Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules

Wilkerson, Melinda J.; Dolce, Karen; Koopman, Timco; Shuman, Wilma; Chun, Ruthanne; Garrett, Laura D.; Barber, Lisa G.; Avery, Anne C.

doi:10.1016/j.vetimm.2005.02.020

Cited by 128 publications

(159 citation statements)

References 38 publications

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“…In the remaining 5 cases no expression of the examined immunoglobulins was found, which may indicate that these lymphomas produce one of the remaining immunoglobulin classes or only immunoglobulin light chains. Similarly, the results of other studies (Teske et al 1994, Fournel-Fleury et al 1997, Wilkerson et al 2005 indicate that tumors expressing IgM are predominant among canine B-cell lymphomas, representing from 40% to about 70% of cases. Tumors expressing other immunoglobulin classes are much rarer, they constitute 10-20% of examined cases (Teske et al 1994, Fournel-Fleury et al 1997, Wilkerson et al 2005.…”

Section: Discussionsupporting

confidence: 68%

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

Sokołowska¹,

Micun²,

Zabielska³

et al. 2010

Polish Journal of Veterinary Sciences

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Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of λ light chains and κ light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (sκ/sλ). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (cκ/cλ). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM + and IgG + phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin λ light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.

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Section: Discussionsupporting

confidence: 68%

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

Sokołowska¹,

Micun²,

Zabielska³

et al. 2010

Polish Journal of Veterinary Sciences

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“…13 Similarly, a 2008 study 6 also reported a case of lymphoma with the CD3+ CD21+ phenotype, but a similar finding was not reported in other studies of dogs with lymphoid neoplasia. 1,14 In cases of canine lymphoma reported previously, 13 the coexpression of CD3 and CD21 had been shown in nodal lymphocytes from 4 of 59 dogs. Among these 4 cases, 1 had a TCRc clonal gene rearrangement and 2 had an IgH clonal gene rearrangement.…”

Section: Discussionmentioning

confidence: 68%

“…4 More recently, a similar assay was developed based on the canine genome database by targeting the T-cell receptor gamma chain (TCRc) gene 15 and the immunoglobulin heavy chain (IgH) gene. 9 Although the diagnostic value of PCR analysis has been demonstrated in several types of lymphoproliferative disorders, 7,[11][12][13][14] only limited studies have been done to identify neoplastic proliferation of lymphocytes in dogs with small-cell lymphocytosis. In the present study, immunophenotypical evaluation was performed on the blood of dogs with small lymphocytosis.…”

Section: Introductionmentioning

confidence: 99%

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

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Abstract. Lymphocytosis caused by neoplastic proliferation of small lymphocytes is occasionally difficult to distinguish by morphological examination from nonneoplastic lymphocytosis. To examine the clinical utility of gene rearrangement analysis for demonstrating neoplastic proliferation of small lymphocytes, gene rearrangement analysis was performed in comparison with immunophenotyping using peripheral lymphocytes in dogs with small lymphocytosis. Thirty-one dogs with small-cell lymphocytosis (8,100-884,300/ml) were enrolled. By immunophenotyping, lymphocytosis of all dogs was suggested to be neoplastic in nature based on the detection of marked expansion of phenotypically homogeneous lymphocytes or the presence of an aberrant antigen-expressing population of lymphocytes. In contrast, gene rearrangement analysis represented clonality in 27 dogs (detection rate of 87%). From the present study, gene rearrangement analysis was considered to be worthwhile to strengthen the evidence of neoplastic proliferation of small lymphocytes when coupled with immunophenotyping and to be a suitable diagnostic substitute if immunophenotyping is not available in clinical practice.

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“…20,23,24 These findings were in contrast to human AIL, where most cases have been classified as Tcell-rich B-cell lymphomas. 14 The present study did not investigate B-and T-cell clonality, and clonality studies would be necessary to more fully characterize feline AIL.…”

mentioning

confidence: 89%

Metastatic Angioinvasive Lymphoma (Lymphomatoid Granulomatosis) in a Cat

Rogers¹,

Aliano

2009

J VET Diagn Invest

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Abstract. A subcutaneous mass removed from the right rear leg of a 17-year-old, spayed, female Domestic Shorthair cat was characterized histopathologically by granulomatous inflammation, sheets of large atypical lymphoid cells, and necrosis. The walls of the small and medium caliber blood vessels were invaded transmurally by atypical lymphoid cells. A diagnosis of angioinvasive lymphoma (AIL), or lymphomatoid granulomatosis, was made based on histopathologic findings. The cat was euthanized 2 months later because of recurrence of the mass and elevated serum alanine aminotransferase activity, and a necropsy was performed. The histopathologic lesion of AIL was seen in the skin and subcutis of the right rear leg, and neoplastic cell infiltrates were seen in adjacent skeletal muscle, right superficial inguinal lymph node, liver, and spleen. By immunohistochemistry, variable numbers of neoplastic cells expressed B-lymphocyte antigen 36 (BLA36) or cluster of differentiation (CD)3 markers, indicative of B-and T-cell lineages, respectively. Neoplastic cells were uniformly positive for vimentin and uniformly negative for cytokeratins and myeloid/histiocytic antigen. Although the cat had received a Rabies virus vaccine subcutaneously in the right rear leg 6 months earlier, the AIL lesion was not typical of vaccine-induced sarcomas. The AIL in this cat was unusual because the extrapulmonary metastases involved the skin and subcutis.

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Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules

Cited by 128 publications

References 38 publications

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Metastatic Angioinvasive Lymphoma (Lymphomatoid Granulomatosis) in a Cat

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