Limited ecological risk of insect-resistance transgene flow from cultivated rice to its wild ancestor based on life-cycle fitness assessment

Li, Lei; Yang, Xiao; Wang, Lei; Yan, Huanxin; Su, Jun; Wang, Zhen; Lu, Bao‐Rong

doi:10.1007/s11434-016-1152-5

Cited by 8 publications

(16 citation statements)

References 49 publications

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“…The most pertinent use of this tool is to estimate transgene flow frequencies from GE crops to their non-GE counterparts and to wild/weedy relative species, which is important to assess social-economic and particularly environmental impacts caused by transgene flow as discussed previously ( Ellstrand 1992 , 2003 ; Yong and Kim 2001 ; Lu and Snow 2005 ; Loureiro et al 2009 ; Lu and Yang 2009 ). If a transgene can move from a GE crop to its wild/weedy relatives at a comparatively high frequency ( Song et al 2003 a ; Chen et al 2004 ; Weekes et al 2005 ) and bring considerable fitness effects (benefit or cost) to the wild or weedy relatives ( Yang et al 2011 ; Li et al 2016 ), rigorous biosafety measures should be taken to minimize the undesired environmental consequences ( Lu and Snow 2005 ; Lu and Yang 2009 ; Lu et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Model-based calculating tool for pollen-mediated gene flow frequencies in plants

Wang

2016

AoB PLANTS

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This study provide a model-based calculating tool/software that can accurately calculate pollen-mediated gene flow (PMGF) frequencies of wind-pollinated plant species by including five biological and climatic parameters. The calculating tool can easily be used by any users who are not familiar with mathematical modeling. The required parameters can be measured directly in the field without conducting PMGF experiments, which makes this tool practical to estimate PMGF frequencies. This tool will be helpful to estimate transgene flow and its potential impacts, in addition to determining isolation distances between GE and non-GE crops.

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Section: Discussionmentioning

confidence: 99%

Model-based calculating tool for pollen-mediated gene flow frequencies in plants

Wang

2016

AoB PLANTS

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“…Studies have shown that the major crop species, including wheat, rice, maize, soybean, and oil rapes, can hybridize spontaneously with their wild relatives occurring in the vicinity [3,[9][10][11][12][13]. Pollen-mediated crop-to-wild transgene flow or hybridization may result in unwanted environmental and ecological consequences if the transgene acquired by the wild relative populations can confer considerable fitness benefits or costs [4,6,8,[14][15][16]. Therefore, assessing the potential environmental impact caused by transgene flow becomes a basic requirement as a regulatory procedure before any GE crop is cultivated for commercial production.…”

Section: Introductionmentioning

confidence: 99%

“…Usually, transgenic fitness is estimated involving hybrid descendants derived from artificial crosses between a GE crop and wild relative species, simulating transgene flow from a GE crop to wild populations [14,15,19,21,24]. Undoubtedly, results generated from these studies have contributed significantly to the assessment of the potential environmental impact caused by transgene flow.…”

Section: Introductionmentioning

confidence: 99%

“…Undoubtedly, results generated from these studies have contributed significantly to the assessment of the potential environmental impact caused by transgene flow. Nearly all the studies focused only on the comparison of survival and fecundity-related characteristics between GE ad non-GE crop-wild hybrid lineages, particularly on the number of seeds produced by plants containing a transgene [14,15,19,21]. Such comparison is based on the general assumption that the number of seeds produced by a plant is the key to determining the population size of the crop-wild hybrids.…”

Section: Introductionmentioning

confidence: 99%

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Increased Longevity and Dormancy of Soil-Buried Seeds from Advanced Crop–Wild Rice Hybrids Overexpressing the EPSPS Transgene

