Lindane degradation by cell-free extracts of Clostridium rectum

Ohisa, Naganori; Yamaguchi, Masuro; Kurihara, Norio

doi:10.1007/bf00446880

Cited by 101 publications

(51 citation statements)

References 15 publications

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“…Chlorobenzene is then produced by a dehydrochlorination reaction. Several reports confirmed the presence of ␥-TCCH as an intermediate in the anaerobic degradation of ␥-HCH (11,58,64,126). The degradation of ␥-HCH also results in the formation of small amounts of trichlorinated benzenes and benzene itself (64).…”

Section: Anaerobic Degradationmentioning

confidence: 85%

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Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

349

338

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SUMMARY Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation.

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Section: Anaerobic Degradationmentioning

confidence: 85%

“…3) (11,21,98,126,137). Ohisa et al (126) proposed that the degradation proceeds through two dichloroeliminations, resulting in the formation of ␥-3,4,5,6-tetrachloro-1-cyclohexene (␥-TCCH) and, subsequently, 5,6-dichlorocyclohexa-1,2-diene. Chlorobenzene is then produced by a dehydrochlorination reaction.…”

Section: Anaerobic Degradationmentioning

confidence: 99%

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

349

338

View full text Add to dashboard Cite

show abstract

“…Biodegradation of HCH isomers has been studied in soil [5], soil slurry [6] etc. A lot of information is available on the biodegradation of γ-HCH and other isomers of HCH using pure bacterial cultures, Pseudomonas aeruginosa, ITRC-5, Xanthomonas species, Clostridium rectum, Pandoraea spp etc [7][8][9][10]. Studies have also been carried out on the degradation of HCH isomers using sewage sludge under aerobic and anaerobic conditions [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Mohapatra¹

2015

J Bioremed Biodeg

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Hexachlorocyclohexane (HCH) isomers are reported to persist in the environment long after their usage is discontinued. Pleurotus florida, a white rot fungus, was found to degrade not only γ-HCH but also its more persistent isomers individually as well as a mixture in a Raper's complete medium. Within 30 days γ-HCH degraded completely, whereas other 3 isomers degraded 92-99%. The degradation rate was γ-HCH>β-HCH>α-and δ-HCH. The mycelium biomass was free from γ-HCH residues but accumulated about 3% residues of other 3 isomers. Presence of intermediate metabolites was not detected indicating complete mineralization of HCH isomers. Ability of P. florida to degrade HCH isomers was further studied in soil by amendment with spent mushroom substrate (SMS). SMS addition could marginally increase degradation of α-, β-and δ-HCH, but significantly increased degradation of γ-HCH. When the study was repeated similar trend was observed. The half-life of degradation of γ-HCH was 439-570 days in un-amended soil while 37-42 days in SMS amended soil. For other 3 stable isomers the half-life was reduced from 686-828 to 88-125 days by SMS amendments. These results indicate that SMS from P. florida cultivation can be utilized for bioremediation of HCH contaminated site.

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“…Anaerobic degraders such as Clostridium spp. have been isolated (26,34), and their degradative pathways have been well characterized (11,12,20,35). Heritage and MacRae (11,12) have used a washed cell suspension (11) and a cell extract (12) to show that -y-HCH is degraded via y-3,4,5,6-tetrachlorocyclohexene (.y-TeCCH) by Clostridium sp.…”

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confidence: 99%

Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane

et al. 1991

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Pseudomonas paucimobilis UT26 is capable of growing on 'y-hexachlorocyclohexane (,y-HCH). A genomic library of P. paucimobilis UT26 was constructed in Pseudomonas putida by using the broad-host-range cosmid vector pKS13. After 2,300 clones were screened by gas chromatography, 3 clones showing y-HCH degradation were detected. A 5-kb fragment from one of the cosmid clones was subcloned into pUC118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a fragment of ca. 500 bp was responsible for the conversion of y-HCH to 1,2,4-trichlorobenzene via -y-pentachlorocyclohexene. Nucleotide sequence analysis revealed an open reading frame (linA) of 465 bp within the fragment. The nucleotide sequence of the linA gene and the deduced amino acid sequence showed no similarity to any known sequences.

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Lindane degradation by cell-free extracts of Clostridium rectum

Cited by 101 publications

References 15 publications

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane

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