Lineage does not regulate the connectivity of projection neurons in the mouse olfactory bulb

Abstract

4Lineage regulates the synaptic connections between neurons in some regions of the invertebrate 5 nervous system. In mammals recent experiments suggest that cell lineage determines the 6 connectivity of pyramidal neurons in the neocortex, but the functional relevance of this 7 phenomenon and whether it occurs in other neuronal types remains controversial. We investigated 8 whether lineage plays a role in the connectivity of mitral and tufted cells, the projection neurons 9 in the mouse olfactory bulb. We used transgenic mice to label neuronal progenitors sparsely and 10 observed that clonally related neurons receive synaptic input from olfactory sensory neurons 11 expressing different olfactory receptors. These results indicate that lineage does not determine the 12 connectivity between olfactory sensory neurons and olfactory bulb projection neurons. 13