Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

Schmid, Daniela; Brown, Denise R.; Nisenbaum, Rosane; Burke, Rae Lyn; Alexander, D. L.; Ashley, Rhoda; Pellett, Philip E.; Reeves, William C.

doi:10.1128/jcm.37.2.376-379.1999

Cited by 59 publications

(5 citation statements)

References 29 publications

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“…mgG-2 has hitherto been shown to be the only HSV-2 protein which induces a type-specific antibody response. However, one problem when using mgG-2 as a type-specific antigen is the recently reported limitations in sensitivity (Schmid et al ., 1999 ). In addition, the antibody response to this protein develops relatively late after onset of primary HSV-2 infection (up to 6 months) as compared to the antibody response to other envelope proteins, for example glycoprotein B (Ashley et al ., 1988 ; Lopez et al ., 1993 ).…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants

Liljeqvist¹,

Trybala²,

Hoebeke

et al. 2002

Journal of General Virology

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Glycoprotein G-2 (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted aminoterminal portion (sgG-2) and to a cell-associated carboxy-terminal portion which is further Oglycosylated to constitute the mature gG-2 (mgG-2). In contrast to mgG-2, which is known to elicit a type-specific antibody response in the human host, information on the immunogenic properties of sgG-2 is lacking. Here the sgG-2 protein was purified on a heparin column and used for production of monoclonal antibodies (mAbs). Four anti-sgG-2 mAbs were mapped using a Pepscan technique and identified linear epitopes which localized to the carboxy-terminal part of the protein. One additional anti-sgG-2 mAb, recognizing a non-linear epitope, was reactive to three discrete peptide stretches where the most carboxy-terminally located stretch was constituted by the amino acids 320 RRAL 323. Although sgG-2 is rapidly secreted into the cell-culture medium after infection, the anti-sgG-2 mAbs identified substantial amounts of sgG-2 in the cytoplasm of HSV-2-infected cells. All of the anti-sgG-2 mAbs were HSV-2 specific showing no cross-reactivity to HSV-1 antigen or to HSV-1-infected cells. Similarly, sera from 50 HSV-2 isolation positive patients were all reactive to sgG-2 in an enzyme immunoassay whilst no reactivity was seen in 25 sera from HSV-1 isolation positive patients or in 25 serum samples from HSV-negative patients suggesting that sgG-2 is a novel antigen potentially suitable for type-discriminating serodiagnosis.

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Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants

Liljeqvist¹,

Trybala²,

Hoebeke

et al. 2002

Journal of General Virology

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“…After all personal identifiers were removed, the specimens were examined for antibodies to HIV-1 and herpes simplex virus 2 (HSV-2) at CDC in Atlanta. HSV-2 serologic testing was performed by a method described elsewhere (Schmid et al, 1999;CDC, 1993 No. 542).…”

Section: Serologic Assessmentmentioning

confidence: 99%

Planning STI/HIV Prevention Among Refugees and Mobile Populations: Situation Assessment of Sudanese Refugees

Holt

Effler²,

Brady

et al. 2003

Disasters

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This article reflects an investigation of knowledge, attitudes and behaviours and HIV/STI prevalence of Sudanese refugees and Ethiopian sex workers in 1992. It represents one of the earliest such investigations within an African refugee population. The investigation took place in the Dimma refugee settlement in south-western Ethiopia and study participants included Sudanese refugee men and women and Ethiopian female sex workers. Methods used for this investigation included focus group discussions, behavioural surveys and serologic testing. The main outcome measures of the investigation were HIV/STI knowledge, attitudes and behaviours and biological markers for HIV, syphilis and herpes simplex 2. The study findings indicate that in the early 1990s, knowledge about AIDS and condom use was low among Sudanese refugee women and not one reported having ever used a condom. Furthermore, sexual contact between refugee men and sex workers was frequent during the time of this study and the prevalence of HIV and other STIs was high. The results confirm a widely held assumption that highly mobile and transient populations in Africa are susceptible to STIs and HIV, in large part due to their knowledge, attitudes and behaviours.

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“…Recently, type-specific anti-HSV assays have become available commercially, being able to differentiate between antibodies against HSV-1 and HSV-2. HSV-2 antibodies are usually detectable within 8 weeks of acquisition, and antibodies are generally considered to persist throughout life, although loss of HSV-2 antibodies over time has been reported [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of, and risk factors for, HSV-2 antibodies in sexually transmitted disease patients, healthy pregnant females, blood donors and medical students in Tanzania and Norway

et al. 2005

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The prevalence of specific HSV-2 antibodies was studied in Tanzanian and Norwegian sexually transmitted disease (STD) patients (1095) and non-STD patients (488). Correlates to demographic and behavioural factors were evaluated. Seropositivity was determined by the non-commercial peptide-55 enzyme-linked immunoassay. The prevalence of HSV-2 antibodies was 70% in Tanzanian and 17% in Norwegian STD patients, 35% in Tanzanian blood donors and pregnant women, and 4, 7 and 14% in Norwegian medical students, blood donors and pregnant women respectively. A higher HSV-2 prevalence was associated with female sex, increasing age, previous STDs, history of genital HSV infection, coitarchal age (age at first intercourse) <15 years and HIV seropositivity. Compared to previous data, the prevalence of HSV-2 antibodies in Tanzanian STD patients has increased remarkably. In Norwegian STD patients our results are consistent with, or lower than, the prevalence previously reported in Western Europe. Demographic rather than behavioural factors were associated with higher prevalence of HSV-2 antibodies in STD patients.

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Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

Cited by 59 publications

References 29 publications

Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants

Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants

Planning STI/HIV Prevention Among Refugees and Mobile Populations: Situation Assessment of Sudanese Refugees

Prevalence of, and risk factors for, HSV-2 antibodies in sexually transmitted disease patients, healthy pregnant females, blood donors and medical students in Tanzania and Norway

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