Lima bean nodulates efficiently with Bradyrhizobium strains isolated from diverse legume species

Costa, Elaine Martins da; Ribeiro, Paula Rose de Almeida; Lima, Wellington de; Farias, Thiago Palhares; Moreira, Fátima Maria de Souza

doi:10.1007/s13199-017-0473-8

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…where strains varied in symbiotic effectiveness and only few were superior in N 2 fixation. At a global scale, studies on different legumes with indigenous strains have also shown varying effectiveness in symbiosis (da Costa et al 2017;de Almeida Ribeiro et al 2015;Thrall et al 2011;Xu et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya

Ndungu

Messmer

Ziegler³

et al. 2018

Appl Microbiol Biotechnol

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Cowpea N fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a field trial in Mbeere during the 2014 short rains. Strains from bacteroids of cowpea nodules from pot and field experiments were accurately identified as Bradyrhizobium by MALDI-TOF based on the SARAMIS™ database. In the field, abundant indigenous populations 7.10 × 10 rhizobia g soil, outcompeted introduced strains. As revealed by MALDI-TOF, indigenous strains clustered into six distinct groups (I, II, III, IV, V and VI), group III were most abundant occupying 80% of nodules analyzed. MALDI-TOF was rapid, affordable and reliable to identify Bradyrhizobium strains directly from nodule suspensions in competition pot assays and in the field with abundant indigenous strains thus, its suitability for future competition assays. Evaluating strain competitiveness and then symbiotic efficacy is proposed in bioprospecting for potential cowpea inoculant strains.

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Section: Discussionmentioning

confidence: 99%

Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya

Ndungu

Messmer

Ziegler³

et al. 2018

Appl Microbiol Biotechnol

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“…NN and NDM are important variables for selecting rhizobia because they reflect the competitive capacity and symbiotic efficiency of the strains. Nodulation data, especially NDM, are usually positively correlated with the fixation of N 2 and N accumulation in legumes (Costa et al, 2017a;Sulieman et al, 2019). However, it should be noted that good nodulation does not always reflect the efficiency of the strains because several edaphoclimatic factors can interfere with the survival and efficiency of these microorganisms (Moreira et al, 2010;Kaschuk et al, 2016;Castro et al, 2017).…”

Section: /8mentioning

confidence: 99%

“…Soil factors, besides the texture and content of organic matter, which differed between soils (Table 1), could influence the performance of the strains in the production of SDM (Moreira et al, 2010;Soares et al, 2014;Costa et al, 2017a). However, better adaptation of the soybean cultivars in the Oxisol should also be considered because even the non-inoculated treatments presented higher production of SDM in this soil.…”

Section: /8mentioning

confidence: 99%

Bradyrhizobium brasilense as an efficient soybean microsymbiont in two contrasting soils of the southwestern region of Piauí (Cerrado biome)

Rocha

Costa

et al. 2021

Agraria

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The goal of this study was to evaluate the efficiency of Bradyrhizobium brasilense strains native to the soil of the semiarid region in northeastern Brazil in symbiosis with soybeans in two contrasting soils in southwestern Piauí. A pot experiment was conducted using a randomized block design in a 10 × 2 factorial scheme. There were 10 nitrogen (N) sources: six native Bradyrhizobium strains [UFLA06-13, UFLA06-15, UFLA06-19, UFLA06-21, UFLA06-22 (B. brasilense), and UFLA06-24 (Bradyrhizobium sp.)], two controls with strain SEMIA 5019 (B. elkanii) and a commercial inoculant [SEMIA 5079 (B. japonicum) + SEMIA 5080 (B. diazoefficiens)] recommended for soybeans, and two controls without the application of an inoculant (one with and the other without the application of mineral N). The second experimental factor corresponded to the use of two soils (Oxisol and Quartzarenic Neosol). All strains showed increased nodulation and shoot nitrogen content in soybean plants in both soils. Most strains promoted higher nitrogen fixation when inoculated in the Oxisol. UFLA06-19, UFLA06-22, and UFLA06-24 were efficient in nitrogen accumulation in the shoots of soybeans in the Oxisol. This is the first report regarding the efficiency of B. brasilense strains in symbiosis with soybeans under different soil conditions.

