lincRNA‑p21 inhibits the progression of non‑small cell lung cancer via targeting miR‑17‑5p

Abstract

Non-small-cell lung cancer (NSCLC) is well established as one of the major subtypes of human lung cancer. NSCLC is characterized by a high incidence rate and poor patient prognosis. Previous studies have identified that long intergenic non-coding RNA (lincRNA) serves a key role in the development of tumor and malignant metastasis. However, the majority of the underlying mechanisms for lincRNA deregulation in various diseases, including cancer and diabetes, have not been completely elucidated. In the present study, the deregulation of lincRNA-p21 in NSCLC tumor tissues in comparison to adjacent healthy tissues was examined using reverse transcription-quantitative polymerase chain reaction. Furthermore, the effect of lincRNA-p21 overexpression and knockdown on different NSCLC cell lines was further investigated in vitro. The association between lincRNA-p21 expression and microRNA (miR)-17-5p level in NSCLC tumor cells was also investigated to clarify the underlying mechanism. The influence of miR-17-5p on different NSCLC cell lines A549 and PC9 were also examined in vitro using miR-17-5p mimics and inhibitors. Bioinformatics and luciferase assays were conducted to verify the direct binding sites on lincRNA-p21 for miR-17-5p. The results demonstrated that there was a significant low-expression of lincRNA-p21 in NSCLC tumor tissues, and lincRNA-p21 effectively inhibited the progression of lung cancer cells by suppressing cell proliferation and migration and promoting cell apoptosis. An evident negative association between lincRNA-p21 and miR-17-5p expression was observed, and the inhibitory effect of overexpressed lincRNA-p21 on lung cancer cells was counteracted by miR-17-5p. Bioinformatics and luciferase reporter analysis results confirmed that miR-17-5p is a direct target for lincRNA-p21. The present study provides evidence for lincRNA-p21 to inhibit the progression of NSCLC via direct targeting of a miR-17-5p associated signaling pathway.

Data Mining and Expression Analysis of Differential lncRNA ADAMTS9-AS1 in Prostate Cancer

“…Recently, many studies have supported a novel regulatory mechanism of lncRNA in human cancers, mainly represented by the interaction between lncRNA and miRNA. LncRNA can act as a ceRNA or molecular sponge to regulate miRNA expression and regulate the occurrence and progression of many cancers (Tu et al, 2019;Ao et al, 2019;Yu et al, 2019). For example, lncRNA Unigene 56159 promotes the epithelial-mesenchymal transition of hepatoma cells by acting as a ceRNA of miR-140-5p (Lv et al, 2016).…”

ADAMTS9-AS1 Constrains Breast Cancer Cell Invasion and Proliferation via Sequestering miR-301b-3p

“…To date, lncRNA/miRNA axis has been a limelight in the field of cancer. LncRNA can trap miRNA, regulate mRNA, and modulate progression of various cancers ( Ao et al, 2019 ; Tu et al, 2019 ). This paper verified miR-301b-3p/ADAMTS9-AS1/TGFBR2 interaction by bioinformatics method, RIP and dual luciferase assay.…”

Long non‐coding RNA CASC19 facilitates non‐small cell lung cancer cell proliferation and metastasis by targeting the miR‐301b‐3p/LDLR axis

“…Abundant investigations have revealed that lncRNAs regulate tumor progression via regulation of miRNAs 23–25 . Interestingly, the present study revealed that miR‐130b‐3p and miR‐301b‐3p was related to CASC19 and was up‐regulated under CASC19 knockdown.…”