lin-17/Frizzled and lin-18 regulate POP-1/TCF-1 localization and cell type specification during C. elegans vulval development

Deshpande, Rashmi; Inoué, Takao; Priess, James R; Hill, Russell J.

doi:10.1016/j.ydbio.2004.10.020

Cited by 29 publications

(30 citation statements)

References 43 publications

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“…1 F and G) (26), cog-1 and lin-11, in turn, control the expression pattern of egl-17 and cdh-3. Patterns of egl-17 and cdh-3 expression observed in lin-17 and lin-18 mutants are consistent with high levels of cog-1 and lin-11 turning on the expression of these genes (31,32). Another set of cell-fatepatterning mechanisms controlling gene expression was revealed by the analysis of vulE vs. vulF fate specification using the zmp-1 reporter.…”

Section: Resultsmentioning

confidence: 52%

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Transcriptional network underlying Caenorhabditis elegans vulval development

Inoué

Wang

Ririe

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The vulval development of Caenorhabditis elegans provides an opportunity to investigate genetic networks that control gene expression during organogenesis. During the fourth larval stage (L4), seven vulval cell types are produced, each of which executes a distinct gene expression program. We analyze how the expression of cell-type-specific genes is regulated. Ras and Wnt signaling pathways play major roles in generating the spatial pattern of cell types and regulate gene expression through a network of transcription factors. One transcription factor (lin-29) primarily controls the temporal expression pattern. Other transcription factors (lin-11, cog-1, and egl-38) act in combination to control cell-typespecific gene expression. The complexity of the network arises in part because of the dynamic nature of gene expression, in part because of the presence of seven cell types, and also because there are multiple regulatory paths for gene expression within each cell type.organogenesis ͉ signaling pathways ͉ transcription D evelopmental events are driven by spatially and temporally regulated gene expression. Understanding how complex patterns of expression are produced is therefore a critical part of deciphering mechanisms of development. In general, intercellular signaling mechanisms interact with a network of transcription factors to generate cell-type-specific patterns of gene expression. The late stage of Caenorhabditis elegans vulval development offers a useful model in which to study this process. During this period of vulval development, seven distinct cell types are produced that express unique combinations of genes. Over the last several years, a number of genes were discovered that are expressed in cell-type and stage-specific patterns in the vulva, and several transcription factors were found to regulate these genes. In this paper, we synthesize and extend our current knowledge of this genetic network.The C. elegans vulva connects the uterine lumen to the outside, allowing for passage of sperm and fertilized eggs (1). Vulval cells are generated postembryonically from precursor cells P3.p P4.p, P5.p, P6.p, P7.p, and P8.p [also called vulval precursor cells (VPC)]. During the mid-third larval (L3) stage, EGF and Notch signaling induces the middle three VPCs (P5.p, P6.p, and P7.p) to adopt vulval fates, whereas P3.p, P4.p, and P8.p fuse with the hypodermal syncytium, hyp7 (2-6).During the late-L3 to the early-L4 stage, P5.p, P6.p, and P7.p undergo two or three rounds of cell division to produce 22 nuclei (7) (Fig. 1A). These nuclei are in cells of seven types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), as evidenced by subsequent morphogenetic movements and by the pattern of gene expression (8, 9) (Fig. 1B). The seven cell types that are present in the adult vulva represent specializations within the general epithelial cell class. These cells exhibit cell-type general features; for example, each expresses ajm-1, a component of the apical junction that connects neighboring cells in epithelial tissues (8)....

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Section: Resultsmentioning

confidence: 52%

“…1 A). Although the full mechanism that establishes this pattern is not known, Wnt signals, mediated by lin-17 (Frizzled-type Wnt receptor) and lin-18 (Ryk-type Wnt receptor), control the anterior͞posterior order of cell types among P7.p descendants (31,32) (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Transcriptional network underlying Caenorhabditis elegans vulval development

Inoué

Wang

Ririe

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…More recent work using gene expression markers to look at cell-fate patterning in the P7.p lineage confirms the polarity reversal in lin-17 mutants (4,5). For example, although only P7.pa descendants express egl-17::gfp in the wild type (9), in some lin-17 mutants only P7.pp descendants express egl-17::gfp, indicating reversal of fate between P7.pa and P7.pp.…”

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confidence: 89%

“…In Caenorhabditis elegans, Wnt signaling controls the anterior/ posterior (a/p) polarity of the vulval precursor cell P7.p and the patterning of its descendants (3)(4)(5)(6). During wild-type vulval development, the P7.p cell becomes polarized (i.e., asymmetric) along the a/p axis and after three rounds of division gives rise to asymmetrically patterned tissue in which different cell types are arranged in a specific anterior-to-posterior sequence (7,8).…”

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confidence: 99%

p21-Activated kinase interacts with Wnt signaling to regulate tissue polarity and gene expression

Goh

Hagen

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Wnt signaling is mediated by three classes of receptors, Frizzled, Ryk, and Ror. In Caenorhabditis elegans, Wnt signaling regulates the anterior/posterior polarity of the P7.p vulval lineage, and mutations in lin-17/Frizzled cause loss or reversal of P7.p lineage polarity. We found that pak-1/Pak (p21-activated kinase), along with putative activators of Pak, nck-1/Nck, and ced-10/Rac, regulates P7.p polarity. Mutations in these genes suppress the polarity defect of lin-17 mutants. Furthermore, mutations in pak-1, nck-1, and ced-10 cause constitutive dauer formation at 27°C, a phenotype also observed in egl-20/Wnt and cam-1/Ror mutants. In HEK293T cells, Pak1 can antagonize canonical Wnt signaling. Moreover, overexpression of Ror2 leads to phosphorylation of Pak1. Together, these results indicate that Pak interacts with Wnt signaling to regulate tissue polarity and gene expression.cell polarity | β-catenin | T-cell factor | patterning | asymmetry

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“…Both the lin-17 and lin-18 genes encode Wnt receptors that regulate asymmetric divisions in vulval development. [34][35][36][37] Further analysis of the roles of TOE-2 in the Q and vulval lineages should determine whether TOE-2 is a novel player in the Wnt signaling pathway.…”

Section: Toe-2 and Mpk-1 Signalingmentioning

confidence: 99%