LINC01291 promotes hepatocellular carcinoma development by targeting the miR‐186‐5p/OXSR1 axis

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Recent studies have demonstrated that lncRNAs play an important role in tumorigenesis. LINC01291 has been confirmed to be involved in the proliferation and migration of different cancers, although the function of LINC01291 in HCC is still unknown.Methods: First, the expression of LINC01291 in 50 paired HCC tissues, adjacent normal tissues and HCC cell lines was measured by a quantitative real-time polymerase chain reaction. Then, the function of LINC01291 in HCC cell proliferation, migration and invasion was measured by colony formation, Cell Counting Kit-8 assays, wound healing assays and transwell assays. In addition, E-cadherin, N-cadherin, vimentin and oxidative stress-responsive 1 (OXSR1) protein expression levels were assessed via western blotting. Luciferase reporter assays were used to confirm the relationship between LINC01291 and miR-186-5p, as well as miR-186-5p and OXSR1 mRNA.Rescue assays and in vivo experiments further confirmed the LINC01291/miR-186-5p/OXSR1 axis in the progression of HCC.Results: LINC01291 was upregulated in both HCC tissues and cell lines. Knockdown of LINC01291 inhibited the proliferation, migration, invasion and epithelialmesenchymal progression (EMT) of HCC cells. In addition, LINC01291 could overexpress OXSR1 by sponging miR-186-5p, and OXSR1 overexpression or miR-186-5p inhibition could rescue the effect of LINC01291 knockdown in YY-8103 cell lines. In addition, lentiviral sh-LINC01291 could effectively inhibit the growth of subcutaneous YY-8103 xenograft tumors, whereas the anticancer effect could be reversed by cotransfection with in-miR-186-5p or ov-OXSR1.Conclusions: LINC01291 can promote the proliferation, migration, invasion and EMT of HCC cells via the miR-186-5p/OXSR1 axis, and sh-LINC01291 can inhibit tumor growth in a xenograft mouse model. Highlights• LINC01291 is upregulated in HCC by database and a qRT-PCR.• LINC01291 can promote the development of HCC by regulating the miR-186-5p/OXSR1 axis.Jian Chu, Guangyong Geng and Xiaoming Ai contributed equally to this work.

CircNRIP1 KNOCKDOWN ALLEVIATES LIPOPOLYSACCHARIDE-INDUCED HUMAN KIDNEY 2 CELL APOPTOSIS AND INFLAMMATION THROUGH miR-339-5p/OXSR1 PATHWAY

“…OXSR1 can combine with miR-25-3p to inhibit epileptiform discharges (31). OXSR1 contributes to hepatocellular carcinoma cell proliferation and motility by interacting with miR-186-5p as well as LINC01291 (32). Another study showed that OXSR1 expression was associated with vascular dysfunction and hypertension in autosomal-dominant polycystic kidney disease (33).…”

Hsa_circ_0005085 may suppress cutaneous squamous cell carcinoma growth and metastasis through targeting the miR‐186‐5p/LAMC1 axis

“…Recent research has indicated that the abnormal expression of miR-186-5p might partake in the regulation of malignant behaviors in different human cancers. 35,36 Of interest, related literature suggested that miR-186 might serve as a carcinogenic factor by promoting cell growth in CSCC. 20,37 In accordance with these previous studies, our data exhibited that miR-186-5p could be highly expressed in tumor tissues and cells.…”

“…The RIP experiment was further verified, and it was found that miR‐186‐5p could bind with hsa_circ_0005085 in a targeted manner. Recent research has indicated that the abnormal expression of miR‐186‐5p might partake in the regulation of malignant behaviors in different human cancers 35,36 . Of interest, related literature suggested that miR‐186 might serve as a carcinogenic factor by promoting cell growth in CSCC 20,37 .…”