Limiting acetoin generation during 2,3-butanediol fermentation with Paenibacillus polymyxa using lignocellulosic hydrolysates

Stoklosa, Ryan J.; García-Negrón, Valerie; Latona, Renee J.; Toht, Matthew

doi:10.1016/j.biortech.2023.130053

Cited by 2 publications

(1 citation statement)

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“…This decrease in OD during aerobic fermentation has been observed previously as a function of glucose depletion. , As shown later in Figure , glucose depletion does not necessarily indicate that fermentation ends. Without glucose, P. polymyxa can redirect metabolic functions to consume 2,3-BDO for conversion to acetoin . The fermentation conducted under anoxic conditions in the anaerobic bottles show fast biomass growth within 24 h for the hydrolysate media procured from 0.15 g of NaOH per g of dry biomass pretreated CMH, while the 0.3 g of NaOH per g of dry biomass pretreatment condition hydrolysate produced a slower rate of biomass growth.…”

Section: Resultsmentioning

confidence: 97%

Hemp Cryo-milling and the Impact of Alkaline Pretreatment on Biochemical Conversion

Stoklosa,

Latona,

Berger

et al. 2024

ACS Sustainable Resour. Manage.

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Cannabis sativa (hemp) is a fiber crop that has historically been used for manufacturing textiles. Recently, limitations on cultivating industrial hemp in the United States have been lifted, as hemp has been reclassified as an agricultural commodity with the potential to serve as a bioenergy crop. This work used liquid nitrogen (LN 2 ) to cryo-mill hemp (CMH) before NaOH chemical pretreatment. Pretreatments were conducted at 10% (w/v) solids loading with NaOH loadings ranging from 0 to 0.4 g of NaOH/g of dry biomass. Increasing NaOH chemical loading removed up to 80% percent of the original hemicellulose, but delignification leveled off around 20% even at the highest NaOH loadings. Moderate NaOH pretreatment conditions (0.1 to 0.2 g of NaOH/g of dry biomass) provided optimal conditions for sugar recovery following enzymatic hydrolysis with glucan yields approaching 90% and xylan yields at 80%. Pretreated CMH hydrolysate was fermented by Paenibacillus polymyxa to produce 2,3-butanediol (2,3-BDO). Under anoxic conditions, P. polymyxa generated 19 g/L of 2,3-BDO without conversion into acetoin. Aerobic fermentations produced 2,3-BDO titers near 13 g/L after 24 h, however, the resulting 2,3-BDO was converted to acetoin reaching around 9 g/L after 144 h.

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Section: Resultsmentioning

confidence: 97%