LINC00961 inhibits the migration and invasion of colon cancer cells by sponging miR‐223‐3p and targeting SOX11

H, Wu; Dai, Yawei; Zhang, Dexiang; Zhang, Xiaoyu; He, Zhaohong; Xie, Xiaojun; Cai, Chudong

doi:10.1002/cam4.2850

Cited by 18 publications

(9 citation statements)

References 30 publications

(42 reference statements)

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“…25 LINC00961, as a tumor suppressor gene, up-regulates the expression of its target gene SOX11 by acting as a miR-223-3p sponge to inhibit the migration and invasion of colorectal cancer. 26 Compared with normal colonic mucosa, miR-200a-5p was significantly up-regulated in colorectal cancer, but colon adenoma is not substantially different from normal mucosa. 27,28 It was reported that miR-200a-5p and its target gene RNF11 were differentially expressed in cardiac tissue, and overexpressed miR-200a-5p induces RIP3-dependent necroptosis.…”

Section: Discussionmentioning

confidence: 92%

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

Yang¹,

Lü²,

Wang³

et al. 2022

IJGM

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This study aims at exploring the relationship between necroptosis-related miRNAs and colon cancer prognosis. Methods: We downloaded the miRNA sequencing data from the TCGA, and eight differentially expressed necroptosis-related miRNAs were screened. Then, we used Cox regression analysis to establish a prediction model of necroptosis-related miRNA. Finally, the prognosis related miRNAs were used to predict the target genes, and functional analysis was used to explore the potential mechanism of these target genes. Results:The miRNA-seq data of 444 COAD cases were downloaded from TCGA. We identified 8 differentially expressed miRNAs (has-miR-16-5p, has-miR-141-3p, has-miR -148a-3p, has-miR-425-5p, has-miR-7-5p, has-miR-223-3p, has-miR-200a-5p, and has-miR -500a-3p), then Cox analysis was performed for determining eight-miRNA signature prognostic biomarkers with obviously different OS. The area under the curve (AUC) of receiver operating characteristic (ROC) curve for predicting 1-, 3-, and 5-year survival were 0.663, 0.653 and 0.639, respectively. The multivariate analysis also implied that the risk score was an independent prognostic factor considering other confounding factors (HR = 1.847, 95% CI = 1.197-2.848, P = 0.006). According to the Kaplan-Meier analysis, the expression of hsa-miR-500a-3p (P = 0.003), hsa-miR-16-5p (P = 0.004) and hsa-miR-148a-3p (P = 0.035) significantly affected OS outcomes. We predicted the target genes of these three miRNAs and then screened 10 hub genes (CCND1, SMAD3, SMAD2, CDK1, TGFB2, CDC25A, CHEK1, VEGFA, CCNE1, WEE1). In addition, CHEK1 was associated with the survival prognosis. Conclusion: Our study demonstrated that necroptosis is closely associated with colon cancer, and the model of eight necroptosis-related miRNAs are potentially useful prognostic biomarkers and therapeutic targets for colon cancer.

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Section: Discussionmentioning

confidence: 92%

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

Yang¹,

Lü²,

Wang³

et al. 2022

IJGM

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“…Previous studies have enunciated that miR-223-3p was involved in the progression of several cancers, including gastric cancer, 43 lung cancer, 44 and colon cancer. 45 The effects of miR-223-3p depend on the tumor cell type. Wang et al 46 have shown that miR-223-3p could hinder activity, migration, and invasion of BC cells.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, miR‐223‐3p expression was downregulated in BC tissues and cell lines. Previous studies have enunciated that miR‐223‐3p was involved in the progression of several cancers, including gastric cancer, 43 lung cancer, 44 and colon cancer 45 . The effects of miR‐223‐3p depend on the tumor cell type.…”

