Limit of change in unsaturation index of fatty acid composition of phospholipids at adaptation of molluscs to biogenic and abiogenic environmental factors

Chebotareva, M. A.; Забелинский, С. А.; Shukolyukova, E. P.; Кривченко, А. И.

doi:10.1134/s0022093011050069

Cited by 5 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, fatty acid profiles in flesh samples did not differ between the initial and reference groups (data not shown) and among the treatments. Similarly, fatty acid profiles of total phospholipids in foot tissues of M. edulis individuals, previously adapted to different temperatures, were shown to remain constant (Chebotareva, Zabelinskii, Shukolyukova & Krivchenko ). In fish, temperature did not have significant impact on fatty acid composition in muscle of juvenile Pacific cod Gadus microcephalus and walleye Pollock Theragra chalcogramma over a 2‐month period (Copeman, Laurel & Parrish ) and juvenile black bream Acanthopagrus buthcheri (Elsdon ).…”

Section: Discussionmentioning

confidence: 97%

“…Similarly, fatty acid profiles of total phospholipids in foot tissues of M. edulis individuals, previously adapted to different temperatures, were shown to remain constant (Chebotareva, Zabelinskii, Shukolyukova & Krivchenko 2011). However, the effect of temperature on biochemical processes, such as fatty acid storage and utilization, is not well understood.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biochemical composition of New Zealand geoduck clam broodstock (Panopea zelandica ) conditioned under different temperature and feeding regimes

Pook

Ragg

et al. 2016

Aquac Res

View full text Add to dashboard Cite

Geoduck clams are amongst the most valuable cultured shellfish available on the world market, driving interest in the establishment of the native New Zealand species, Panopea zelandica (Quoy & Gaimard, 1835), as a candidate for aquaculture. A critical element of domestication is the effective management of breeding stock to optimize gamete and offspring quality. To develop a reliable broodstock conditioning protocol for P. zelandica, we investigated the biochemical composition of geoducks exposed to nine factorial treatment combinations of temperature (7.5, 11.5 and 16.5°C) and feeding ration [10 000, 50 000 and 100 000 cells mL À1 of a (1:1 cell count) mixture of Tisochrysis lutea and Chaetoceros muelleri]. In addition, a reference group of geoducks was conditioned in an aquaculture pond-nursery system, providing a dilute, mixed phytoplankton culture at ambient temperature. Glycogen, protein, lipid and fatty acid contents of flesh and viscera were measured at days 0, 36 and 73 of conditioning. Glycogen, protein and lipid analyses indicated that geoducks within all treatments achieved a positive energy balance, except for those in the treatment combining the highest temperature and lowest feeding ration. Comparisons of fatty acid profiles of animals among treatments and with the reference group revealed that eicosapentaenoic (EPA, C20:5n-3), docosahexaenoic (DHA, C22:6n-3) and arachidonic (ARA, C20:4n-6) fatty acids were important contributors to gametogenic development for geoduck conditioning.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Biochemical composition of New Zealand geoduck clam broodstock (Panopea zelandica ) conditioned under different temperature and feeding regimes

Pook

Ragg

et al. 2016

Aquac Res

View full text Add to dashboard Cite

show abstract

“…Fatty acids are prominent and essential components of phospholipids and sphingolipids that constitute the plasma membrane and the membranes of various organelles [8], [9]. Furthermore, they also play important roles in cell signaling and energy storage through the formation of triglycerides [10].…”

Section: Introductionmentioning

confidence: 99%

3-Oxoacyl-ACP Reductase from Schistosoma japonicum: Integrated In Silico-In Vitro Strategy for Discovering Antischistosomal Lead Compounds

et al. 2013

View full text Add to dashboard Cite

BackgroundSchistosomiasis is a disease caused by parasitic worms and more than 200 million people are infected worldwide. The emergence of resistance to the most commonly used drug, praziquantel (PZQ), makes the development of novel drugs an urgent task. 3-oxoacyl-ACP reductase (OAR), a key enzyme involved in the fatty acid synthesis pathway, has been identified as a potential drug target against many pathogenic organisms. However, no research on Schistosoma japonicum OAR (SjOAR) has been reported. The characterization of the SjOAR protein will provide new strategies for screening antischistosomal drugs that target SjOAR.Methodology/Principal FindingsAfter cloning the SjOAR gene, recombinant SjOAR protein was purified and assayed for enzymatic activity. The tertiary structure of SjOAR was obtained by homology modeling and 27 inhibitor candidates were identified from 14,400 compounds through molecular docking based on the structure. All of these compounds were confirmed to be able to bind to the SjOAR protein by BIAcore analysis. Two compounds exhibited strong antischistosomal activity and inhibitory effects on the enzymatic activity of SjOAR. In contrast, these two compounds showed relatively low toxicity towards host cells.Conclusions/SignificanceThe work presented here shows the feasibility of isolation of new antischistosomal compounds using a combination of virtual screening and experimental validation. Based on this strategy, we successfully identified 2 compounds that target SjOAR with strong antischistosomal activity but relatively low cytotoxicity to host cells.

show abstract

Fatty Acid Composition of Gonads and Gametes in the Black Sea Bivalve Mollusk Mytilus galloprovincialis Lam. at Different Stages of Sexual Maturation

Kapranova

Nekhoroshev

Malakhova

et al. 2019

J Evol Biochem Phys

View full text Add to dashboard Cite

Limit of change in unsaturation index of fatty acid composition of phospholipids at adaptation of molluscs to biogenic and abiogenic environmental factors

Cited by 5 publications

References 8 publications

Biochemical composition of New Zealand geoduck clam broodstock (Panopea zelandica ) conditioned under different temperature and feeding regimes

Biochemical composition of New Zealand geoduck clam broodstock (Panopea zelandica ) conditioned under different temperature and feeding regimes

3-Oxoacyl-ACP Reductase from Schistosoma japonicum: Integrated In Silico-In Vitro Strategy for Discovering Antischistosomal Lead Compounds

Fatty Acid Composition of Gonads and Gametes in the Black Sea Bivalve Mollusk Mytilus galloprovincialis Lam. at Different Stages of Sexual Maturation

Contact Info

Product

Resources

About