Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

Abstract

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

Uji Potensi Antibakteri Ekstrak Kulit Jeruk Nipis Citrus aurantifolia (Christm) Swingle Terhadap Vibrio sp.

“…Penentuan kategori hambatan dilakukan berdasarkan Greenwood, et al (2007) Ekstrak kulit jeruk nipis juga menunjukkan aktivitas antibakteri dalam menghambat pertumbuhan Staphylococcus aureus dengan menggunakan konsenteasi 80% ekstrak (Ekawati, et al, 2019). Pada konsentrasi 100%, ekstrak kulit jeruk nipis juga mampu menghambat pertumbuhan Escherichia coli dengan luas zona hambat yang ditunjukkan sebesar 26,69 mm (Sari, et al, 2021). Konsentrasi 50% ekstrak kulit jeruk nipis mampu menghasilkan zona hambat terhadap pertumbuhan Shigella dysentriae sebesar 33,5 mm (Sari, et al, 2022).…”

Atsiri Oil Potential of Peel of Kaffir Lime, Lime, and Calamansi Orange as Antioxidant and Antibacteria

“…Environment can be produced while maintaining environmental sustainability. One of the environmental wastes that will be used as a product that has a fairly high selling value and is friendly to the environment is orange peel waste (Sari et al, 2021). Orange peels are abundant, easy to obtain and their utilization is not maximized, making this waste suitable as an active ingredient in anti-aging cosmetics.…”