Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments

Poughon, Laurent; Laroche, Céline; Creuly, Catherine; Dussap, Claude-Gilles; Paille, Christel; Lasseur, Christophe; Monsieurs, Pieter; Heylen, Wietse; Coninx, Ilse; Mastroleo, Felice; Leys, Natalie

doi:10.1016/j.lssr.2020.03.002

Cited by 36 publications

(38 citation statements)

References 18 publications

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“…The photosynthetic cyanobacterium Limnospira indica , formerly known as Arthrospira sp. PCC8005 ( Nowicka-Krawczyk et al, 2019 ) is used as part of the O 2 and edible biomass production in one of the PBRs ( Poughon et al, 2020 ).…”

Section: Bioregenerative Air Revitalization In Space Habitatsmentioning

confidence: 99%

“…Therefore, the productivities primarily depend on culture design (illuminated surface versus culture volume) and operational variable (light energy flux). A departure value of light energy yield by photosynthetic systems is in the order of magnitude of 20 mol of photons per mol of carbon fixed ( Cornet and Dussap, 2009 ; Poughon et al, 2020 ). This is consistent with the maximum value of 69 g m –2 d –1 reported by Morais et al (2009) with a maximum possible light intensity before the appearance of photo inhibition.…”

Section: Photosynthetic Microorganisms As Catalysers For Air Revitalization In Spacementioning

confidence: 99%

“…In a next step, a suitable kinetic model for all important parameters (e.g., in the case of cyanobacteria: O 2 production, CO 2 uptake, biomass production, use of nutrients, etc.) has to be developed and tested ( Poughon et al, 2020 ). Only if the organism suits all requirements, experiments with fed-batch or continuous liquid cultures can start.…”

Section: Development Of Biological Air Revitalization Systems For Spacementioning

confidence: 99%

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Use of Photobioreactors in Regenerative Life Support Systems for Human Space Exploration

et al. 2021

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There are still many challenges to overcome for human space exploration beyond low Earth orbit (LEO) (e.g., to the Moon) and for long-term missions (e.g., to Mars). One of the biggest problems is the reliable air, water and food supply for the crew. Bioregenerative life support systems (BLSS) aim to overcome these challenges using bioreactors for waste treatment, air and water revitalization as well as food production. In this review we focus on the microbial photosynthetic bioprocess and photobioreactors in space, which allow removal of toxic carbon dioxide (CO2) and production of oxygen (O2) and edible biomass. This paper gives an overview of the conducted space experiments in LEO with photobioreactors and the precursor work (on ground and in space) for BLSS projects over the last 30 years. We discuss the different hardware approaches as well as the organisms tested for these bioreactors. Even though a lot of experiments showed successful biological air revitalization on ground, the transfer to the space environment is far from trivial. For example, gas-liquid transfer phenomena are different under microgravity conditions which inevitably can affect the cultivation process and the oxygen production. In this review, we also highlight the missing expertise in this research field to pave the way for future space photobioreactor development and we point to future experiments needed to master the challenge of a fully functional BLSS.

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Section: Bioregenerative Air Revitalization In Space Habitatsmentioning

confidence: 99%

Section: Photosynthetic Microorganisms As Catalysers For Air Revitalization In Spacementioning

confidence: 99%

Section: Development Of Biological Air Revitalization Systems For Spacementioning

confidence: 99%

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Use of Photobioreactors in Regenerative Life Support Systems for Human Space Exploration

et al. 2021

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“…Other experiments, including not only algae but also other organisms, have been flown over the last decades, for example the Closed Equilibrated Biological Aquatic System (CEBAS) (Blüm 2003), Omegahab (Anken 2008), and Closed Aquatic Ecosystem (CAES) (Wang et al, 2008). Only two experiments have focused to date on the PBR technology in microgravity conditions, artemiss with 30 days's cultivation of Spirulina (Poughon et al, 2020) and PBR@LSR with two week's cultivation of Chlorella (Detrell et al, 2020b).…”

Section: Microalgae Researchmentioning

confidence: 99%

“…PC technologies are not able to produce food, which can only be achieved using biological organisms. Several candidates are currently being investigated, with a main focus on higher plants (Kittang et al, 2014;Hamilton et al, 2020) and microalgae (Detrell et al, 2020b;Poughon et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chlorella Vulgaris Photobioreactor for Oxygen and Food Production on a Moon Base—Potential and Challenges

Detrell

2021

Front. Astron. Space Sci.

