LINC01433 promotes hepatocellular carcinoma progression via modulating the miR‐1301/STAT3 axis

Abstract

Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles in hepatocellular carcinoma (HCC) tumor progression. LINC01433 has been implicated in the progression of lung cancer. However, its biological role in HCC remains poorly understood. In our current study, we focused on the detailed mechanism of LINC01433 in HCC development. First, it was exhibited that LINC01433 was remarkably elevated in HCC cells, which indicated that LINC01433 was involved in HCC. Then, knockdown of LINC01433 was able to restrain HCC cell proliferation and cell colony formation and greatly induced cell apoptosis. On the contrary, overexpression of LINC01433 promoted HCC cell proliferation, increased cell colony formation, and enhanced cell invasion capacity. Subsequently, we found that miR-1301 was remarkably decreased in HCC cells, and it can serve as a target of LINC01433 according to bioinformatics analysis. In addition, the binding correlation between them was validated by performing RNA pull-down experiments and RIP assay. Moreover, STAT3 was predicted and validated as a target of miR-1301, and it was shown that miR-1301 mimics significantly suppressed STAT3 in HCC cells.Finally, in vivo models were established, and the results demonstrated that silencing of LINC01433 could repress HCC development through modulating miR-1301 and STAT3. Taken together, these results indicated in our study that LINC01433 participated in HCC progression through modulating the miR-1301/STAT3 axis and it might act as a novel biomarker in HCC diagnosis and treatment.

Long noncoding RNA FAM83A-AS1 facilitates hepatocellular carcinoma progression by binding with NOP58 to enhance the mRNA stability of FAM83A

“…LINC01287/miR-298/STAT3 feedback loop modulates cell growth and the epithelial-to-mesenchymal transition in HCC [20]. LINC01433 facilitates the development of HCC by regulating the miR-1301/STAT3 axis [21]. Nevertheless, the role of FAM83A-AS1 in HCC is largely unknown.…”

<p>lncRNA SLCO4A1-AS1 promotes growth and invasion of bladder cancer through sponging miR-335-5p to upregulate OCT4</p>

“…Cell migration and invasion were measured using a 24-well Transwell system (8 µm pore; Costar, Badhoevedorp, the Netherlands) by using Transwell assay based on a previous study. 9 rna isolation and quantitative reverse transcriptase Pcr (qrT-Pcr)…”

“…An increasing amount of studies have demonstrated that lncRNAs play vital functions in various biological processes and are closely correlated with human diseases, including cancer 7. By participating in the regulation of cell proliferation, migration, invasion, and differentiation, lncRNAs could affect or suppress tumorigenesis 8,9. For example, lncRNA DUXAP8 promotes the progression of renal cell cancer 10.…”

“…7 By participating in the regulation of cell proliferation, migration, invasion, and differentiation, lncRNAs could affect or suppress tumorigenesis. 8,9 For example, lncRNA DUXAP8 promotes the progression of renal cell cancer. 10 LINC00152 enhances the proliferation and metastasis of oral squamous cell cancer.…”

MiR-1301-3p Inhibits Epithelial to Mesenchymal Transition via Targeting RhoA in Pancreatic Cancer

“…Similarly, NNT-AS1 promoted bladder cancer cell proliferation by targeting miR-1301-3p/PODXL axis and activating Wnt pathway [13] . Besides, ABHD11-AS1 and LINC01433 upregulated STAT3 level by sponging miR-1301-3p and promoted tumor progression in papillary thyroid carcinoma and hepatocellular carcinoma, respectively [14,15] . Hence, the function of miR-1301-3p is needed to elucidate for pancreatic cancer.…”