborating on ALDH1B1 as an Emerging Marker of Cancer Progression

Tsochantaridis

Roupas

Mohlin

et al. 2023

Life

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Cancer is a multifactorial, complex disease exhibiting extraordinary phenotypic plasticity and diversity. One of the greatest challenges in cancer treatment is intratumoral heterogeneity, which obstructs the efficient eradication of the tumor. Tumor heterogeneity is often associated with the presence of cancer stem cells (CSCs), a cancer cell sub-population possessing a panel of stem-like properties, such as a self-renewal ability and multipotency potential. CSCs are associated with enhanced chemoresistance due to the enhanced efflux of chemotherapeutic agents and the existence of powerful antioxidant and DNA damage repair mechanisms. The distinctive characteristics of CSCs make them ideal targets for clinical therapeutic approaches, and the identification of efficient and specific CSCs biomarkers is of utmost importance. Aldehyde dehydrogenases (ALDHs) comprise a wide superfamily of metabolic enzymes that, over the last years, have gained increasing attention due to their association with stem-related features in a wide panel of hematopoietic malignancies and solid cancers. Aldehyde dehydrogenase 1B1 (ALDH1B1) is an isoform that has been characterized as a marker of colon cancer progression, while various studies suggest its importance in additional malignancies. Here, we review the basic concepts related to CSCs and discuss the potential role of ALDH1B1 in cancer development and its contribution to the CSC phenotype.

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Section: Cancer Stem Cells (Cscs)mentioning

confidence: 99%

The Concept of Cancer Stem Cells: Elaborating on ALDH1B1 as an Emerging Marker of Cancer Progression

Tsochantaridis

Roupas

Mohlin

et al. 2023

Life

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“…Lineage tracing is a powerful technique for mapping the dynamic process of cell division, and various strategies to reconstruct cell lineage tracing have been developed [1][2][3][4][5][6][7][8][9][10] . CRISPR-Cas9 genome editing has been integrated into lineage tracing techniques, leveraging its ability to introduce diversity and irreversible mutations at specific genomic loci that can be inherited by subsequent generations, thereby enabling the differentiation of cells within distinct lineage branches [11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Charting Single Cell Lineage Dynamics and Mutation Networks via Homing CRISPR

Wang,

Dong,

Yin

et al. 2024

Preprint

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Single cell lineage tracing, essential for unraveling cellular dynamics in disease evolution is critical for developing targeted therapies. CRISPR-Cas9, known for inducing permanent and cumulative mutations, is a cornerstone in lineage tracing. The novel homing guide RNA (hgRNA) technology enhances this by enabling dynamic retargeting and facilitating ongoing genetic modifications. Charting these mutations, especially through successive hgRNA edits, poses a significant challenge. Our solution, LINEMAP, is a computational framework designed to trace and map these mutations with precision. LINEMAP meticulously discerns mutation alleles at single-cell resolution and maps their complex interrelationships through a mutation evolution network. By utilizing a Markov Process model, we can predict mutation transition probabilities, revealing potential mutational routes and pathways. Our reconstruction algorithm, anchored in the Markov model's attributes, reconstructs cellular lineage pathways, shedding light on the cell's evolutionary journey to the minutiae of single-cell division. Our findings reveal an intricate network of mutation evolution paired with a predictive Markov model, advancing our capability to reconstruct single-cell lineage via hgRNA. This has substantial implications for advancing our understanding of biological mechanisms and propelling medical research forward.

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“…Finally, this Special Issue contains three exceptional review articles that encompass the diverse facets of in vivo imaging [ 4 , 8 , 11 ], from real-time cancer stem cell imaging [ 11 ] to quantitative analysis of human CEST MRI data [ 8 ]. Notably, the comprehensive review by Subasinghe and colleagues (spanning 72 pages (main text), 70 figures, and 400 references) provides an in-depth summary of the plethora of studies focused on hypoxia imaging [ 4 ].…”

mentioning

confidence: 99%