Limitations of pesticide genotoxicity testing using the bacterial in vitro method

Ilyushina, Nataliya A.; Egorova, Olga V.; Rakitskii, Valerii N.

doi:10.1016/j.tiv.2019.02.018

Cited by 17 publications

(3 citation statements)

References 13 publications

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“…Therefore, different biomarkers are used to evaluate the genotoxicity of pesticides on organisms. 26 Previous researchers have preferred micronucleus assay, sister chromatids exchange, chromosomal aberration, comet assay, and gamma H2AX assay to assess genotoxicity in vitro and in vivo. [27][28][29] Micronucleus and comet assays are commonly used to evaluate pesticide genotoxicity on different models.…”

Section: Discussionmentioning

confidence: 99%

The possible cytotoxicity and genotoxicity assessment of indaziflam on HepG2 cells

Könen-Adıgüzel

2023

Hum Exp Toxicol

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The use of pesticides in farmland has increased considerably to protect crops against pests, weeds, and diseases. However, pesticides and/or their residues in ecosystems may affect non-target organisms. Indaziflam is a widely used herbicide in agricultural areas in the southern region of Turkey. Therefore, this study aimed to investigate the possible genotoxic and cytotoxic effects of indaziflam on HepG2 cells using comet assay, micronucleus assay, and xCELLigence. The HepG2 cells were treated with various concentrations of indaziflam for different duration of time based on xCELLigence results. Accordingly, the cells were incubated with indaziflam at final concentrations of 1, 5, 10, 20, 40, and 80 μg/mL for 96 h for cytotoxicity assay. To assess genotoxicity, cells were treated with indaziflam at final concentrations of 10, 40, and 100 μg/mL for 4 and 24 h. Ethanol was used as a solvent for indaziflam. Hydrogen peroxide (40 μM) was used as a positive control. Studies have revealed that indaziflam did not show a statistically cytotoxic effect at the tested doses. Nevertheless, genotoxicity studies showed that indaziflam induced both DNA strand breaks and micronucleus numbers depending on the exposure time and dose.

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Section: Discussionmentioning

confidence: 99%

The possible cytotoxicity and genotoxicity assessment of indaziflam on HepG2 cells

Könen-Adıgüzel

2023

Hum Exp Toxicol

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“…TGAIs imidacloprid, imazalil and tebuconazole, separately and in combination, were studied using Ames test ( Salmonella typhimurium) according to OECD Guideline № 471 [ 26 ]. The plate incorporation method both in the absence and in the presence of an exogenous metabolic activation system (S9) was used as described in one of our previous publications [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Genotoxicity of mixture of imidacloprid, imazalil and tebuconazole

et al. 2020

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“…However, PCAs can be easily released into environmental media such as water and soil during the application, resulting in a large area of pollution. Various studies have proved that residual PCAs can do harm to humans and organisms [2][3][4]. To protect human health, the maximum contaminant levels have been regulated in several countries and organizations [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Development of monolith/aminated carbon nanotubes composite‐based solid‐phase microextraction of phenoxycarboxylic acids herbicides in water and soil samples

Chen

Luo

Huang

2021

J of Separation Science

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In this study, a new adsorbent based on monolith/aminated carbon nanotubes composite was facilely prepared and employed as the extraction phase of multiple monolithic fibers solid-phase microextraction for the capture of phenoxycarboxylic acids herbicides. The adsorbent was fabricated by mingling aminated carbon nanotubes in the poly (allylthiourea-co-ethylene glycol dimethacrylate) monolith. Various techniques were employed to characterize the morphology, structure, and pore size of the prepared adsorbent. The proposed microextraction method displayed satisfactory capture performance towards studied analytes through multi-interactions such as hydrogen-bonding, hydrophobic and π-π interactions. Under the optimized conditions, a sensitive and reliable method to quantify trace analytes in water and soil samples was developed. The limits of detection were in the ranges of 0.13-0.25 μg/L and 0.20-0.61 μg/kg for water and soil samples, respectively. The practicality of the introduced method was demonstrated by applying it to monitor the contents of studied analytes in environmental water and soil samples. Satisfactory fortified recoveries (76.4-119%) and reproducibility were obtained. The achieved results well demonstrated that the suggested microextraction technique can efficiently extract phenoxycarboxylic acids and the developed method exhibits a promising potential for reliable and sensitive quantification of trace analytes in complex samples.

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Limitations of pesticide genotoxicity testing using the bacterial in vitro method

Cited by 17 publications

References 13 publications

The possible cytotoxicity and genotoxicity assessment of indaziflam on HepG2 cells

The possible cytotoxicity and genotoxicity assessment of indaziflam on HepG2 cells

Genotoxicity of mixture of imidacloprid, imazalil and tebuconazole

Development of monolith/aminated carbon nanotubes composite‐based solid‐phase microextraction of phenoxycarboxylic acids herbicides in water and soil samples

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