Limitations of morphological ploidy estimation methods in Fragaria

Chambers, Alan H.; Pollard, Heather; Folta, Kevin M.

doi:10.3233/jbr-130057

Cited by 5 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A linear x--axis scale was used at this point and focused to clearly visualize the cell cycle distribution and graphically evaluate the fit. Flow cytometry on strawberry tissues was performed as described [37].…”

Section: Protoplast Isolation and Flow Cytometry Analysismentioning

confidence: 99%

Analysis of Block of cell proliferation 1 (BOP1) activity in strawberry and Arabidopsis

et al. 2016

Self Cite

View full text Add to dashboard Cite

Block of cell proliferation (BOP) proteins are conserved among eukaryotes, and studies in mammals and yeast have described their role in ribosome biogenesis and cell cycle regulation. A BOP1 orthologue was identified in plants, and loss-of-function analyses in tobacco cells confirmed similar activities. This report characterizes a role for BOP1 activity in planta. Two transgenic plant species were used: the diploid strawberry (Fragaria vesca) and Arabidopsis thaliana. FvBOP1 silencing showed changes in pre-rRNA processing, and demonstrated FvBOP1's role in growth and physiology throughout different stages of plant development. In the strawberry, repression of FvBOP1 activity decreased plant fitness prior to flowering, followed by plant death after the reproductive transition, indicating that BOP1 activity is required for transition back to vegetative growth after flowering. A T-DNA null allele of the AtBOP1 gene is lethal, and a 50% decrease in transcript accumulation is sufficient to cause severe developmental defects linked to defective cell division. The conserved protein BOP1 is essential for viability. Lower transcript levels result in defects in rRNA processing and developmental abnormalities that are consistent with its predicted role in ribosome biogenesis.

show abstract

Section: Protoplast Isolation and Flow Cytometry Analysismentioning

confidence: 99%

Analysis of Block of cell proliferation 1 (BOP1) activity in strawberry and Arabidopsis

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several methods have been used to estimate ploidy levels in crops [21], the most common among which are chromosome counting and flow cytometry. The flow cytometry technique has been used for determining the ploidy levels of various plant species, including yams [15,[22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

et al. 2021

View full text Add to dashboard Cite

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization.

show abstract

“…Several methods have been used to estimate ploidy levels in crops [19], the most common among which are chromosome counting and flow cytometry. The flow cytometry technique has been used for determining the ploidy levels of various plant species, including yams.…”

Section: Introductionmentioning

confidence: 99%

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

Gatarira¹,

Němečková²,

Šimoníková³

et al. 2021

Preprint

View full text Add to dashboard Cite

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated staple supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and inter-specific hybridization.

show abstract

Limitations of morphological ploidy estimation methods in Fragaria

Cited by 5 publications

References 25 publications

Analysis of Block of cell proliferation 1 (BOP1) activity in strawberry and Arabidopsis

Analysis of Block of cell proliferation 1 (BOP1) activity in strawberry and Arabidopsis

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

Contact Info

Product

Resources

About