LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway

Abstract

Hepatocellular carcinoma (HCC) is well known as the sixth most common malignant tumor and the third leading cause of cancer-related deaths globally. LINC00152 was documented as an important long non-coding RNA (lncRNA) involved in the pathogenesis of gastric cancer; however, the detailed mechanism of action of LINC00152 remains unknown. Here, based on the increased level of LINC00152 in HCC tissues, we found that LINC00152 could promote cell proliferation in vitro and tumor growth in vivo. Furthermore, microarray-based analysis indicated that LINC00152 could activate the mechanistic target of rapamycin(mTOR) pathway by binding to the promoter of EpCAM through a cis-regulation, as confirmed by Gal4-λN/BoxB reporter system. Thus, LINC00152 might be involved in the oncogenesis of HCC by activating the mTOR signaling pathway and might be a novel index for clinical diagnosis in the future.

The significance of low levels of LINC RP1130-1 expression in human hepatocellular carcinoma

“…The lncRNA BANCR has been found to offer potential as a diagnostic marker of lung cancer (26), Zfas1 offers potential as a marker of breast cancer (27), and TUG1 offers potential as a diagnostic marker of bladder cancer (28). In addition, the lncRNAs PVT1 (29), HULC (30), HEIH (31), ATB (32), DANCR (33), and LINC00152 (34) have been found to be dys-regulated in conditions like HCC.…”

Long Noncoding RNA Linc00152 Functions as a Tumor Propellant in Pan-Cancer

“…5C). A previous study showed that the promoter region of linc00152 was hypomethylated in hepatic carcinoma tissues than in para-cancer tissues via bisulfite sequencing polymerase chain reaction [22] (Fig. 5D).…”

“…(C) Linc00152 expression was inversely correlated with DNA methylation of the linc00152 locus in breast cancer in TCGA. Linc00152 was hypo-methylated in hepatic carcinoma tissues compared with para-cancer tissues via bisulfite sequencing polymerase chain reaction [22]. (E) DNA methylation in the linc00152 locus in pan-cancer tissues and that in normal tissues in 450K data from TCGA.…”

Short-lived non-coding transcripts (SLiTs): Clues to regulatory long non-coding RNA

“…which causes activation of Phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling (26). LINC00152 is involved in the oncogenesis of hepatocellular carcinoma by activating the mechanistic target of the rapamycin (mTOR) signaling pathway (27).…”