LINC00459 sponging miR-218 to elevate DKK3 inhibits proliferation and invasion in melanoma

Yang, Yuhua; Xu, Wenxian; Zheng, Zhuojun; Cao, Zhihai

doi:10.1038/s41598-019-55701-x

Cited by 13 publications

(14 citation statements)

References 39 publications

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“…Previous works have validated that lncRNAs have important functions in regulating malignant behaviors of melanoma. 19,21 For instance, lncRNA MEG3 is reported to promote melanoma proliferation and invasion by targeting miR_21/E-cadherin signaling. 19 LINC00459 is downregulated in melanoma and suppresses melanoma growth and metastasis via miR-218/DKK3 axis.…”

Section: Discussionmentioning

confidence: 99%

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<p>Long Noncoding RNA CAR10 Contributes to Melanoma Progression By Suppressing miR-125b-5p to Induce RAB3D Expression</p>

Xie¹,

Zheng²,

Xu³

et al. 2020

OTT

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Background: Melanoma is a very malignant skin cancer with high mortality and unsatisfactory prognosis. Many long noncoding RNAs (lncRNAs) have been reported to be aberrantly expressed in melanoma. How lncRNA regulates melanoma progression is poorly defined. LncRNA CAR10 has been shown to regulate the progression of several cancers and its role in melanoma remains unclear. This study aims to determine the role and mechanism of lncRNA CAR10 in the regulation of melanoma progression. Methods: qRT-PCR was utilized to analyze CAR10 in melanoma human tissues and cell lines while Kaplan-Meier curve was used to examine the survival rate. CCK8 assay and EdU assay were used to assess cell proliferation when Transwell assay was conducted to determine migration and invasion. And tumor xenograft assay was performed to evaluate tumor growth in vivo. Additionally, luciferase assay and RNA pulldown assay were performed to analyze the interactions among CAR10, miR-125b-5p and RAB3D. Results: LncRNA CAR10 was upregulated in melanoma tissues and cell lines. Upregulation of CAR10 predicted a poor prognosis in patients with melanoma. CAR10 knockdown suppressed proliferation, migration and invasion of melanoma cells in vitro. CAR10 silencing attenuated tumor growth in vivo. CAR10 inhibited miR-125b-5p activity to upregulate RAB3D expression. And miR-125b-5p/RAB3D signaling is crucial for CAR10-dependent melanoma progression. Conclusion: Our work suggests that lncRNA CAR10 promotes melanoma growth and metastasis through modulating miR-125b-5p/RAB3D axis.

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Section: Discussionmentioning

confidence: 99%

“…19 LINC00459 is downregulated in melanoma and suppresses melanoma growth and metastasis via miR-218/DKK3 axis. 21 LncRNA LINC00518 elevates the abilities of proliferation and metastasis through modulating miR-204-5p/AP1S2 pathway in melanoma. 22 Recently, CAR10 function is not fully researched.…”

Section: Discussionmentioning

confidence: 99%

<p>Long Noncoding RNA CAR10 Contributes to Melanoma Progression By Suppressing miR-125b-5p to Induce RAB3D Expression</p>

Xie¹,

Zheng²,

Xu³

et al. 2020

OTT

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“…Another major mechanism of lncRNAs is to directly interact with miRNAs and relieve the repressive roles of miRNAs on their targets. These lncRNAs are classed as ceRNAs [35][36][37]. lncRNA NEAT1 is reported to bind miR-495-3p and upregulate E2F3, a target of miR-495-3p [35].…”

Section: Discussionmentioning

confidence: 99%

Regulation of melanoma malignancy by the RP11-705C15.3/miR-145-5p/NRAS/MAPK signaling axis

