LINC01006 facilitates cell proliferation, migration and invasion in prostate cancer through targeting miR-34a-5p to up-regulate DAAM1

Abstract

Background Prostate cancer (PCa) is a kind of malignancy occurring in the prostate gland. Substantial researches have proved the major role of long noncoding RNAs (lncRNAs) in PCa. However, the role of long intergenic non-protein coding RNA 1006 (LINC01006) in PCa has not been investigated yet. Methods RT-qPCR was used to examine the expression levels of LINC01006 and its downstream targets. The function of LINC01006 in PCa was tested by in vitro and in vivo assays. With application of RNA pull down, RNA immunoprecipitation (RIP) and luciferase reporter assays, the interaction among LINC01006, miR-34a-5p and disheveled associated activator of morphogenesis 1 (DAAM1) were verified. Results LINC01006 expression presented high in PCa cell lines. LINC01006 silencing suppressed cell proliferative, migratory, invasive capacities while accelerated apoptotic rate. Besides, LINC01006 knockdown also suppressed tumor growth and metastasis in vivo. Furthermore, miR-34a-5p, a tumor suppressor in PCa, was sponged by LINC01006. Moreover, DAAM1 was targeted by miR-34a-5p and promoted PCa progression. More intriguingly, rescue assays suggested that the inhibitory effect of LINC01006 knockdown on PCa development was offset by DAAM1 overexpression. Conclusions LINC01006 promoted PCa progression by sponging miR-34a-5p to up-regulate DAAM1, providing a novel target for PCa therapy.

Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

“…For example, a study has manifested that miR-34a is a possible onco-suppressor for HCC [ 14 ]. In the meantime, miR-34a also could be regarded as a suppressor of lots of cancers including HCC, ovarian cancer and prostate cancer [ 15 – 17 ]. A study has revealed that up-regulated miR-34 in hepatic stellate cells relieves the progression of liver fibrosis [ 18 ].…”

LINC01006 and miR-3199 Serve as Novel Markers of Poor Prognosis in Colon Cancer and Regulate Cell Proliferation, Migration and Invasion

“…In pancreatic cancer and prostate cancer, LINC01006 promotes cell growth and metastasis, providing a novel target for cancer therapy. 17 , 18 A recent study about cervical cancer showed LINC01006 downregulation not only inhibited the proliferation and metastasis of cervical cancer cells but also promoted cell apoptosis. 17 In this study, LINC01006 exhibits tumor‑promoting functions in colon cancer and provides the basis for identifying LINC01006 as targeted therapy.…”

Long non‑coding RNA LINC01006 exhibits oncogenic properties in cervical cancer by functioning as a molecular sponge for microRNA‑28‑5p and increasing PAK2 expression

“…LINC01006 is upregulated in prostate ( 20 ) and pancreatic ( 21 ) cancers and plays pro-oncogenic roles. By contrast, this lncRNA is weakly expressed in gastric cancer and exhibits a notable association with age, tumor location, tumor size and venous invasion ( 22 ).…”

“…Previous studies have confirmed the abnormal expression of the long intergenic non-protein-coding RNA 1006 (LINC01006) in prostate ( 20 ), pancreatic ( 21 ) and gastric ( 22 ) cancers. However, whether LINC01006 plays important roles in cervical cancer remains unclear.…”