Limitations of the Technique to Determine Hydrogen Peroxide Levels in Exhaled Breath Condensate From Patients With Adult Respiratory Distress Syndrome

Bruhn, Alejandro; Liberona, Leonel; Lisboa, Cármen; Borzone, Gisella

doi:10.1016/s1579-2129(06)60280-2

Cited by 9 publications

(11 citation statements)

References 24 publications

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“…Oxidative stress is mainly produced by inflammatory eosinophils, macrophages and neutrophils, primarily as a defence mechanism against invading organisms. 1,2 Oxidants in EBC have been implicated in the pathophysiology of various respiratory diseases, including bronchial asthma, 3 chronic obstructive pulmonary disease, 4-6 pneumonia, 7 adult respiratory distress syndrome 8 and cystic fibrosis, 9 as well as in asymptomatic cigarette smokers. 10,11 The pathogenesis of these respiratory diseases results from the production of reactive oxygen species (ROS), which cause tissue damage and cell death.…”

Section: Introductionmentioning

confidence: 99%

“…The measurement of H2O2 in EBC provides reliable complementary data for bio-monitoring the progress of disease. Many determination methods for H2O2 in EBC have been reported, including colorimetry, 8,15 chemiluminescence, 11,16 fluorometry 17 or sensor. 18 However, due to the poor sensitivity or selectivity, these procedures need a breath condenser to collect a relatively large volume of an EBC sample for analysis, or need a special pretreatment step for a collected sample.…”

Section: Introductionmentioning

confidence: 99%

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A Sensitive Chemiluminescence Method for the Determination of H2O2 in Exhaled Breath Condensate

Zhang

Yang

2008

ANAL. SCI.

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In this paper, a novel flow injection chemiluminescence (FI-CL) method is proposed for the determination of picomolar L -1 levels of hydrogen peroxide (H2O2) in exhaled breath condensate (EBC). This method is based on the oxidation of a low concentration of luminol (10 -7 M) by H2O2 at a low concentration level (< 10 -8 M) in an alkaline medium catalyzed by a complex, K5[Cu(HIO6)2] (DPC), which is not interfered by other metal ions or horseradish peroxidase (HRP). Under the optimum conditions, H2O2 was determined over the range of 1.0 × 10 -10 to 1.0 × 10 -8 mol L -1 with a detection limit of (3σ) of 4.1 × 10 -11 mol L -1 . The relative standard deviation (RSD) was 3.2% for 5 nmol L -1 H2O2 (n = 7). The proposed method offers the advantages of ultra-sensitivity, selectivity, simplicity and rapidity for H2O2 determination. It was successfully applied to directly determine trace amounts of H2O2 (nmol L -1 ) in human's EBC of both rheum and healthy volunteers. A statistically significant difference was found between patients with rheum (n = 11) and control subjects without rheum (n = 11).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Sensitive Chemiluminescence Method for the Determination of H2O2 in Exhaled Breath Condensate

Zhang

Yang

2008

ANAL. SCI.

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“…These include H 2 O 2 (12,19, 20, 22, 23), NO (11, 13, 14, 15), leukotrienes (12, 13), 8-isoprostane (3, 12, 13, 14, 15, 18), pH (2, 11, 12, 13, 14, 25), ammonia (25), cytokines (9, 11, 16, 25, 29), protein (29), MPO (12, 15, 37), and other specific receptors. Continuous pH monitoring has successfully been performed in mechanically ventilated patients (2).…”

Section: Analysis Of Ebc Samplesmentioning

confidence: 99%

“…Diseases examined by EBC in mechanically ventilated patients include COPD (9), acute lung injury/acute respiratory distress syndrome (9, 11, 13, 16, 18, 19, 20, 22, 23, 29), pneumonia (11, 15, 16), as well as in healthy lungs of patients with concurrent illness such as brain injury (25) and elective thoracic surgery (12) (Table 4). This represents a small fraction of the possible disease states that could be examined using this method.…”

Section: Impact Of Ebc Analysis On Mechanically Ventilated Patientsmentioning

confidence: 99%

Exhaled breath condensate collection in the mechanically ventilated patient

Carter

Davis

Kovacs

2012

Respiratory Medicine

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Collection of exhaled breath condensate (EBC) is a non-invasive means of sampling the airway-lining fluid of the lungs. EBC contains numerous measurable mediators, whose analysis could change the management of patients with certain pulmonary diseases. While initially popularized in investigations involving spontaneously breathing patients, an increasing number of studies have been performed using EBC in association with mechanical ventilation. Collection of EBC in mechanically ventilated patients follows basic principles of condensation, but is influenced by multiple factors. Effective collection requires selection of a collection device, adequate minute ventilation, low cooling temperatures, and sampling times of greater than ten minutes. Condensate can be contaminated by saliva, which needs to be filtered. Dilution of samples occurs secondary to distilled water in vapors and humidification in the ventilator circuit. Dilution factors may need to be employed when investigating non-volatile biomarkers. Storage and analysis should occur promptly at −70° C to −80° C to prevent rapid degradation of samples. The purpose of this review is to examine and describe methodologies and problems of EBC collection in mechanically ventilated patients. A straightforward and safe framework has been established to investigate disease processes in this population, yet technical aspects of EBC collection still exist that prevent clinical practicality of this technology. These include a lack of standardization of procedure and analysis of biomarkers, and of normal reference ranges for mediators in healthy individuals. Once these procedural aspects have been addressed, EBC could serve as a non-invasive alternative to invasive evaluation of lungs in mechanically ventilated patients.

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“…Many different techniques have been applied to the measurement of H 2 O 2 in EBC, including fluorometry, chemiluminescence, colorimetry [6][7][8][9]. However, due to the poor sensitivity or selectivity, these procedures need a breath condenser to collect a relatively large volume of an EBC sample for analysis.…”

mentioning

confidence: 99%

Sensitive and selective chemiluminescence assay for hydrogen peroxide in exhaled breath condensate using nanoparticle-based catalysis

Zhang

Tao

et al. 2013

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Limitations of the Technique to Determine Hydrogen Peroxide Levels in Exhaled Breath Condensate From Patients With Adult Respiratory Distress Syndrome

Cited by 9 publications

References 24 publications

A Sensitive Chemiluminescence Method for the Determination of H2O2 in Exhaled Breath Condensate

A Sensitive Chemiluminescence Method for the Determination of H2O2 in Exhaled Breath Condensate

Exhaled breath condensate collection in the mechanically ventilated patient

Sensitive and selective chemiluminescence assay for hydrogen peroxide in exhaled breath condensate using nanoparticle-based catalysis

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