Limited-view light sheet fluorescence microscopy for three dimensional volume imaging

Rasmi, C. K.; Mohan, Kavya; Madhangi, M; Rajan, K.; Nongthomba, Upendra; Mondal, Partha Pratim

doi:10.1063/1.4938536

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…The last decade has seen an explosion of light sheet variants that can be adapted for applications requiring optical manipulation. Some of these include thin light-sheet microscopy 39 , ultramicroscopy 40 , objective coupled planar illumination microscopy (OCPI) 41 , confocal light-sheet microscopy 42 , 43 , multiple light-sheet microscopy 44 , dual-inverted selective-plane illumination microscopy (diSPIM) 45 , light-sheet theta microscopy (LSTM) 46 , open-top light-sheet (OTLS) 47 , 48 and lattice light sheet microscopy 49 , LVLSM 50 and IML-SPIM 29 . These variants offer many useful configuration for generating light sheets that may be suitable for realizing application-specific optical manipulation system.…”

Section: Introductionmentioning

confidence: 99%

Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells

Mondal

Baro

Singh

et al. 2022

Sci Rep

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Optical trapping and patterning cells or microscopic particles is fascinating. We developed a light sheet-based optical tweezer to trap dielectric particles and live HeLa cells. The technique requires the generation of a tightly focussed diffraction-limited light-sheet realized by a combination of cylindrical lens and high NA objective lens. The resultant field is a focussed line (along x-axis) perpendicular to the beam propagation direction (z-axis). This is unlike traditional optical tweezers that are fundamentally point-traps and can trap one particle at a time. Several spherical beads undergoing Brownian motion in the solution are trapped by the lightsheet gradient potential, and the time (to reach trap-centre) is estimated from the video captured at 230 frames/s. High-speed imaging of beads with increasing laser power shows a steady increase in trap stiffness with a maximum of 0.00118 pN/nm at 52.5 mW. This is order less than the traditional point-traps, and hence may be suitable for applications requiring delicate optical forces. On the brighter side, light sheet tweezer (LOT) can simultaneously trap multiple objects with the distinct ability to manipulate them in the transverse (xy) plane via translation and rotation. However, the trapped beads displayed free movement along the light-sheet axis (x-axis), exhibiting a single degree of freedom. Furthermore, the tweezer is used to trap and pattern live HeLa cells in various shapes and structures. Subsequently, the cells were cultured for a prolonged period of time (> 18 h), and cell viability was ascertained. We anticipate that LOT can be used to study constrained dynamics of microscopic particles and help understand the patterned cell growth that has implications in optical imaging, microscopy, and cell biology.

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Section: Introductionmentioning

confidence: 99%

Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells

Mondal

Baro

Singh

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Some of these include thin light-sheet microscopy 12 , ultramicroscopy 13 , objective coupled planar illumination microscopy (OCPI) 14 , confocal light-sheet microscopy 15 , 16 , multiple light-sheet microscopy 17 , dual-inverted selective-plane illumination microscopy (diSPIM) 18 , light-sheet theta microscopy (LSTM) 19 , open-top light-sheet (OTLS) 20 , 21 , coded light-sheet array microscopy (CLAM) 22 and lattice light sheet microscopy 23 . The advantages offered by LSFM and its variants have seen an upsurge in the studies related to large biological specimens 9 , 24 – 26 . Recently few groups (Keller, Fraser and Huiskin) have shown impressive time-series of entire embryo 27 – 31 .…”

Section: Introductionmentioning

confidence: 99%

Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms

Basumatary

Ara

Mukherjee

et al. 2021

Sci Rep

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Continuous monitoring of large specimens for long durations requires fast volume imaging. This is essential for understanding the processes occurring during the developmental stages of multicellular organisms. One of the key obstacles of fluorescence based prolonged monitoring and data collection is photobleaching. To capture the biological processes and simultaneously overcome the effect of bleaching, we developed single- and multi-color lightsheet based OVSS imaging technique that enables rapid screening of multiple tissues in an organism. Our approach based on OVSS imaging employs quantized step rotation of the specimen to record 2D angular data that reduces data acquisition time when compared to the existing light sheet imaging system (SPIM). A co-planar multicolor light sheet PSF is introduced to illuminate the tissues labelled with spectrally-separated fluorescent probes. The detection is carried out using a dual-channel sub-system that can simultaneously record spectrally separate volume stacks of the target organ. Arduino-based control systems were employed to automatize and control the volume data acquisition process. To illustrate the advantages of our approach, we have noninvasively imaged the Drosophila larvae and Zebrafish embryo. Dynamic studies of multiple organs (muscle and yolk-sac) in Zebrafish for a prolonged duration (5 days) were carried out to understand muscle structuring (Dystrophin, microfibers), primitive Macrophages (in yolk-sac) and inter-dependent lipid and protein-based metabolism. The volume-based study, intensity line-plots and inter-dependence ratio analysis allowed us to understand the transition from lipid-based metabolism to protein-based metabolism during early development (Pharyngula period with a critical transition time, $$\tau _c = 50$$ τ c = 50 h post-fertilization) in Zebrafish. The advantage of multicolor lightsheet illumination, fast volume scanning, simultaneous visualization of multiple organs and an order-less photobleaching makes OVSS imaging the system of choice for rapid monitoring and real-time assessment of macroscopic biological organisms with microscopic resolution.

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“…SPIM [10][11][12] uses a sheet of light to illuminate samples, as proposed by Voie et al [13] and further improved by the Stelzer group [14,15]. While SPIM is generally regarded as a fast scanning technique compared to point scanning techniques [16,17], recent studies demonstrate even faster setups by using for example multiple sheets [18], and many extensions have been developed such as Limited-View LSM [19] and Tiling Lightsheet SPIM [20]. Fast and efficient illumination restricted to the observed focal volume additionally minimizes photobleaching and phototoxicity [21,22].…”

Section: Introductionmentioning

confidence: 99%

Fast quantitative time lapse displacement imaging of endothelial cell invasion

et al. 2020

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In order to unravel rapid mechano-chemical feedback mechanisms in sprouting angiogenesis, we combine selective plane illumination microscopy (SPIM) and tailored image registration algorithms -further referred to as SPIM-based displacement microscopy -with an in vitro model of angiogenesis. SPIM successfully tackles the problem of imaging large volumes while upholding the spatial resolution required for the analysis of matrix displacements at a subcellular level. Applied to in vitro angiogenic sprouts, this unique methodological combination relates subcellular activity -minute to second time scale growing and retracting of protrusions -of a multicellular systems to the surrounding matrix deformations with an exceptional temporal resolution of 1 minute for a stack with multiple sprouts simultaneously or every 4 seconds for a single sprout, which is 20 times faster than with a conventional confocal setup. Our study reveals collective but non-synchronised, non-continuous activity of adjacent sprouting cells along with correlations between matrix deformations and protrusion dynamics. OPEN ACCESS Citation: Steuwe C, Vaeyens M-M, Jorge-Peñas A, Cokelaere C, Hofkens J, Roeffaers MBJ, et al. (2020) Fast quantitative time lapse displacement imaging of endothelial cell invasion. PLoS ONE 15 (1): e0227286. https://doi.org/10.

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Limited-view light sheet fluorescence microscopy for three dimensional volume imaging

Cited by 9 publications

References 29 publications

Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells

Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells

Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms

Fast quantitative time lapse displacement imaging of endothelial cell invasion

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