LINC00324 accelerates the proliferation and migration of osteosarcoma through regulating WDR66

Abstract

Osteosarcoma (OS) is a type of malignancy featured with high morbidity and easy metastasis. Although past years have witnessed the great improvement in the treatments of OS, there remains a long way to go. Therefore, further research on the underlying molecular mechanism of OS progression is in imminent need. Long noncoding RNAs (lncRNA) are recognized as a cluster of transcripts over 200 bases.Increasing studies have unveiled their significant regulatory roles in cancers, including in osteosarcoma. Long intergenic non-protein coding RNA 324 (LINC00324) is a newly identified lncRNA exerting oncogenic functions in several cancers, but its role in OS is yet to be uncovered. Therefore, the present study planned to explore the role of LINC00324 in osteosarcoma. We first validated the upregulation of LINC00324 in OS tissues and cell lines and established its correlation with OS tumor progression and metastasis. Importantly, the prognostic significance of LINC00324 was identified in patients with OS. Gain-and loss-of-function assays revealed that LINC00324 accelerated cell proliferation and migration in OS. Mechanistically, we revealed that LINC00324 stabilized WD repeat-containing protein 66 (WDR66) messenger RNA through interacting with Hu antigen R. Rescue assays verified that WDR66 was required for the regulation of LINC00324 in promoting proliferation and migration of OS cells. In conclusion, the present study proved that LINC00324 accelerated the proliferation and migration of osteosarcoma cells through regulating WDR66, providing a new prognostic target for osteosarcoma. K E Y W O R D SLINC00324, migration, osteosarcoma, proliferation, WDR66

Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1

“…Previous researches have confirmed that LINC00324 takes part in cell proliferation, invasion, and migration in cancer. Wu et al have proven that LINC00324 increases the proliferation and migration of osteosarcoma cells via targeting WDR66 [9]. Pan et al have shown that LINC00324 has the ability to promote the growth of lung adenocarcinoma through regulating the miR-615-5p/AKT1 axis [8].…”

“…Recently, some studies have indicated that LINC00324 is involved in cancer progression. For example, LINC00324 increases the proliferation and migration of osteosarcoma cells [9]. LINC00324 promotes the tumorigenesis of lung adenocarcinoma [8].…”

Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c

“…Recent studies indicate that aberrant expression of lncRNAs facilitates OS development (Ren et al, 2019;Wu et al, 2020;Yang et al, 2019;Zhang et al, 2019). These lncRNAs exert their functions by several mechanisms, including targeting some host genes, involvement with signaling pathways and competing with endogenous miRNA.…”

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

“…In addition, expression of LINC00324 has been validated as an independent predictive factor of overall survival and disease-free survival among patients with gastric cancer [26]. LINC00324 is also overexpressed in lung adenocarcinoma [27] and osteosarcoma [28]. Overexpression of LINC00324 is closely associated with tumor size, distant metastasis, TNM stage, differentiation degree, and poor prognosis among patients with osteosarcoma [28].…”

“…Long intergenic non-protein-coding RNA 324 (LINC00324) is abnormally expressed in multiple human cancer types [26][27][28] and performs important functions in cancer initiation and progression. Nevertheless, the expression profile, clinical relevance, specific effects, and regulatory mechanisms underlying the action of LINC00324 in RB have not been fully elucidated.…”