lin-4 and the NRDE pathway are required to activate a transgenic lin-4 reporter but not the endogenous lin-4 locus in C. elegans

Jiao, Alan; Foster, Daniel J.; Dixon, Julia; Slack, Frank J.

doi:10.1371/journal.pone.0190766

Cited by 5 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and muscle paramyosin was used to track conversion to muscle fate (Horner et al, 1998;Yuzyuk et al, 2009). We concentrated on endogenous fate markers because exogenous reporters can sometimes lead to spurious expression or regulation (Jiao et al, 2018;Mango, 2007).…”

Section: Met-2 Promotes Loss Of Plasticitymentioning

confidence: 99%

Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis

et al. 2019

View full text Add to dashboard Cite

During the first hours of embryogenesis, formation of higher-order heterochromatin coincides with the loss of developmental potential. Here, we examine the relationship between these two events, and we probe the processes that contribute to the timing of their onset. Mutations that disrupt histone H3 lysine 9 (H3K9) methyltransferases reveal that the methyltransferase MET-2 helps terminate developmental plasticity, through mono-and di-methylation of H3K9 (me1/me2), and promotes heterochromatin formation, through H3K9me3. Although loss of H3K9me3 perturbs formation of higher-order heterochromatin, embryos are still able to terminate plasticity, indicating that the two processes can be uncoupled. Methylated H3K9 appears gradually in developing C. elegans embryos and depends on nuclear localization of MET-2. We find that the timing of H3K9me2 and nuclear MET-2 is sensitive to rapid cell cycles, but not to zygotic genome activation or cell counting. These data reveal distinct roles for different H3K9 methylation states in the generation of heterochromatin and loss of developmental plasticity by MET-2, and identify the cell cycle as a crucial parameter of MET-2 regulation.

show abstract

Section: Met-2 Promotes Loss Of Plasticitymentioning

confidence: 99%

Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Another abundant miRNA is lin-4-5p: originally identified in C. elegans in relation to developmental timing 26 , it shows complete seed identity with miR-125 of several parasitic helminths and humans. The dendrogram obtained from the comparison of the available orthologues showed similarities between ape-lin-4 in A. suum and B. malayi (Fig.…”

Section: Resultsmentioning

confidence: 99%

A miRNAs catalogue from third-stage larvae and extracellular vesicles of Anisakis pegreffii provides new clues for host-parasite interplay

Cavallero

Bellini

Pizzarelli

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Anisakids are widespread marine parasites of medical, veterinary and economic relevance. They infect marine natural hosts but humans can accidentally acquire the fish-borne zoonosis anisakiasis by ingesting infected raw fishes or mollusks. Among the several species described, Anisakis pegreffii is one of the main etiological agent of the disease, in particular in the Mediterranean area. Despite the growing evidence of miRNAs involvement in host-parasite interplay, and the emerging role of exosomal microvesicles in shuttling them between different cell types (and sometime across species), no information on miRNAs from any Anisakis species is presently available. In this study we isolated extracellular vesicles (EVs) released by Anisakis pegreffii infective third-stage larvae (L3) and analyzed by RNA-seq small RNAs from both L3 and EVs. We showed by nanoparticle tracking analysis that L3 release in culture medium particles of size compatible with the one of extracellular vesicles. A catalogue of 156 miRNAs from A. pegreffii was compiled by sequence comparison to evolutionary close species and miRNA prediction software. Using differential expression analysis, we identified a small number of highly abundant miRNAs in larvae and extracellular vesicles fractions whose potential biological relevance may deserve future investigation. Finally, A. pegreffii miRNAs were compared to those described in other parasitic helminths and predicted targets among human genes were searched, suggesting their potential involvement during infection.

show abstract

“…The foregut marker PHA-4 was used to identify cells that retained their endogenous identity and resisted exogenous HLH-1, and muscle paramyosin was used to track conversion to muscle fate (Horner et al, 1998;Yuzyuk et al, 2009). We concentrated on endogenous fate markers because exogenous reporters can sometimes lead to spurious expression or regulation (Jiao et al, 2018;Mango, 2007). As a control, we examined hlh-1 mRNA expression before and after heat shock and observed no difference in induction between wild-type and mutant embryos ( Figure 1D).…”

Section: Met-2 Promotes Loss Of Plasticitymentioning

confidence: 99%

MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Mutlu

Chen

Hall³

2018

Preprint

View full text Add to dashboard Cite

STATEMENTDuring early embryogenesis, heterochromatin formation and loss of developmental plasticity are coordinately regulated by distinct Histone H3 Lysine 9 (H3K9) methylation states, by the methyltransferase MET-2. ABSTRACTDuring the first hours of embryogenesis, formation of higher-order heterochromatin coincides with the loss of developmental potential. Here we examine the relationship between these two processes, and we probe the determinants that contribute to their onset. Mutations that disrupt histone H3 lysine 9 (H3K9) methyltransferases reveal that the methyltransferase MET-2 helps terminate developmental plasticity, likely through mono-and di-methylation of H3K9 (me1/me2), and promotes heterochromatin formation, likely through H3K9me3. We examine how MET-2 is regulated and find that methylated H3K9 appears gradually and depends on the accumulated time of embryogenesis.H3K9me is independent of zygotic genome activation or cell counting. These data reveal how central events are synchronized during embryogenesis and distinguish distinct roles for different H3K9 methylation states.

show abstract

lin-4 and the NRDE pathway are required to activate a transgenic lin-4 reporter but not the endogenous lin-4 locus in C. elegans

Cited by 5 publications

References 21 publications

Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis

Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis

A miRNAs catalogue from third-stage larvae and extracellular vesicles of Anisakis pegreffii provides new clues for host-parasite interplay

MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Contact Info

Product

Resources

About