Limited diversity of the immunoglobulin heavy chain variable domain of the emerald rockcod Trematomus bernacchii

Coscia, Maria Rosaria; Oreste, Umberto

doi:10.1006/fsim.2002.0418

Cited by 18 publications

(8 citation statements)

References 52 publications

(66 reference statements)

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“…In T. bernacchii, IgM molecules have been extensively studied (Pucci et al 2003;Abelli et al 2005 among others) and both VH and CH genes have been investigated (Coscia et al 2000;Oreste and Coscia 2002;Coscia and Oreste 2003).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish

et al. 2006

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The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish

et al. 2006

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“…The proportions of each JH segment were very different, however. JH1 (24.4%), JH2 (22.2%), and JH6 (26.6%) were the three major segments found to be equally associated to different VH segments of both families [25].…”

Section: Diversity Of the Heavy Chain Variable Regionmentioning

confidence: 98%

“…In some fish species such as salmonids [35], ictalurids [36], and cyprinids [37], the molecular structure of the IgH gene locus has suggested the possibility that duplication events could have shaped their present organization. To [25]; D. rerio CDR2 sequences are from [29]; O. mykiss CDR2 sequences are from [30]; G. morhua CDR2 sequences are from [31]. C. hamatus CDR2 sequences have been deposited under the GenBank accession numbers HQ384351eHQ384364.…”

Section: Diversity Of the Heavy Chain Constant Regionmentioning

confidence: 99%

“…To define the basis for the generation of antibody diversity in T. bernacchii, a spleen library was constructed by 5 0 RACE and fortyfive cDNA clones, containing complete or partial sequences of rearranged VH/D/JH segments, were analyzed [25]. All the VH sequences analyzed shared, on average, 80% of nucleotide identity.…”

Section: Diversity Of the Heavy Chain Variable Regionmentioning

confidence: 99%

“…Based on the percentage of nucleotide identity, VH sequences were grouped into two families: most sequences (89%), fell into a family referred to as Trbe VH II; the remaining sequences belonged to the Trbe VH I family. In particular, the Trbe VH II family could be divided into two subfamilies: Trbe VH IIa, comprising 30 different VH gene segments sharing 81e99% nucleotide identity, and Trbe VH IIb consisting of only 5 gene segments with a nucleotide identity between 82 and 99% [25]. A Neighbor-Joining (NJ) tree showing the relative distances between the VH families was constructed on the basis of multiple alignment of the T. bernacchii cDNA VH sequences generated by the ClustalX program ( Fig.…”

Section: Diversity Of the Heavy Chain Variable Regionmentioning

confidence: 99%

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