LIM homeobox protein 5 (Lhx5) is essential for mamillary body development

Miquelajáuregui, Amaya; Sandoval-Schaefer, Teresa; Martínez-Armenta, Miriam; Pérez-Martı́nez, Leonor; Cárabez, A.; Zhao, Yangu; Heide, Michael; Álvarez-Bolado, Gonzalo; Varela‐Echavarría, Alfredo

doi:10.3389/fnana.2015.00136

Cited by 11 publications

(5 citation statements)

References 61 publications

(126 reference statements)

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“…For instance, Lhx5 , a gene expressed in NP cells when contacting DE, is known to promote forebrain development by regulating the Wnt signaling pathway ( 33 ). Knockdown of Lhx5 resulted in apoptotic hypothalamic development ( 49 ). Nkx2-1, another gene expressed in NP cells when contacting DE, is also recognized for its role in response to dorsal–ventral patterning in the neural tube and for specifying cortical interneuron subtypes ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

Kim

Rothová

Madan

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Development of multicellular organisms is orchestrated by persistent cell–cell communication between neighboring partners. Direct interaction between different cell types can induce molecular signals that dictate lineage specification and cell fate decisions. Current single-cell RNA-seq technology cannot adequately analyze cell–cell contact-dependent gene expression, mainly due to the loss of spatial information. To overcome this obstacle and resolve cell–cell contact-specific gene expression during embryogenesis, we performed RNA sequencing of physically interacting cells (PIC-seq) and assessed them alongside similar single-cell transcriptomes derived from developing mouse embryos between embryonic day (E) 7.5 and E9.5. Analysis of the PIC-seq data identified gene expression signatures that were dependent on the presence of specific neighboring cell types. Our computational predictions, validated experimentally, demonstrated that neural progenitor (NP) cells upregulate Lhx5 and Nkx2-1 genes, when exclusively interacting with definitive endoderm (DE) cells. Moreover, there was a reciprocal impact on the transcriptome of DE cells, as they tend to upregulate Rax and Gsc when in contact with NP cells. Using individual cell transcriptome data, we formulated a means of computationally predicting the impact of one cell type on the transcriptome of its neighboring cell types. We have further developed a distinctive spatial-t-distributed stochastic neighboring embedding to display the pseudospatial distribution of cells in a 2-dimensional space. In summary, we describe an innovative approach to study contact-specific gene regulation during embryogenesis.

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Section: Discussionmentioning

confidence: 99%

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

Kim

Rothová

Madan

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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“…It is noteworthy that, at stage 32 (present results), the NLT is negative for the markers considered here, although it is positive for ScLhx5 at previous stages ( Santos-Durán et al, 2018 ). Similarly, the mammalian ventromedial nucleus seems to be positive for Lhx5 and abuts Dlx -expressing territories at early stages ( Sheng et al, 1997 ; Abellán et al, 2010 ), but it is negative at later stages ( Szabó et al, 2009 ; Heide et al, 2015 ; Miquelajáuregui et al, 2015 ). The catshark NLT presents several types of peptidergic cells (somatostatin, Sobrido-Cameán et al, 2020 ; met-enkephalin and leu-enkephalin, Vallarino et al, 1994 ; melanin-concentrating hormone, Vallarino et al, 1989a ; atrial natriuretic factor, Vallarino et al, 1990b ; delta sleep-inducing peptide, Vallarino et al, 1992 ; beta-endorphin, Vallarino et al, 1989b ; galanin, Vallarino et al, 1991 ; neuropeptide Y, Vallarino et al, 1988 ; corticotropin-releasing factor, Vallarino et al, 1989c ; adrenocorticotropic hormone, Vallarino and Ottonello, 1987 , Vallarino et al, 1989b ), and is thought to be associated with the hypophysis, as reported in actinopterygian fishes (reviewed in Butler and Hodos, 2005 ).…”

Section: Discussionmentioning

confidence: 97%

Developmental genoarchitectonics as a key tool to interpret the mature anatomy of the chondrichthyan hypothalamus according to the prosomeric model

Santos-Durán

Ferreiro-Galve

Mazan³

et al. 2022

Front. Neuroanat.

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The hypothalamus is a key vertebrate brain region involved in survival and physiological functions. Understanding hypothalamic organization and evolution is important to deciphering many aspects of vertebrate biology. Recent comparative studies based on gene expression patterns have proposed the existence of hypothalamic histogenetic domains (paraventricular, TPa/PPa; subparaventricular, TSPa/PSPa; tuberal, Tu/RTu; perimamillary, PM/PRM; and mamillary, MM/RM), revealing conserved evolutionary trends. To shed light on the functional relevance of these histogenetic domains, this work aims to interpret the location of developed cell groups according to the prosomeric model in the hypothalamus of the catshark Scyliorhinus canicula, a representative of Chondrichthyans (the sister group of Osteichthyes, at the base of the gnathostome lineage). To this end, we review in detail the expression patterns of ScOtp, ScDlx2, and ScPitx2, as well as Pax6-immunoreactivity in embryos at stage 32, when the morphology of the adult catshark hypothalamus is already organized. We also propose homologies with mammals when possible. This study provides a comprehensive tool to better understand previous and novel data on hypothalamic development and evolution.

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“…For instance, Lhx5 , a gene expressed in NP cells when contacting DE, is known to promote forebrain development by regulating the Wnt signaling pathway (32). Knockdown of Lhx5 resulted in apoptotic hypothalamic development (46). Nkx2-1 , another gene expressed in NP cells when contacting DE, is also recognized for its role in response to dorsal-ventral patterning in the neural tube and for specifying cortical interneuron subtypes (33).…”

Section: Discussionmentioning

confidence: 99%

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

Kim

Rothov

Liao

et al. 2021

Preprint

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Cells continuously communicate with the neighboring cells during development. Direct interaction of different cell types can induce molecular signals dictating lineage specification and cell fate decisions. The current single cell RNAseq (scRNAseq) technology cannot study cell contact dependent gene expression due to the loss of spatial information. To overcome this issue and determine cell contact specific gene expression during embryogenesis, we performed RNA sequencing of physically interacting cells (PICseq) and assessed alongside our single cell transcriptomes (scRNAseq) derived from developing mouse embryos between embryonic day (E) 7.5 and E9.5. Analysis of PICseq data identifies an interesting suite of gene expression signatures depending on neighboring cell types. For instance, neural progenitor (NP) cells expressed Nkx2-1 when interacting with definitive endoderm (DE) and DE cells expressed Gsc when interacting with NP. Based on the identified cell contact specific genes, we devised a means to predict the neighboring cell types from individual cell transcriptome. We further developed spatial-tSNE to show the pseudo-spatial distribution of cells in a 2-dimensional space. In sum, we suggest an approach to study contact specific gene regulation during embryogenesis.

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LIM homeobox protein 5 (Lhx5) is essential for mamillary body development

Cited by 11 publications

References 61 publications

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

Developmental genoarchitectonics as a key tool to interpret the mature anatomy of the chondrichthyan hypothalamus according to the prosomeric model

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development

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