Limitations of flow cytometry for the specific detection of bacteria in mixed populations

Phillips, A.P.; Martin, Keith L.

doi:10.1016/0022-1759(88)90278-5

Cited by 38 publications

(17 citation statements)

References 10 publications

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“…Because B. anthracis spores are not permeable to nucleic acid probes, the spores can only be labeled using antibodies to spore surface proteins. Early attempts of using flow cytometry to detect B. anthracis took several hours to complete [51,52]. By reducing the reaction time of the antibody with spores and eliminating washing steps, Stopa developed an assay that took 5 min and detected as low as 1000 spores/ml [53].…”

Section: Flow Cytometrymentioning

confidence: 99%

Recent advances in the rapid detection of Bacillus anthracis

Levine

Tang

Pei

2005

Reviews in Medical Microbiology

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Bacillus anthracis is a Gram-positive, spore-forming rod that causes anthrax. Culturebased methods are the gold standard for the identification of virulent B. anthracis strains but these require days for completion. The experience from the anthrax attacks in September and October of 2001 revealed the urgent need for methods that can rapidly detect this pathogen with high reliability. Because of the extensive homology among non-anthrax Bacillus sp. at the chromosomal level, rapid detection of virulent B. anthracis strains depends on markers associated with the two plasmids, pXO1 and pXO2, responsible for its virulence. Genes encoding toxins and capsules have been used as markers for pXO1 and pXO2, respectively, in methods that are designed for rapid and sensitive detection of B. anthracis DNA, such as real-time polymerase chain reaction, direct liquid phase hybridization, and DNA microarrays. A variety of platforms can be modified to suit the needs for rapid detection of B. anthracis antigens, but little is known about plasmid-encoded antigens expressed in spores. Future studies should be aimed at detecting markers for pXO1 and pXO2in viable spores. ß 2005 Lippincott Williams & Wilkins Reviews in Medical Microbiology 2005, 16:125-133

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Section: Flow Cytometrymentioning

confidence: 99%

Recent advances in the rapid detection of Bacillus anthracis

Levine

Tang

Pei

2005

Reviews in Medical Microbiology

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“…in 25 g of product, which corresponds to a sterility test. Optoelectronic noise generated at the instrument settings needed to detect small particles such as immunoXuorescent bacteria as well as interferences from particles contained in the product which produce the same Xuorescence as labelled bacteria are both likely to prevent the direct detection of such low bacterial concentrations [69]. Thus, in these circumstances, ampliWcation of bacterial concentrations by culture is likely to be necessary before FCM analysis.…”

Section: Spoilage and Pathogen Microorganism Contaminationmentioning

confidence: 98%

Flow cytometry applications in the food industry

Comas-Riu

Rius

2009

J Ind Microbiol Biotechnol

101

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Flow cytometry has become a valuable tool in food microbiology. By analysing large numbers of cells individually using light-scattering and fluorescence measurements, this technique reveals both cellular characteristics and the levels of cellular components. Flow cytometry has been developed to rapidly enumerate microorganisms; to distinguish between viable, metabolically active and dead cells, which is of great importance in food development and food spoilage; and to detect specific pathogenic microorganisms by conjugating antibodies with fluorochromes, which is of great use in the food industry. In addition, high-speed multiparametric data acquisition, analysis and cell sorting, which allow other characteristics of individual cells to be studied, have increased the interest of food microbiologists in this technique. This mini-review gives an overview of the principles of flow cytometry and examples of the application of this technique in the food industry.

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“…In the recent past, use of flow cytometry for bacterial characterisation has relied upon immuno-fluorescence-based methods [11][12][13][14]16 . Flow cytometry provides a rapid and precise method for the detection, enumeration, and identification of the bacteria.…”

Section: Introductionmentioning

confidence: 99%

Flow-cytometric Analysis of Bacillus anthracis Spores

Kamboj

Agarwal

Dwarakanath

et al. 2006

DSJ

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Flow-cytometric technique has been established as a powerful tool for detection and identification of microbiological agents. Unambiguous and rapid detection of Bacillus anthracis spores has been reported using immunoglobulin G-fluorescein isothiocyanate conjugate against live spores. In addition to the high sensitivity, the present technique could differentiate between spores of closely related species, eg, Bacillus cereus and Bacillus subtilis using fluorescence intensity. The technique can be used for detection of live as well as inactivated spores making it more congenial for screening of suspected samples of bioterrorism.

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Limitations of flow cytometry for the specific detection of bacteria in mixed populations

Cited by 38 publications

References 10 publications

Recent advances in the rapid detection of Bacillus anthracis

Recent advances in the rapid detection of Bacillus anthracis

Flow cytometry applications in the food industry

Flow-cytometric Analysis of Bacillus anthracis Spores

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