Section: Discussionmentioning

confidence: 99%

Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

et al. 2022

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Background Breast cancer (BC) is a common malignancy in women. Circular RNAs (circRNAs) have been reported to play a key role in the development of BC; however, the effect of circular RNA zinc finger RNA binding protein (circ_ZFR) in BC is unknown. Methods Abundances of circ_ZFR, fatty acid binding protein 7 (FABP7), and microRNA‐223‐3p (miR‐223‐3p) were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). The circular structure of circ_ZFR was validated by RNase R treatment. Cell proliferation, migration, invasion, and apoptosis were assessed by colony formation, cell counting kit‐8, Transwell, flow cytometry assays, respectively. All protein levels were determined by Western blot. Dual‐luciferase reporter assay was used to confirm the relationship between miR‐223‐3p and circ_ZFR or FABP7. A xenograft model was established to understand the effect of circ_ZFR on BC cell growth in vivo. Results The expression levels of circ_ZFR and FABP7 were higher in BC tissues and cell lines, whereas miR‐223‐3p expression was lower. Knockdown of circ_ZFR or FABP7 in BC cells reduced proliferation, migration, invasion, and epithelial mesenchymal transition (EMT), and induced apoptosis in vitro, whereas the opposite effects were observed in circ_ZFR‐overexpressed cells. Furthermore, circ_ZFR might act as a sponge for miR‐223‐3p to regulate FABP7 expression, thereby promoting the progression of BC cells in vitro and in vivo. Conclusion Circ_ZFR might act as a miRNA sponge for miR‐223‐3p to regulate FABP7, thereby promoting proliferation, migration, invasion, and EMT of BC cells, and inhibiting cell apoptosis.

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“…SOX11 promotes apoptosis of liver cancer cells through Wnt signaling pathway [ 25 ]. Overexpression of SOX11 can inhibit the invasion of colon cancer [ 26 ]. Through luciferase reporter gene experiments and Western blotting analysis, we found that SOX11 is the target gene of miR-937-3p.…”

Section: Discussionmentioning

confidence: 99%

MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma

Chen

et al. 2022

Cancer Cell Int

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Background Non-small cell lung cancer (NSCLC) is still one of the diseases with the highest mortality and morbidity, and lung adenocarcinoma (LUAD) accounts for more than half of all NSCLC cases in most countries. miRNA can be used as a potential biological marker and treatment for lung adenocarcinoma. However, the effect of miR-937-3p to the invasion and metastasis of LUAD cells is not clear. Methods miRNA microarray is used to analyze the expression of miRNA in lung adenocarcinoma tissue. Transwell migration, Wound-healing assay and Western blot analysis are used to analyze cell migration, invasion and epithelial-mesenchymal transition (EMT) capabilities. Tube formation is used to assess angiogenesis ability. In addition, dual luciferase reporter gene detection is used to identify the potential binding between miRNA and target mRNA. In vivo experiments were performed on male NOD/SCID nude mice by tail vein injection to establish a transplanted tumor model. The CHIP experiment is used to verify the transcription factors of miRNA. Result In our study, miR-937-3p was high-regulated in LUAD cell lines and tissues, and its expression level was related to tumor progression. We found that miR-937-3p high-expression has an effect on cell invasion and metastasis. In molecular mechanism, miR-937-3p causes SOX11 reduction by directly binding to the 3′-UTR of SOX11.In addition, MYC affects miR-937-3p transcription by binding to its promoter region. Conclusions Our research shows that miR-937-3p is mediated by MYC and can control the angiogenesis, invasion and metastasis of LUAD by regulating SOX11, thereby promoting the progress of LUAD. We speculate that miR-937-3p can be used as a therapeutic target and potential biomarker for LUAD.

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LINC00961 inhibits the migration and invasion of colon cancer cells by sponging miR‐223‐3p and targeting SOX11

Cited by 18 publications

References 30 publications

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

A Novel Defined Necroptosis-Related miRNAs Signature for Predicting the Prognosis of Colon Cancer

Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma

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