LINC00668 promotes tumorigenesis and progression through sponging miR-188–5p and regulating USP47 in colorectal cancer

Yan, Shuai; Yue, Yinzi; Wang, Jinbang; Li, Wenting; Sun, Mingming; Gu, Chao; Zeng, Li

doi:10.1016/j.ejphar.2019.172464

Cited by 44 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used the GEO database to select mRNAs that were downregulated in SOC, identified potential lncRNA-miRNA-mRNA interaction networks based on the presence of specific binding sites, and reconstructed a comprehensive ceRNA network. Several recent studies demonstrated that ceRNA-based mechanisms may operate in all types of carcinoma [5,6,[17][18][19][20][21][22][23]. In the present study, among the miRNAs found to be downregulated in SOC, hsa-miR-195-5p and hsa-miR-497-5p were predicted to bind to LINC00284.…”

Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

View full text Add to dashboard Cite

The low survival rate associated with serous ovarian carcinoma (SOC) is largely due to the lack of relevant molecular markers for early detection and therapy. Increasing experimental evidence has demonstrated that long noncoding RNAs (lncRNAs) are involved in cancer initiation and development, and a competitive endogenous RNA (ceRNA) hypothesis has been formulated. Therefore, the characterization of new lncRNA and lncRNA-related networks is crucial for early diagnosis and targeted therapy of SOC. Data on lncRNAs, mRNAs, and miRNAs with differential expression in SOC, compared to normal ovarian tissue, were obtained from the Gene Expression Omnibus (GEO) database. Data on lncRNA expression and clinical data in SOC were obtained from The Cancer Genome Atlas (TCGA). lncRNA-miRNA interactions were predicted by the miRBase database. Different online tools, i.e., TargetScan, RNA22, miRmap, microT, miRanda, StarBase, and PicTar, were cooperatively utilized to predict the mRNAs targeted by miRNAs. The plugin of BiNGO in Cytoscape and KOBAS 3.0 were used to conduct the functional and pathway enrichment analyses. The lncRNA, miRNAs, and mRNAs identified to be expressed at statistically significant and different levels between SOC and healthy fallopian tube tissues were further validated using qRT-PCR. A total of 4 lncRNAs (LINC00284, HAGLR, HCAT158, and BLACAT1) and 111 mRNAs were found to be upregulated in SOC tissues compared to normal tissues, based on the GEO database. LINC00284 was found to be highly expressed in SOC, in association with the upregulation of the transcription factor SOX9. The high LINC00284 expression was associated with poor prognosis and proved to be an independent risk factor in patients with SOC, based on TCGA database. The qRT-PCR validation results closely recapitulated the expression profiles and prognostic scores of the aforementioned bioinformatic analyses. The LINC00284-related ceRNA network was found to be associated with SOC carcinogenesis by biofunctional analysis. In conclusion, the LINC00284-related ceRNA network may provide valuable information on the mechanisms of SOC initiation and progression. Importantly, LINC00284 proved to be a new potential prognostic biomarker for SOC.

show abstract

Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It has been reported that LINC00578 is associated with worse OS in pancreatic cancer and lung adenocarcinoma [23,24]. LINC00668 promoted tumorigenesis and progression and indicated poor prognosis in not only breast cancer but also other cancers, such as colorectal cancer, hepatocellular carcinoma and non-small-cell lung cancer [25][26][27][28]. SEMA3B-AS1 might serve as a new tumor suppressor to inhibit the development of hepatocellular carcinoma, esophageal squamous cell carcinoma and gastric cardia adenocarcinoma [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of stemness-related lncRNA prognostic signature for breast cancer

et al. 2020

J Transl Med

View full text Add to dashboard Cite

Background: Long noncoding RNAs (lncRNAs) are emerging as crucial contributors to the development of breast cancer and are involved in the stemness regulation of breast cancer stem cells (BCSCs). LncRNAs are closely associated with the prognosis of breast cancer patients. It is critical to identify BCSC-related lncRNAs with prognostic value in breast cancer. Methods: A co-expression network of BCSC-related mRNAs-lncRNAs from The Cancer Genome Atlas (TCGA) was constructed. Univariate and multivariate Cox proportional hazards analyses were used to identify a stemness risk model with prognostic value. Kaplan-Meier analysis, univariate and multivariate Cox regression analyses and receiver operating characteristic (ROC) curve analysis were performed to validate the risk model. Principal component analysis (PCA) and Gene Set Enrichment Analysis (GSEA) functional annotation were conducted to analyze the risk model. Results: In this study, BCSC-related lncRNAs in breast cancer were identified. We evaluated the prognostic value of these BCSC-related lncRNAs and eventually obtained a prognostic risk model consisting of 12 BCSC-related lncR-NAs (Z68871.

show abstract

“…More and more cancer-related lncRNAs have been identified recently. Many lncRNAs were proved to have effects on HCC progression, among which, LINC00668 was found to serve as an oncogene in several cancers such as colorectal cancer [25] and lung adenocarcinoma [26]. LncRNA LINC00668 was reported to accelerate progression of breast cancer [27].…”

Section: Discussionmentioning

confidence: 99%

LncRNA LINC00668 promotes cell proliferation, migration, invasion ability and EMT process in hepatocellular carcinoma by targeting miR-532-5p/YY1 axis

2020

View full text Add to dashboard Cite

Liver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, the mechanism where LINC00668 regulates HCC is still unclear. qRT-PCR analysis was adopted to detect the expression of relative RNAs. Cytoplasmic and nuclear RNA fraction analysis was conducted to verify the underlying molecular mechanism. Cell colony formation was carried out to test cell colony formation ability and transwell assays were performed to testify cell migratory and invaded abilities. Relevant protein expression level was measured by Western blot assay. LINC00668 was significantly up-regulated in HCC tissues and cell lines. LINC00668 knockdown inhibited cell proliferative, migratory and invasion abilities and slowed down the epithelial–mesenchymal transition (EMT) process. Mechanistically, LINC00668 positively modulates the expression of YY1 by competitively binding to miR-532-5p. It was revealed that LINC00668 up-regulation accelerated cell proliferation and motility in HCC and suggested LINC00668 could be a potential therapeutic target for HCC.

show abstract

LINC00668 promotes tumorigenesis and progression through sponging miR-188–5p and regulating USP47 in colorectal cancer

Cited by 44 publications

References 36 publications

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Identification and validation of stemness-related lncRNA prognostic signature for breast cancer

LncRNA LINC00668 promotes cell proliferation, migration, invasion ability and EMT process in hepatocellular carcinoma by targeting miR-532-5p/YY1 axis

Contact Info

Product

Resources

About