Chen¹,

Li²,

Han³

et al. 2020

Cancer Gene Ther

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Melanoma is a common lethal skin cancer. Dissecting molecular mechanisms driving the malignancy of melanoma may uncover potential therapeutic targets. We previously identified miR-145-5p as an important tumor-suppressive microRNA in melanoma. Here, we further investigated the roles of long non-coding RNAs (lncRNAs) in melanoma. We identified RP11-705C15.3, a regulator of miR-145-5p, as an oncogenic lncRNA in melanoma. RP11-705C15.3 competitively bound miR-145-5p, relieved the repressive roles of miR-145-5p on its target NRAS, upregulated NRAS expression, and activated MAPK signaling. In vitro functional assays revealed that ectopic expression of RP11-705C15.3 promoted melanoma cell proliferation, inhibited apoptosis, and promoted migration and invasion. Silencing of RP11-705C15.3 repressed melanoma cell proliferation, induced apoptosis, and repressed migration and invasion. Notably, the roles of RP11-705C15.3 in melanoma cell proliferation, apoptosis, migration and invasion are reversed by miR-145-5p overexpression. In vivo functional assays revealed that RP11-705C15.3 promoted melanoma tumor growth and metastasis, which were also reversed by miR-145-5p overexpression. Furthermore, we investigated the expression of RP11-705C15.3 in clinical melanoma tissues and found that RP11-705C15.3 was increased in melanoma tissues. High expression of RP11-705C15.3 was positively correlated with thickness, ulceration, metastasis, and inferior overall survival. Taken together, our findings suggest RP11-705C15.3 as a novel oncogene in melanoma, and highlight that the RP11-705C15.3/miR-145-5p/NRAS/MAPK signaling axis may be potential therapeutic targets for melanoma.

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“…LINC00459 downregulated in melanoma sponges miR-218 which in turn downregulates dickkopf WNT signaling pathway inhibitor 3 (DKK3) [ 128 ]. DKK3 expression is usually lost at early stages of melanoma development [ 187 ].…”

Section: Competing Endogenous Rnas In Direct Interactions With Mirnasmentioning

confidence: 99%

“…DKK3 expression is usually lost at early stages of melanoma development [ 187 ]. LINC00459 low expression is correlated with decreased patient survival rates [ 128 ].…”

Section: Competing Endogenous Rnas In Direct Interactions With Mirnasmentioning

confidence: 99%

The Functional Role of Long Non-Coding RNAs in Melanoma

Wozniak

Czyż

2021

Cancers

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Melanoma is the most lethal skin cancer, with increasing incidence worldwide. The molecular events that drive melanoma development and progression have been extensively studied, resulting in significant improvements in diagnostics and therapeutic approaches. However, a high drug resistance to targeted therapies and adverse effects of immunotherapies are still a major challenge in melanoma treatment. Therefore, the elucidation of molecular mechanisms of melanomagenesis and cancer response to treatment is of great importance. Recently, many studies have revealed the close association of long noncoding RNAs (lncRNAs) with the development of many cancers, including melanoma. These RNA molecules are able to regulate a plethora of crucial cellular processes including proliferation, differentiation, migration, invasion and apoptosis through diverse mechanisms, and even slight dysregulation of their expression may lead to tumorigenesis. lncRNAs are able to bind to protein complexes, DNA and RNAs, affecting their stability, activity, and localization. They can also regulate gene expression in the nucleus. Several functions of lncRNAs are context-dependent. This review summarizes current knowledge regarding the involvement of lncRNAs in melanoma. Their possible role as prognostic markers of melanoma response to treatment and in resistance to therapy is also discussed

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LINC00459 sponging miR-218 to elevate DKK3 inhibits proliferation and invasion in melanoma

Cited by 13 publications

References 39 publications

<p>Long Noncoding RNA CAR10 Contributes to Melanoma Progression By Suppressing miR-125b-5p to Induce RAB3D Expression</p>

<p>Long Noncoding RNA CAR10 Contributes to Melanoma Progression By Suppressing miR-125b-5p to Induce RAB3D Expression</p>

Regulation of melanoma malignancy by the RP11-705C15.3/miR-145-5p/NRAS/MAPK signaling axis

The Functional Role of Long Non-Coding RNAs in Melanoma

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