Lineage-independent determination of cell type in the embryonic mouse retina

Turner, David L.; Snyder, Evan Y.; Cepko, Constance L.

doi:10.1016/0896-6273(90)90136-4

Cited by 618 publications

(439 citation statements)

References 33 publications

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“…Since the largest clones observed here did not contain more cells than the clones obtained by retroviral vectors analysis (Turner et al, 1990), this result reinforced our conclusion that the arrays are actual clones that arise from a single cell. Most interestingly, when we compared the proportion of the different cell types generated in the clones with that of previously published retroviral lineage tracing studies (Turner et al, 1990), or with proportions of the different cell types observed in the entire retinal cell population (Turner et al, 1990;Jeon et al, 1998), we found a general similarity both in terms of proportion of cells in the clones (Fig. 7G and Table 1), and as a proportion of clones containing at least one of each cell type ( Fig.…”

Section: Developmental Dynamicssupporting

confidence: 86%

“…Although it is inherently difficult to unambiguously demonstrate that all cell arrays in the IKCre-F line are actual clones, the observation of isolated Cre-recombined RPCs at E10.5, the progressive increase in array size over time, and the finding that the largest arrays observed here do not contain more cells than those observed by retroviral lineage tracing strongly suggest that most, if not all, cell arrays observed in the IKCre-F line are actual clones that derived Developmental Dynamics The current study and Turner et al (1990) measured proportions among randomly labeled clones, whereas numbers from the other studies reflect whole retina analysis.…”

Section: Cre Recombination In Rpcssupporting

confidence: 52%

“…7G and Table 1), and as a proportion of clones containing at least one of each cell type ( Fig. 7H; Turner et al, 1990). These results suggest that the composition of the clonal population in the IKCre-F line is unbiased and generally representative of the global retinal cell population.…”

Section: Developmental Dynamicsmentioning

confidence: 58%

“…Lineage tracing in the mouse retina proved a powerful tool and established the existence of multipotent progenitors able to give rise to all retinal cell types in radially-arranged arrays (Price et al, 1987;Turner et al, 1990). These seminal studies relied on retroviral infection of RPCs in vivo from E12.5, and typically generated clones that widely varied in size.…”

Section: Cre Recombination In Rpcsmentioning

confidence: 99%

“…from a single RPC. Although the classical retroviral lineage studies in the retina generated groundbreaking discoveries and have been extensively cited, conclusions on the potential of early RPCs were based on the analysis of 93 clones generated from virus infection at E12.5 and 222 clones at E13.5 (Turner et al, 1990). Considering the significance of this data for the field, the analysis of a larger number of clones from an earlier time point would be desirable, and in particular mandatory to test various models of retinogenesis.…”

Section: Cre Recombination In Rpcsmentioning

confidence: 99%

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In vivo evidence for unbiased ikaros retinal lineages using an ikaros‐cre mouse line driving clonal recombination

Tarchini

Jolicoeur

Cayouette

2012

Developmental Dynamics

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Background: We showed previously that the transcription factor Ikaros is expressed in early but not late retinal progenitors cells (RPCs), and is necessary and sufficient for the production of early-born neurons. Preliminary evidence using retinal explant cultures qualitatively suggested that Ikaros-positive RPCs might share a common lineage with Ikaros-negative RPCs. Results: To explore further this question in vivo in a quantitative manner, we generated BAC transgenic mouse lines expressing Cre recombinase under the regulatory elements of the Ikaros gene, and crossed them with Cre reporter lines. Different transgenic lines labeled a variable number of RPCs, resulting in either dense or sparse radial arrays of reporter-positive progenies. Analysis of over 800 isolated cell arrays, which are most likely clones, confirmed that Ikaros-expressing RPCs generate both early-and late-born cell types in the same lineage, and that the overall cell composition of the arrays closely resembles that of the population of the mature retina. Interestingly, another sparse line did not label arrays, but appeared to specifically reflect Ikaros postmitotic expression in amacrine and ganglion cells. Key words: lineage; competence; temporal identity; cell fate; cell specification; development Key FindingsThe Ikaros retinal lineage gives rise to both early-and late-born cell types and is confirmed unbiased in vivo One Ikaros-Cre mouse line allows sparse recombination in retinal progenitors and is validated as a tool for in vivo lineage tracing A distinct Ikaros-Cre mouse line allows sparse recombination specifically in some postmitotic early-born retinal cell types

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Section: Developmental Dynamicssupporting

confidence: 86%

Section: Cre Recombination In Rpcssupporting

confidence: 52%

Section: Developmental Dynamicsmentioning

confidence: 58%

Section: Cre Recombination In Rpcsmentioning

confidence: 99%

Section: Cre Recombination In Rpcsmentioning

confidence: 99%

See 3 more Smart Citations

In vivo evidence for unbiased ikaros retinal lineages using an ikaros‐cre mouse line driving clonal recombination

Tarchini

Jolicoeur

Cayouette

2012

Developmental Dynamics

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Retinoblastoma

Nork

Schwartz²,

Doshi³

et al. 1995

Arch Ophthalmol

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Three types of serotonin-containing amacrine cells in tadpole retina have distinct clonal origins

Huang

Moody

1997

J. Comp. Neurol.

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In the Xenopus tadpole there are three different serotonin-containing amacrine cells: large, brightly fluorescent (LB), and small, dimly fluorescent (SD) cells in the inner nuclear layer and displaced (DIS) cells in the ganglion cell layer. To reveal the potential roles of regional cues and lineage factors in their determination, quantitative maps were made of the spatial distribution and blastomere origin of each cell type. LB and SD cells were evenly distributed across the four retinal quadrants, arguing against a hypothesis that these cells are induced differentially by quadrant-specific cues. Blastomere progenitors of the 32-cell embryo are biased to produce only subsets of serotonin amacrine cells: 1) all nine progenitors of one retina produced some SD cells, but only eight produced LB, and only five produced DIS cells; and 2) there are overlapping but distinct subsets of blastomere progenitors for each serotonin subtype. This bias is not simply a reflection of the size of a clone in the retina; significant quantitative differences were observed between the proportion of serotonin progeny and the proportion of the entire retina produced by six of the nine retinal progenitors. This bias also is not simply a reflection of the spatial distribution of a blastomere clone in the retina; the number of LB descendants in each retinal quadrant was statistically different from its progenitor's total contribution to the quadrant. These results indicate that the development of the three different serotonin-containing amacrine cells in the retina is biased by membership in specific blastomere clones.

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Lineage-independent determination of cell type in the embryonic mouse retina

Cited by 618 publications

References 33 publications

In vivo evidence for unbiased ikaros retinal lineages using an ikaros‐cre mouse line driving clonal recombination

In vivo evidence for unbiased ikaros retinal lineages using an ikaros‐cre mouse line driving clonal recombination

Retinoblastoma

Three types of serotonin-containing amacrine cells in tadpole retina have distinct clonal origins

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