Lindnera saturnus Kullanılarak Hücresel Biyodönüşüm Yolu ile Fuzel Yağından Muz Aroması Eldesi

Abstract

An efficient biotransformation of isoamyl alcohol obtained from fusel oil to isoamyl acetate in a molasses based medium via in situ product removal (ISPR) with macroporous adsorption resin was carried out with Lindnera saturnus. Nine types of macroporous adsorption resins with different polarities and surface areas were tested with synthetic medium and then in batch and fed-batch cultivations. H103 resin had the best adsorption capacity because of its large and nonpolar surface areas. The isoamyl acetate concentration was increased with 42 and 30 folds in batch and fed-batch cultivations, respectively, compared to the biotransformations without addition of adsorbent resin. When 1 g H103 resin (wet w/v) was added to 50 mL of the biotransformation medium, the total isoamyl acetate concentration achieved was 1.9 g/L, of which 123 mg/L remained in the aqueous phase and 1787 mg/L was adsorbed onto the resin, within 120 h in fed-batch system. This was the highest isoamyl acetate yield by biotransformation until now and was remarkable for making the process more feasible for industrial application.