LINC00624/TEX10/NF-κB axis promotes proliferation and migration of human prostate cancer cells

Zhou, Jian; Li, Juan; Qian, Chun-Ya; Qiu, Feng; Shen, Qianghua; Tong, Ruiqing; Yang, Qian; Xu, Jing; Zheng, Bo; Lv, Jinxing; Hou, Jianquan

doi:10.1016/j.bbrc.2022.02.078

Cited by 18 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present work, we identified three lncRNA markers of interest. The overexpression of LINC00624 has previously been shown to be of prognostic significance in liver cancer and, most recently, in PCa as well [43,44], thereby supporting the integrity of our experimental approach, as well as the importance of LINC00624 in prostate tumorigenesis. The fact that the expression of LINC00624 is significantly correlated in PCa tissues with that of the KRT8 gene provides further evidence that LINC00624 indeed contributes to PCa progression, possibly by the generation of more compact cell structures.…”

Section: Discussionsupporting

confidence: 71%

Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy

et al. 2023

View full text Add to dashboard Cite

Introduction: The clinical course of prostate cancer (PCa) is highly variable, ranging from indolent behavior to rapid metastatic progression. The Gleason score is widely accepted as the primary histologic assessment tool with significant prognostic value. However, additional biomarkers are required to better stratify patients, particularly those at intermediate risk. Methods: In this study, we analyzed the expression of 86 cancer hallmark genes in 171 patients with PCa who underwent radical prostatectomy and focused on the outcome of the 137 patients with postoperative R0-PSA0 status. Results: Low expression of the IGF1 and SRD52A, and high expression of TIMP2, PLAUR, S100A2, and CANX genes were associated with biochemical recurrence (BR), defined as an increase of prostate-specific antigen above 0.2 ng/mL. Furthermore, the analysis of the expression of 462 noncoding RNAs (ncRNA) in a sub-cohort of 39 patients with Gleason score 7 tumors revealed that high levels of expression of the ncRNAs LINC00624, LINC00593, LINC00482, and cd27-AS1 were significantly associated with BR. Our findings provide further evidence for tumor-promoting roles of ncRNAs in PCa patients at intermediate risk. The strong correlation between expression of LINC00624 and KRT8 gene, encoding a well-known cell surface protein present in PCa, further supports a potential contribution of this ncRNA to PCa progression. Conclusion: While larger and further studies are needed to define the role of these genes/ncRNA in PCa, our findings pave the way toward the identification of a subgroup of patients at intermediate risk who may benefit from adjuvant treatments and new therapeutic agents.

show abstract

Section: Discussionsupporting

confidence: 71%

Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy

et al. 2023

View full text Add to dashboard Cite

show abstract

“…SENP1 could contribute to LUAD patients' resistance to chemoradiotherapy, resulting in a poor prognosis [52]. Testis Expressed 10 (TEX10) exerted a negative role in human cancers through LINC00624/TEX10/NF-κB axis or lncRNA GATA3-AS1/miR-30b-5p/TEX10 axis, leading to NF-κB pathway activating and cancer cells migrating [53,54,55]. The reports regarding the effect of AMARCE1 and ZCWPW2 on the human tumor was limited.…”

Section: Discussionmentioning

confidence: 99%

Identification of a cuproptosis related chromatin regulators signature for lung adenocarcinoma

Wei

Yang

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Lung adenocarcinoma (LUAD) is the leading cause of cancer-related mortality worldwide, displaying dismal prognosis. Growing evidence now links cuproptosis related genes (CRGs) and chromatin regulators (CRs) to cancer progression. However, the relationship between CRGs and CRs is not yet fully understood. Therefore, we aimed to reveal the connection and prognostic value of these two factors in LUAD patients. Methods: Cuproptosis related genes (CRGs) and chromatin regulators (CRs) were retrieved from previous top studies. For TCGA analysis, mRNA expression andclinical information for LUAD were downloaded via R package. Functional enrichment was analyzed through the ClusterProfler package. Cox regression analysis and LASSO regression analysis were utilized to train models for predicting outcome in LUAD. The Kaplan-Meier curve analyses was used to assess the prognosis for cases categorized as High-risk and Low-risk groups. DsigDB dataset was performed to screen the potential drugs for LUAD treatment. Results: We visualized an protein-protein interaction (PPI) network between CRGs and CRs. Functional analysis suggested that CRs were mainly enriched in chromatin modification and cell cycle pathways. The prognosis prediction model was successfully constructed and validated for the patients with LUAD. Moreover, we also showed 11-CRs-based model was an independent prognostic factor for LUAD patients. This CR-based model was associated with immune cells infiltration as well as immune checkpoint. Seven prospective drugs was detected for the treatment of LUAD patients. Conclusion: In conclusion, our findings provide unique information not available in previous studies, casting novel insights about the correlation between CRGs and CRs in LUAD. We also identified several prognostic biomarkers for the survival of patients with LUAD.

show abstract

“…Sodium dodecyl sulfate (SDS) loading buffer was added to the magnetic beads and they were heated at 100°C for 10 min in a metal bath. The supernatant was then obtained for western blotting and liquid chromatography–tandem mass spectrometry (LC‐MC/MS), as previously reported 34,35 …”

Section: Methodsmentioning

confidence: 99%

E3 ubiquitin ligase RNF187 promotes growth of spermatogonia via lysine 48‐linked polyubiquitination‐mediated degradation of KRT36/KRT84

Yu,

Xu,

Gao

et al. 2023

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

Ubiquitination is the most common post‐translational modification and is essential for various cellular regulatory processes. RNF187, which is known as RING domain AP1 coactivator‐1, is a member of the RING finger family. RNF187 can promote the proliferation and migration of various tumor cells. However, whether it has a similar role in regulating spermatogonia is not clear. This study explored the role and molecular mechanism of RNF187 in a mouse spermatogonia cell line (GC‐1). We found that RNF187 knockdown reduced the proliferation and migration of GC‐1 cells and promoted their apoptosis. RNF187 overexpression significantly increased the proliferation and migration of GC‐1 cells. In addition, we identified Keratin36/Keratin84 (KRT36/KRT84) as interactors with RNF187 by co‐immunoprecipitation and mass spectrometry analyses. RNF187 promoted GC‐1 cell growth by degrading KRT36/KRT84 via lysine 48‐linked polyubiquitination. Subsequently, we found that KRT36 or KRT84 overexpression significantly attenuated proliferation and migration of RNF187‐overexpressing GC‐1 cells. In summary, our study explored the involvement of RNF187 in regulating the growth of spermatogonia via lysine 48‐linked polyubiquitination‐mediated degradation of KRT36/KRT84. This may provide a promising new strategy for treating infertility caused by abnormal spermatogonia development.

show abstract

LINC00624/TEX10/NF-κB axis promotes proliferation and migration of human prostate cancer cells

Cited by 18 publications

References 26 publications

Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy

Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy

Identification of a cuproptosis related chromatin regulators signature for lung adenocarcinoma

E3 ubiquitin ligase RNF187 promotes growth of spermatogonia via lysine 48‐linked polyubiquitination‐mediated degradation of KRT36/KRT84

Contact Info

Product

Resources

About