Jiang

Yang

2021

Biology

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Estimating the fitness effect conferred by a transgene introgressed into populations of wild relative species from a genetically engineered (GE) crop plays an important role in assessing the potential environmental risks caused by transgene flow. Such estimation has essentially focused on the survival and fecundity-related characteristics measured above the ground, but with little attention to the fate of GE seeds shattered in the soil seed banks after maturation. To explore the survival and longevity of GE seeds in soil, we examined the germination behaviors of crop–wild hybrid seeds (F4–F6) from the lineages of a GE herbicide-tolerant rice (Oryzasativa) line that contains an endogenous EPSPS transgene hybridized with two wild O. rufipogon populations after the seeds were buried in soil. The results showed significantly increased germination of the GE crop–wild hybrid seeds after soil burial, compared with that of the non-GE hybrid seeds. Additionally, the proportion of dormant seeds and the content of the growth hormone auxin (indole-3-acetic acid, IAA) in the GE crop–wild hybrid seeds significantly increased. Evidently, the EPSPS transgene enhances the survival and longevity of GE crop–wild rice seeds in the soil seed banks. The enhanced survival and longevity of the GE hybrid seeds is likely associated with the increases in seed dormancy and auxin (IAA) by overexpressing the rice endogenous EPSPS transgene. Thus, the fate of GE seeds in the soil seed banks should be earnestly considered when assessing the environmental risks caused by transgene flow.

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“…In addition, the Bt/CpTI transgene was still effective in later generations derived from crosses between transgenic rice and weedy rice or wild rice, resulting in lower insect damage and increased fecundity under high insect pressure. 8,12,13 However, previous studies have indicated that the transgenic rice and hybrid progeny with the exogenous Bt gene usually had an obvious yield disadvantage compared with the parental rice line in the field or greenhouse with relatively low insect pressure conditions. [14][15][16][17][18] Liang et al 19 found that the empty grain number per spike of transgenic cry1Ab/c rice HH1 was significantly higher than that of parent rice MH63 under the field conditions with low insect pressure sprayed with insecticide, and the grain filling rate of HH1 was lower than that of MH63.…”

Section: Introductionmentioning

confidence: 99%

Enhanced yield performance of transgenic cry1C* rice in saline-alkaline soil

Liu

2020

GM Crops & Food

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China has a large area of saline-alkaline land that can be utilized for the cultivation of transgenic rice. Therefore, the growth and reproductive behavior of transgenic rice are not only a problem for production that needs to be resolved, but also an important aspect of environmental risk assessment for saline alkali soil. In the present study, an insect-resistant transgenic cry1C* rice, T1C-19, was grown in farmland and saline-alkaline soils. The transcription and translation of the exogenous cry1C*, and vegetative and reproductive fitness, such as plant height, tiller number, biomass, filled grain number and weight per plant, were assessed. Our findings indicated that the transcription and translation of exogenous cry1C* gene in T1C-19 rice grown in saline-alkaline soil were lower than that grown in farmland; however, the correlation was not significant. The vegetative and reproductive growth abilities of T1C-19 were lower than that of the parental rice, Minghui63 (MH63), in farmland. In alkaline-saline soil, except for tiller number and biomass, there were no significant differences between T1C-19 and MH63 in other vegetative indices. In contrast, the reproductive indices of T1C-19 were significantly higher than those of MH63. The results suggested that T1C-19 had a strong reproductive capacity, and significantly reduced the loss of yield caused by insects, thereby leading to a higher yield than that of MH63 grown in saline-alkaline soils. This may promote the cultivation of saline-alkaline soil to permit farming of T1C-19 in China in the future, despite the possible increased ecological risks.

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Limited ecological risk of insect-resistance transgene flow from cultivated rice to its wild ancestor based on life-cycle fitness assessment

Cited by 8 publications

References 49 publications

Model-based calculating tool for pollen-mediated gene flow frequencies in plants

Model-based calculating tool for pollen-mediated gene flow frequencies in plants

Increased Longevity and Dormancy of Soil-Buried Seeds from Advanced Crop–Wild Rice Hybrids Overexpressing the EPSPS Transgene

Enhanced yield performance of transgenic cry1C* rice in saline-alkaline soil

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