Limnohabitans curvus gen. nov., sp. nov., a planktonic bacterium isolated from a freshwater lake

Hahn, Martin W.; Kasalický, Vojtěch; Jezbera, Jan; Brandt, Ulrike; Jezberová, Jitka; Šimek, Karel

doi:10.1099/ijs.0.013292-0

Cited by 93 publications

(78 citation statements)

References 37 publications

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“…Sequences matching fresh water chemo-organotrophic bacteria including Limnohabitans, ACK-M1 and C39 were abundant in the 0.2 µm filter size class (representing the small cell sized and free-living bacteria) in March, which is consistent with previous observations that these organisms are capable of efficient assimilation of organic carbon and inorganic nutrients facilitating rapid growth in response to terrestrial water intrusions (Hahn et al 2010, Lin et al 2012.…”

Section: Changes In Bacterial Community Composition During Inflow Eventssupporting

confidence: 79%

River bacterioplankton community responses to a high inflow event

Carney¹,

Mitrovic²,

Jeffries³

et al. 2015

Aquat. Microb. Ecol.

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Microbes drive chemical cycling and productivity within river ecosystems, but their influence may shift when intense allochthonous inputs accompany high freshwater inflow (flood) events. Investigating how floods influence microbial processes is fundamentally important for our understanding of river ecology, but is generally overlooked. We analysed bacterioplankton community composition (BCC) and abundance over 4 mo following an enormous flood event in the Hunter River, Australia, that resulted in a major fish kill. Concentrations of dissolved organic carbon (DOC) and inorganic nutrients (N and P) were up to 3 times higher during the flood event compared to prior and subsequent months. Bacterial cell abundances were up to 10 times higher at impacted sites during the flood event. Using Automated Ribosomal Intergenic Spacer Analysis we found significant shifts in BCC between the flood impacted month and subsequent months (p < 0.05). Distance linear modelling indicated that DOC and dissolved N and P correlated most strongly with BCC patterns during the high inflow, whereas community dynamics correlated most strongly with nitrogen oxides and ammonium during the river's recovery phase. 16S rRNA amplicon pyrosequencing revealed that common soil-associated and facultative anaerobic genera of Proteobacteria were most dominant during the flood period, suggesting that a proportion of the bacterial community observed during this event were potentially inactive soil microbes transported into the river via terrestrial runoff. During the recovery period, Cyanobacteria and freshwater-associated genera of Actinobacteria and Proteobacteria became dominant in 16S rRNA pyrosequencing profiles. These observations indicate that allochthonous nutrients delivered via floods can significantly stimulate bacterial growth, underpinning substrate-controlled succession of bacterial communities and ultimately shaping the ecology within river ecosystems.

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Section: Changes In Bacterial Community Composition During Inflow Eventssupporting

confidence: 79%

River bacterioplankton community responses to a high inflow event

Carney¹,

Mitrovic²,

Jeffries³

et al. 2015

Aquat. Microb. Ecol.

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“…The first described species, L. curvus, is a chemoorganotrophic, aerobic, and facultative anaerobe capable of assimilating glucose and a variety of small organic acids such as acetate, pyruvate, and fumarate but not amino acids (76). Notably, L. parvus cannot assimilate acetate, while the other three species are capable of doing so (113).…”

Section: Class Betaproteobacteriamentioning

confidence: 99%

“…The four described Limnohabitans species within the betI lineage can be discriminated based on their substrate utilization characteristics as well as their 16S rRNA gene sequences and extent of genomic DNA-DNA hybridization (76,77,113). The first described species, L. curvus, is a chemoorganotrophic, aerobic, and facultative anaerobe capable of assimilating glucose and a variety of small organic acids such as acetate, pyruvate, and fumarate but not amino acids (76).…”

Section: Class Betaproteobacteriamentioning

confidence: 99%

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A Guide to the Natural History of Freshwater Lake Bacteria

