“…(Victorica and Galvan 2003) evaluated the efficiency of recovery of this technique and obtained 90 % for helminth eggs in samples of influent, effluent and sludge. The USEPA determined that the detection limit is 1 viable helminth egg per 4 g of sewage sludge (dry weight) (Capizzi-Banas et al 2004). In this study, we choose not to use the method described by USEPA (USEPA 1999), for checking the viability recommended as this includes the incubation of the biosolid during three or 4 weeks and this is biased by fungi proliferation, while the Mexican Official Norm did not contemplate such a step, which is of fundamental importance in the epidemiology of parasitic infections (Victorica and Galvan 2003).…”
Biosolid is the product of the activated sludge treatment system and its final disposition is subject of ongoing discussion as this residue can therefore harbor a great number and variety of pathogens. This study was aimed to (1) monitor the presence of Giardia and Cryptosporidium in biosolid samples from a treatment plant in Campinas, SP, Brazil, (2) observe Giardia cyst wall morphological integrity in treated samples using scanning electron microscopy (SEM) and (3) verify the presence and viability of helminth eggs. Cysts were present in 33.3 % of the samples, whereas oocysts were detected in 8.3 %. Viable Ascaris sp. Toxocara sp. and similar to Trichuris sp. eggs were found through the use of Mexican Official Norm. Results demonstrate the difficulties inherent in working with biosolid as factors such as temperature, ionic strength and pH influenced the recovery of cysts and oocysts. Pores and ruptures were not observed in cyst wall visualized by SEM following 45 days of exposure to sunlight, only minimal morphological changes. These observations emphasize both the importance of adequate treatment of sewage sludge and the need to develop appropriate techniques for the detection of Giardia and Cryptosporidium in this type of sample. This is the first time that a study was done in a real scale for biosolid samples in determining the presence of pathogenic protozoa as Giardia and Cryptosporidium in Brazil, and also observed minimal cyst wall damage after sunlight treatment.
“…(Victorica and Galvan 2003) evaluated the efficiency of recovery of this technique and obtained 90 % for helminth eggs in samples of influent, effluent and sludge. The USEPA determined that the detection limit is 1 viable helminth egg per 4 g of sewage sludge (dry weight) (Capizzi-Banas et al 2004). In this study, we choose not to use the method described by USEPA (USEPA 1999), for checking the viability recommended as this includes the incubation of the biosolid during three or 4 weeks and this is biased by fungi proliferation, while the Mexican Official Norm did not contemplate such a step, which is of fundamental importance in the epidemiology of parasitic infections (Victorica and Galvan 2003).…”
Biosolid is the product of the activated sludge treatment system and its final disposition is subject of ongoing discussion as this residue can therefore harbor a great number and variety of pathogens. This study was aimed to (1) monitor the presence of Giardia and Cryptosporidium in biosolid samples from a treatment plant in Campinas, SP, Brazil, (2) observe Giardia cyst wall morphological integrity in treated samples using scanning electron microscopy (SEM) and (3) verify the presence and viability of helminth eggs. Cysts were present in 33.3 % of the samples, whereas oocysts were detected in 8.3 %. Viable Ascaris sp. Toxocara sp. and similar to Trichuris sp. eggs were found through the use of Mexican Official Norm. Results demonstrate the difficulties inherent in working with biosolid as factors such as temperature, ionic strength and pH influenced the recovery of cysts and oocysts. Pores and ruptures were not observed in cyst wall visualized by SEM following 45 days of exposure to sunlight, only minimal morphological changes. These observations emphasize both the importance of adequate treatment of sewage sludge and the need to develop appropriate techniques for the detection of Giardia and Cryptosporidium in this type of sample. This is the first time that a study was done in a real scale for biosolid samples in determining the presence of pathogenic protozoa as Giardia and Cryptosporidium in Brazil, and also observed minimal cyst wall damage after sunlight treatment.
“…Major risk in agriculture is especially where untreated wastewater and excreta are used and sanitation standards are low (Capizzi-Banas et al, 2004;Kone et al, 2007). Hence, guidelines or standards on the hygienic quality of the biosolids or faecal sludge intended for agriculture use must be required.…”
SummaryContamination of soil with helminth eggs in the samples of fields, kitchen gardens, yards and composts in rural areas of Lodz district (Poland) was investigated. In this study, helminth eggs were found in 60 -100 % of field samples, in 20 -100 % of yards samples, in 0 -20 % of kitchen gardens samples and in 10 -100 % of composts. The highest average density of helminth eggs in 100 g of soil was detected in composts (44.0), then fields (28.5) and yards (18.0). In samples taken from kitchen gardens the average density of eggs was 0.4/100/g of soil. The comparison of frequency of positive samples from fields, kitchen gardens and yards did not exhibit significant difference (p > 0.05). The soil samples of fields contained mainly eggs of Ascaris spp. (87.7 %), less frequently Toxocara spp. (7.7 %) and Trichuris spp. (3.5 %). In samples from yards among detected eggs the most often were Toxocara spp. (73.9 %), and there were statistically significant differences in comparison with fields (7.7 %) and composts (0.3 %). The highest prevalence of eggs with moving larva was noted in yards (25.6 %), which differ statistically significantly from analogous value for fields (p < 0.05) and composts (p < 0.0001). These results showed a considerable infestation of soil with geohelminth eggs of the examined rural areas of Lodz district which is a potential source of antropozoonosis.
“…then, the spatula was used to till the top few cm of the soil surface to blend in the ova-biosolid mixture. additional deionized (di) water was added to the biosolid-amended soil to increase the moisture content of each microcosm to approximately 22.25% (less than 25% suggested for pathogen destruction) [12,34]. a total of 48 microcosms were prepared.…”
This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.
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