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A base on the Moon surface or a mission to Mars are potential destinations for human spaceflight, according to current space agencies’ plans. These scenarios pose several new challenges, since the environmental and operational conditions of the mission will strongly differ than those on the International Space Station (ISS). One critical parameter will be the increased mission duration and further distance from Earth, requiring a Life Support System (LSS) as independent as possible from Earth’s resources. Current LSS physico-chemical technologies at the ISS can recycle 90% of water and regain 42% of O2 from the astronaut’s exhaled CO2, but they are not able to produce food, which can currently only be achieved using biology. A future LSS will most likely include some of these technologies currently in use, but will also need to include biological components. A potential biological candidate are microalgae, which compared to higher plants, offer a higher harvest index, higher biomass productivity and require less water. Several algal species have already been investigated for space applications in the last decades, being Chlorella vulgaris a promising and widely researched species. C. vulgaris is a spherical single cell organism, with a mean diameter of 6 µm. It can grow in a wide range of pH and temperature levels and CO2 concentrations and it shows a high resistance to cross contamination and to mechanical shear stress, making it an ideal organism for long-term LSS. In order to continuously and efficiently produce the oxygen and food required for the LSS, the microalgae need to grow in a well-controlled and stable environment. Therefore, besides the biological aspects, the design of the cultivation system, the Photobioreactor (PBR), is also crucial. Even if research both on C. vulgaris and in general about PBRs has been carried out for decades, several challenges both in the biological and technological aspects need to be solved, before a PBR can be used as part of the LSS in a Moon base. Those include: radiation effects on algae, operation under partial gravity, selection of the required hardware for cultivation and food processing, system automation and long-term performance and stability.

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Fluorescence and electron transfer of Limnospira indica functionalized biophotoelectrodes

Ryzhkov,

Colson,

Ahmed

et al. 2024

Photosynth Res

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Cyanobacteria play a crucial role in global carbon and nitrogen cycles through photosynthesis, making them valuable subjects for understanding the factors influencing their light utilization efficiency. Photosynthetic microorganisms offer a promising avenue for sustainable energy conversion in the field of photovoltaics. It was demonstrated before that application of an external electric field to the microbial biofilm or cell improves electron transfer kinetics and, consequently, efficiency of power generation. We have integrated live cyanobacterial cultures into photovoltaic devices by embedding Limnospira indica PCC 8005 cyanobacteria in agar and PEDOT:PSS matrices on the surface of boron-doped diamond electrodes. We have subjected them to varying external polarizations while simultaneously measuring current response and photosynthetic performance. For the latter, we employed Pulse-Amplitude-Modulation (PAM) fluorometry as a non-invasive and real-time monitoring tool. Our study demonstrates an improved light utilization efficiency for L. indica PCC 8005 when immobilized in a conductive matrix, particularly so for low-intensity light. Simultaneously, the impact of electrical polarization as an environmental factor influencing the photosynthetic apparatus diminishes as matrix conductivity increases. This results in only a slight decrease in light utilization efficiency for the illuminated sample compared to the dark-adapted state.

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Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments

Cited by 36 publications

References 18 publications

Use of Photobioreactors in Regenerative Life Support Systems for Human Space Exploration

Use of Photobioreactors in Regenerative Life Support Systems for Human Space Exploration

Chlorella Vulgaris Photobioreactor for Oxygen and Food Production on a Moon Base—Potential and Challenges

Fluorescence and electron transfer of Limnospira indica functionalized biophotoelectrodes

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