Newton

Jones

Eiler

et al. 2011

Microbiol Mol Biol Rev

1,279

122

1,414

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SUMMARY Freshwater bacteria are at the hub of biogeochemical cycles and control water quality in lakes. Despite this, little is known about the identity and ecology of functionally significant lake bacteria. Molecular studies have identified many abundant lake bacteria, but there is a large variation in the taxonomic or phylogenetic breadths among the methods used for this exploration. Because of this, an inconsistent and overlapping naming structure has developed for freshwater bacteria, creating a significant obstacle to identifying coherent ecological traits among these groups. A discourse that unites the field is sorely needed. Here we present a new freshwater lake phylogeny constructed from all published 16S rRNA gene sequences from lake epilimnia and propose a unifying vocabulary to discuss freshwater taxa. With this new vocabulary in place, we review the current information on the ecology, ecophysiology, and distribution of lake bacteria and highlight newly identified phylotypes. In the second part of our review, we conduct meta-analyses on the compiled data, identifying distribution patterns for bacterial phylotypes among biomes and across environmental gradients in lakes. We conclude by emphasizing the role that this review can play in providing a coherent framework for future studies.

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“…In freshwater, one of the key bacterioplankton groups within the Betaproteobacteria is represented by the genus Limnohabitans (8,18), the major part of which belongs to the cluster R-BT065 (36), further identified as the RBT lineage. The RBT lineage is currently represented by the species Limnohabitans planktonicus and Limnohabitans parvus (18).…”

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confidence: 99%

Alga-Derived Substrates Select for Distinct Betaproteobacterial Lineages and Contribute to Niche Separation in Limnohabitans Strains

Šimek

Kasalický

Zapomělová

et al. 2011

Appl Environ Microbiol

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We examined the proportions of major Betaproteobacteria subgroups within bacterial communities in diverse nonaxenic, monospecific cultures of algae or cyanobacteria: four species of cryptophyta (genera Cryptomonas and Rhodomonas), four species of chlorophyta (genera Pediastrum, Staurastrum, and Chlamydomonas), and two species of cyanobacteria (genera Dolichospermum and Aphanizomenon). In the cryptophyta cultures, Betaproteobacteria represented 48 to 71% of total bacteria, the genus Limnohabitans represented 18 to 26%, and the Polynucleobacter B subcluster represented 5 to 16%. In the taxonomically diverse chlorophyta group, the genus Limnohabitans accounted for 7 to 45% of total bacteria. In contrast, cyanobacterial cultures contained significantly lower proportions of the Limnohabitans bacteria (1 to 3% of the total) than the cryptophyta and chlorophyta cultures. Notably, largely absent in all of the cultures was Polynucleobacter necessarius (Polynucleobacter C subcluster). Subsequently, we examined the growth of Limnohabitans strains in the presence of different algae or their extracellular products (EPP). Two strains, affiliated with Limnohabitans planktonicus and Limnohabitans parvus, were separately inoculated into axenic cultures of three algal species growing in an inorganic medium: Cryptomonas sp., Chlamydomonas noctigama, and Pediastrum boryanum. The Limnohabitans strains cocultured with these algae or inoculated into their EPP consistently showed (i) pronounced population growth compared to the control without the algae or EPP and (ii) stronger growth stimulation of L. planktonicus than of L. parvus. Overall, growth responses of the Limnohabitans strains cultured with algae were highly species specific, which suggests a pronounced niche separation between two closely related Limnohabitans species likely mediated by different abilities to utilize the substrates produced by different algal species.There is compelling evidence that phytoplankton community dynamics have a significant impact on the composition of bacterioplankton communities (for example, see references 6, 19, and 21). The apparent driving force of such alga-bacterium interactions is likely the nature and quantity of alga-derived substrates available in the form of extracellular phytoplankton products (EPP) or decaying algal biomass. Although it is usually not known which algal species are the major EPP producers in situ, tight species-specific alga-bacterium relationships have been suggested as characterizing bacterium-alga consortia (e.g., 6, 21, 33). Interactions of phytoplankton and bacteria range from symbiotic to parasitic relationships (3). It is not surprising, then, that specific bacterial assemblages associated with different algae can also stimulate or even inhibit algal growth, as documented for cultures of marine diatoms (6). The latter study also demonstrated that free-living and phytoplankton-associated (i.e., attached to algal surfaces) bacteria are significantly different from each other and are dominated by distinct phylog...

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Limnohabitans curvus gen. nov., sp. nov., a planktonic bacterium isolated from a freshwater lake

Cited by 93 publications

References 37 publications

River bacterioplankton community responses to a high inflow event

River bacterioplankton community responses to a high inflow event

A Guide to the Natural History of Freshwater Lake Bacteria

Alga-Derived Substrates Select for Distinct Betaproteobacterial Lineages and Contribute to Niche Separation in Limnohabitans Strains

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