Lineage analysis of human papillomavirus type 18 based on E6 region in cervical samples of Iranian women

Salavatiha, Zahra; Shoja, Zabihollah; Heydari, Nasrin; Marashi, Sayed Mahdid; Younesi, Sarang; Nozarian, Zohreh; Jalilvand, Somayeh

doi:10.1002/jmv.26283

Cited by 13 publications

(18 citation statements)

References 36 publications

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“…Although the sample size was small for HPV 18, A3 was the predominant variant, followed by A4. This finding is inconsistent with the results of a study by Salavatiha et al, which reported HPV 18 A4 variant to be predominant in Iran, [ 23 ]. The HPV 18 A1 variants are prevalent in the Asia-Pacific region, and A3 variants account for a large proportion of variants in Europe, Southern/Central Asia, and America [ 7 ].…”

Section: Discussioncontrasting

confidence: 99%

Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women

Kesheh

Barazandeh

Kaffashi

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Human papillomavirus (HPV) 16 and HPV 18 account for 75% of all cervical cancers. The L1 gene, encoding the major surface protein (MSP), is used to classify HPV types (lineages and sublineages), genotypes, and intratypic variants. Therefore, this study aimed to investigate the lineages, sublineages, genetic variabilities, and mutation effects on transcription factor binding sites by using partial sequences of the HPV 16 and HPV 18 long control regions (LCRs) in these samples. Materials and Methods. After DNA isolation from 56 positive samples, the LCR of HPV 16 and HPV 18 were amplified using specific primers, and phylogenetic trees were drawn through MEGA X. Compared to the reference sequences, single nucleotide polymorphisms (SNPs) were identified. The transcription binding sites were also evaluated using the online PROMO database. Results. The LCRs of 52 samples were successfully sequenced. Overall, 81.58% of all HPV 16 variants belonged to the D1 sublineage, followed by A4 (13.16%), A1 (2.63%), and C1 (2.63%) sublineages. All HPV 18 isolates belonged to A sublineage, 92.85% to A3 sublineage, and 7.15% to A4 sublineage. Out of 27 SNPs in the HPV 16 LCR, A7382T, T7384G, C7387T, C7393G, A7431G, T7448C, and C7783A were HPV 16-specific. Also, among 14 SNPs in the HPV 18 LCR, C7577A and A7943T were not previously reported. An insertion (C) between 7432 and 7433 positions was identified in all studied HPV 16 variants. Besides, most of the HPV 16 mutations were embedded in the YY1, TFIID, Oct-2, and NF-1 binding sites, while c-Fos and MBF1, as the most common binding sites, were affected by HPV 18 LCR mutations. Conclusion. The present results showed that D1 and A3 were the dominant sublineages of HPV 16 and HPV 18, respectively. Therefore, women infected with these variants need to be examined in further longitudinal studies to obtain more information about the oncogenic potential of these dominant variants in Iran. Besides, YY1, TFIID, Oct-2, NF-1, c-Fos, and MBF1 were the most frequent binding sites, which were influenced by the mutations.

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Section: Discussioncontrasting

confidence: 99%

Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women

Kesheh

Barazandeh

Kaffashi

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…There are few studies that characterized the common HPV 16, 18, 31, and 45 lineages/sublineages in Iran. [13][14][15] The PCR products were subjected to sequencing using bidirectional direct sequencing as described above. All sequences of this study were deposited at http://www.ncbi.nlm.nih.gov/.…”

Section: Introductionmentioning

confidence: 99%

“…Although the geographical distribution of HPV types in Iran is identified, there is less data with regard to HPV lineages or sublineages. There are few studies that characterized the common HPV 16, 18, 31, and 45 lineages/sublineages in Iran 13–15 . Therefore, in this study, basic data was provided regarding HPV 56 lineages and sublineages circulating among Iranian women.…”

Section: Introductionmentioning

confidence: 99%

“…There are few studies that characterized the common HPV 16, 18, 31, and 45 lineages/sublineages in Iran. [13][14][15] Therefore, in this study, basic data was provided regarding HPV 56 lineages and sublineages circulating among Iranian women. The complete E6 gene of HPV 56 (nucleotide [nt] 102-566) was evaluated to find genetic variability.…”

Section: Introductionmentioning

confidence: 99%

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Lineage and sublineage analysis of human papillomavirus type 56 in cervical samples of Iranian women

Jalali-Alhosseini

Shoja

Soleimani‐Jelodar

et al. 2021

Journal of Medical Virology

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Understanding the regional lineages and sublineages of human papillomavirus type 56 (HPV 56) would be of great importance for further evolutionary, epidemiological, and biological investigations. To identify the distribution of lineages and sublineages of HPV 56 in Iran, the sequence variations of the E6 gene were analyzed in normal, premalignant, and malignant samples obtained from the cervix. In total, 58 HPV 56positive samples were investigated by nested-PCR and followed by bidirectional direct nucleotide sequencing analysis. Both lineages A and B were identified in the studied samples. Lineage B was dominant as it was detected in 88.4% of all samples and the remaining samples belonged to lineage A (11.6%). Sublineages A1 and A2 were detected in 3.3% and 8.3% of all samples, respectively. With regard to the pathological stages of cervical specimens, no statistically significant differences were found in the three studied groups (p > 0.05). In conclusion, our findings showed that lineage B of HPV 56 was prevalent in Iran. However, further studies with a larger sample size are warranted to estimate the pathogenicity risk of HPV 56 lineages/ sublineages to the progression of cervical cancer among Iranian women.

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“…Whereas the spreading of HPV types is identified in Iran, there is much less known about HPV variants. Previous studies were investigated the common HPV 16, 18, 31, and 45 variants in Iran [19][20][21]. Regard to above-mentioned data that HPV 39 is one of the five most common HPV types in Iranian women with cervical cancer [14], this study aimed to investigate the frequent HPV 39 variants circulating in Iran.…”

Section: Introductionmentioning

confidence: 99%

Lineage analysis of human papillomavirus type 39 in cervical samples of Iranian women

Hosseini

Shoja

Arashkia

et al. 2021

Virol J

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Background The data with regards to the regional variants of distinct HPV types is of great value. Accordance with this, this study aimed to investigate the sequence variations of E6 gene and long control region of HPV 39 among normal, premalignant and malignant cervical samples in order to characterize the frequent HPV 39 variants circulating in Tehran, Iran. Methods In total, 70 cervical samples (45 normal, 16 premalignant, and 9 malignant samples) infected with HPV 39 were analyzed by nested-PCR and sequencing. Results Our results revealed that all samples belonged to A lineage. Almost all sequences (98.6%) were classified in A1 sublineage and only one sample (1.4%) was A2 sub lineage. Conclusions Our findings showed that lineages A, sublineage A1, is dominant in Tehran, Iran. However, the small sample size was the most important limitations of this study. Further studies with larger sample size from different geographical regions of Iran are necessary to estimate the pathogenicity risk of HPV 39 variants in this population.

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Lineage analysis of human papillomavirus type 18 based on E6 region in cervical samples of Iranian women

Cited by 13 publications

References 36 publications

Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women

Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women

Lineage and sublineage analysis of human papillomavirus type 56 in cervical samples of Iranian women

Lineage analysis of human papillomavirus type 39 in cervical samples of Iranian women

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