LINC00052 upregulates EPB41L3 to inhibit migration and invasion of hepatocellular carcinoma by binding miR-452-5p

Abstract

Numerous studies have demonstrated that a class of long noncoding RNAs (lncRNAs) are dysregulated in hepatocellular carcinoma (HCC) and they are closely related with tumorigenesis. Our previous studies indicated that LINC00052 was a downregulated lncRNA in HCC and acted as a tumor suppressor gene. Using transcription microarray analysis, we found that knockdown of LINC00052 resulted in EPB41L3 downregulation. However, the function of EPB41L3 and the mechanism of LINC00052 downregulating EPB41L3 in HCC remain unclear. In this study, we found that overexpression of LINC00052 could upregulate the EPB41L3 expression and it might serve as a tumor suppressor gene in HCC. Database analysis showed that miR-452-5P could target LINC00052. The binding regions between LINC00052 and miR-452-5P were confirmed by luciferase assays. Moreover, LINC00052 inhibited cell malignant behavior by increasing miR-452-5P expression, suggesting that LINC00052 was negatively regulated by miR-452-5P. In addition, overexpression of miR-452-5P resulted in a decrease of EPB41L3 expression, suggesting that EPB41L3 was as a target of miR-452-5P. In conclusion, these results demonstrated that a novel pathway was mediated by LINC00052 in HCC.

A SOX9‐AS1/miR‐5590‐3p/SOX9 positive feedback loop drives tumor growth and metastasis in hepatocellular carcinoma through the Wnt/β‐catenin pathway

“…In our study, the non‐effect of SOX9‐AS1 on the SOX9 promoter and the cytoplasmic expression of SOX9‐AS1 indicated that SOX9‐AS1 might regulate SOX9 at the post‐transcriptional level. An increasing number of studies have revealed that lncRNA could regulate gene expression through sponging miRNA in HCC (Lu et al ., ; Zhu et al ., ). We identified 47 miRNA interacting with both SOX9‐AS1 and SOX9 through bioinformatics tools and picked the five most downregulated miRNA in order to investigate their interaction with SOX9‐AS1.…”

“…For example, in HCC, lncRNA NNT‐AS1 promoted progression and metastasis by regulating the miR‐363/CDK6 axis (Lu et al ., ). LINC00052 regulated EPB41L3 by targeting miR‐452‐5p to inhibit HCC (Zhu et al ., ). The miRNA are conserved non‐coding transcripts with about 22 nucleotides, responsible for silencing gene expression through targeting 3′UTR of mRNA (Bartel, ; Brümmer and Hausser, ).…”

Retracted: Long noncoding RNA LINC00052 inhibits colorectal cancer metastasis by sponging microRNA‐574‐5p to modulate CALCOCO1 expression

“…8 Moreover, it could bind to miR-452-5p as a sponge and downregulated its target gene, erythrocyte membrane protein band 4.1 like 3 (EPB41L3), in HCC. 9 In addition to its effects in HCC, researchers found that LINC00052 had a significant effect on gastric cancer and breast cancer. [10][11][12] Despite these findings, LINC00052's function in CRC has not been studied.…”

Peer Review #2 of "Comprehensive analysis of lncRNA-associated competing endogenous RNA network in tongue squamous cell carcinoma (v0.2)"

“…The function of LINC00052 varies depending on the location of tumors. It acts as a tumor suppressor through inhibiting cell proliferation, invasion and migration in hepatocellular carcinoma (Xiong et al 2016;Yan et al 2018;Zhu et al 2017), whereas it could promote breast cancer growth (Salameh et al 2017) and gastric cell metastasis and proliferation (Shan et al 2017).…”