Limits of Survival of the Mingled Rumen Bacteria in the Washed Cell Suspension of Rumen Ciliate Protozoa

Onodera, Ryoji; Yamaguchi, Haruko; Eguchi, Chiaki; Kandatsu, Makoto

doi:10.1080/00021369.1977.10862882

Cited by 16 publications

(16 citation statements)

References 2 publications

(2 reference statements)

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“…To determine the protozoal numbers, 0.5 ml portions of the microbial suspensions were collected, mixed with 4.5 ml of methylgreen-formalin-salt (MFS) solution (Onodera et al, 1977), and kept at room temperature. Rumen protozoa were counted with the aid of a Fuchs-Rosenthal hemocytometer.…”

Section: Methodsmentioning

confidence: 99%

Tryptophan biosynthesis and production of other related compounds from indolepyruvic acid by mixed ruminal bacteria, protozoa, and their mixture in vitro.

Mohammed

Onodera

Khan

1999

J. Gen. Appl. Microbiol.

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Section: Methodsmentioning

confidence: 99%

Tryptophan biosynthesis and production of other related compounds from indolepyruvic acid by mixed ruminal bacteria, protozoa, and their mixture in vitro.

Mohammed

Onodera

Khan

1999

J. Gen. Appl. Microbiol.

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“…In order to determine the protozoal numbers, 0.5 ml portions of the microbial suspensions were collected, mixed with 4.5 ml of methylgreen-formalin-salt (MFS) solution (Onodera et al, 1977) and kept at room temperature.…”

Section: Methodsmentioning

confidence: 99%

In vitro metabolism of phenylalanine by ruminal bacteria, protozoa, and their mixture.

Amin

Onodera

1997

J. Gen. Appl. Microbiol.

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An in vitro study was conducted to examine the metabolism of phenylalanine (Phe) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed lucerne cubes (Medicago sativa) and a concentrated mixture twice a day. Microbial suspensions were anaerobically incubated at 39°C for 12 h. Phe and some other related compounds in both supernatants and microbial hydrolysates of the incubations were analysed by HPLC. The net degradation rate (µmollg microbial nitrogen) of Phe in B was about 1.5-fold higher than that in P. Phe was converted mainly into phenylacetic acid (PAA) and unknown compound(s) that presumably involved tyrosine in B, P, and BP during the 12h incubation period. Small amounts of benzoic acid (BZA), and traces of phenylpropionic acid (PPR) and phenyllactic acid (PLA) were also produced from Phe. PAA production in B was found to be higher than that in P, whereas it was significantly higher in BP. Although BZA production was less than one-tenth that of PAA production, it was higher in P than in B and BP. PPR was detected in both B and BP, but not in P. PLA was detected only in B. The production of unknown compound(s) was higher in B than in P and BP.

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“…Ruminal NH 3 -N concentration was measured colorimetrically with the use of spectrophotometer (Alpha-1101 model; Labnics Equipment, Fremont, CA, USA) using commercial lab test (SPINREACT, St. Esteve de Bas, Girona, Spain) as described by Konitzer and Voigt (1963). Aliquots of 2 ml subsample were mixed with 4 ml of methyl green-formalin-saline solution in glass bottle at room temperature for subsequent analyses of total protozoa counts (Onodera et al, 1977) using Neubauer improved bright-line counting chamber according to the procedure described by Dehority (1993). Individual SCFA concentrations were determined as described by Abo-Zeid et al (2017) with some modifications using gas chromatography (GC) (TRACE1300, Thermo Fisher Scientific, Inc., Rodano, Milan, Italy) fitted with an AS3800 autosampler and equipped with a capillary column HP- FFAP was used as carrier gas.…”

Section: Rumen Fermentation Characteristics Analysesmentioning

confidence: 99%

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

El-Zaiat¹,

Morsy²,

Elwakeel³

et al. 2018

J. Anim. Feed Sci.

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Limits of Survival of the Mingled Rumen Bacteria in the Washed Cell Suspension of Rumen Ciliate Protozoa

Cited by 16 publications

References 2 publications

Tryptophan biosynthesis and production of other related compounds from indolepyruvic acid by mixed ruminal bacteria, protozoa, and their mixture in vitro.

Tryptophan biosynthesis and production of other related compounds from indolepyruvic acid by mixed ruminal bacteria, protozoa, and their mixture in vitro.

In vitro metabolism of phenylalanine by ruminal bacteria, protozoa, and their mixture.

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

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