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“…The lima bean (Phaseolus lunatus L.), is the second most important domesticated species of Phaseoulus globally and has significant potential as a valuable alternative resource for food security, quality, and sustainability in the face of climate change [21]. Its symbiotic relationship with rhizobia microorganisms offers an attractive strategy for soil fertility restoration, soil health and quality improvement, and sustainable agricultural practices [22]. This legume crop is known to establish symbiotic relationships with nitrogen-fixing rhizobia bacteria, mainly of the Bradyrhizobium genus, which can contribute to soil fertility and ecosystem sustainability [22,23].…”

Section: Introductionmentioning

confidence: 99%

“…Its symbiotic relationship with rhizobia microorganisms offers an attractive strategy for soil fertility restoration, soil health and quality improvement, and sustainable agricultural practices [22]. This legume crop is known to establish symbiotic relationships with nitrogen-fixing rhizobia bacteria, mainly of the Bradyrhizobium genus, which can contribute to soil fertility and ecosystem sustainability [22,23]. It possesses distinctive attributes that contribute to its role as a soil quality restorer, particularly its capacity to enrich the soil with readily available nitrogen [24], reducing the dependence on synthetic fertilizers and its subsequent ecological consequences [25].…”

Section: Introductionmentioning

confidence: 99%

Reactivating the Potential of Lima Bean (Phaseolus lunatus) for Enhancing Soil Quality and Sustainable Soil Ecosystem Stability

Abdulraheem,

Moshood,

et al. 2024

Agriculture

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Background: This study explores the role of leguminous crops like lima bean in enhancing soil quality and ecosystem stability. Despite existing studies on agronomic aspects, there is a significant research gap on its impact on soil organic matter level, microbial activity, soil health, and nutrient availability. Therefore, this study examines the capacity of lima bean to reactivate soil quality, focusing on its impact on soil organic matter level, microbial activity, soil health, and nutrient availability. Methods: The experimental area was set up in 2023 using three replicates and a randomized block design. Two treatments were used: lima bean-planted plots and control plots with various weeds and without lima bean. Post-harvest soil samples were collected from various agroecological zones and sterilely packed, and physical, chemical, and biological indices were examined. Results: lima bean significantly affected nutrients, enzymes, soil microbial respiration, and other markers. Amylase activity (0.41**) was positively correlated with urease activity (0.73**), while dehydrogenase activity positively correlated with both. Dehydrogenase activity was negatively correlated with total nitrogen (0.66**) and sulfur (0.60**). Lima bean significantly affected soil quality, with all locations showing higher ratings (55–77%) than wild land, except for location D (Ilora). A total of 70% of total nitrogen variation may be attributed to soil quality (r2 = 0.696). Lima bean enhanced soil quality, potentially enhancing productivity and reducing dependence on inorganic nitrogen inputs. Conclusions: The symbiotic relationship between lima bean and nitrogen-fixing bacteria improves nutrient cycling, enhancing agricultural productivity and environmental conservation. Future research should explore the economic viability of integrating lima bean into crop rotations or agroforestry systems for sustainable agricultural practices, providing valuable information for farmers.

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Lima bean nodulates efficiently with Bradyrhizobium strains isolated from diverse legume species

Cited by 8 publications

References 37 publications

Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya

Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya

Bradyrhizobium brasilense as an efficient soybean microsymbiont in two contrasting soils of the southwestern region of Piauí (Cerrado biome)

Reactivating the Potential of Lima Bean (Phaseolus lunatus) for Enhancing Soil Quality and Sustainable Soil Ecosystem Stability

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