LIM-class homeobox gene Lim1, a novel oncogene in human renal cell carcinoma

Dormoy, Valérian; Beraud, C; Lindner, Véronique; Thomas, Lionel; Coquard, Catherine; Barthelmebs, Mariette; Jacqmin, Didier; Lang, Hervé; Massfelder, Thierry

doi:10.1038/onc.2010.557

Cited by 31 publications

(30 citation statements)

References 37 publications

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“…A number of studies have reported LIM1 expression in human cancer cells including leukemic cells, renal carcinoma and ovarian cancer epithelial cells (Dong et al 1997;Bowen et al 2009;Dormoy et al 2010). We also detected LIM1 expression in a number of uterine epithelial cancer cell lines and mRNA of primary cancer samples (data not shown).…”

Section: Discussionsupporting

confidence: 45%

Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

Evans

Gargett

2012

Histochem Cell Biol

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Lim1 encodes a homeodomain transcription factor required for head, kidney and female reproductive tract development in the murine embryo. Recently, Lim1 expression was documented in several adult murine and human organs. In the developing female reproductive tract, Lim1 expression was first detected in the Müllerian ducts. Using immunofluorescence, we detected LIM1 expression in a developmental model of human female reproductive tract which was established by recombination of neonatal uterine mesenchyme with human embryonic stem cells. In addition, we report a dynamic expression of Lim1/LIM1 in neonatal and adult mouse, and adult human endometrial epithelium and stroma as revealed by immunofluorescence and quantitative real-time polymerase chain reaction. LIM1 expression was also observed in several endometrial epithelial cancer cell lines (ECC-1, Ishikawa, and HEC1A). Furthermore, we found that Activin A significantly upregulated LIM1 mRNA expression in ECC-1 cells. These studies demonstrate previously unreported Lim1/LIM1 expression in neonatal, adult mouse and human endometrium suggesting Lim1/LIM1 may have a role in endometrial development and remodelling.

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Section: Discussionsupporting

confidence: 45%

Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

Evans

Gargett

2012

Histochem Cell Biol

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“…In Vivo Ready CDK11 siRNA and non-specific siRNA were purchased from Applied Biosystems. These In Vivo Ready validated CDK11 siRNA were designed using specific algorithms and incorporated with additional chemical modifications for superior serum stability with in vivo applications (17, 19, 31, 32). The Crl:SHO- Prkdc SCID Hr h r female nude mice at 3 to 4 weeks of age were purchased from Charles River Laboratories.…”

Section: Methodsmentioning

confidence: 99%

Cyclin-Dependent Kinase 11 (CDK11) Is Required for Ovarian Cancer Cell Growth In Vitro and In Vivo, and Its Inhibition Causes Apoptosis and Sensitizes Cells to Paclitaxel

Liu

Gao

Shen

et al. 2016

Molecular Cancer Therapeutics

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Ovarian cancer is currently the most lethal gynecological malignancy with limited treatment options. Improved targeted therapies are needed to combat ovarian cancer. Here, we report the identification of cyclin-dependent kinase 11 (CDK11) as a mediator of tumor cell growth and proliferation in ovarian cancer cells. Although CDK11 has not been implicated previously in this disease, we have found that its expression is upregulated in human ovarian cancer tissues and associated with malignant progression. Metastatic and recurrent tumors have significantly higher CDK11 expression when compared with the matched, original primary tumors. RNAi-mediated CDK11silencing by synthetic siRNA or lentiviral shRNA decreased cell proliferation and induced apoptosis in ovarian cancer cells. Moreover, CDK11 knockdown enhances the cytotoxic effect of paclitaxel to inhibit cell growth in ovarian cancer cells. Systemic in vivo administration of CDK11 siRNA reduced the tumor growth in an ovarian cancer xenograft model. Our findings suggest that CDK11 may be a promising therapeutic target for the treatment of ovarian cancer patients.

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“…The sequence of the human TFAP2B-specific siRNA was 5′-GGA CCA GUC UGU CAU UAA ATT-3′ and 5′-CCC GAA AGA AUA UGC UGU UTT-3′, and the scramble siRNA was 5′-UUC UCC GAA CGU GUC ACG UTT-3′. The siRNA and plasmid were incorporated with additional chemical modifications for superior serum stability in the in vivo applications, and the knockdown efficiency was validated in vitro [28,29]. For the in vivo delivery of the siRNA and plasmid into tumors, the sequences were first encapsulated into DOTAP-cholesterol (DC) (Avanti Polar, Birmingham, AL, USA) nanoparticles that had validated by many analyses by dissolving in sterilized de-ionized water and then mixing with the nanosomes.…”

Section: Methodsmentioning

confidence: 99%

TFAP2B overexpression contributes to tumor growth and a poor prognosis of human lung adenocarcinoma through modulation of ERK and VEGF/PEDF signaling

Shi

Wang

et al. 2014

Mol Cancer

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BackgroundTFAP2B is a member of the AP2 transcription factor family, which orchestrates a variety of cell processes. However, the roles of TFAP2B in regulating carcinogenesis remain largely unknown. Here, we investigated the regulatory effects of TFAP2B on lung adenocarcinomas growth and identified the underlying mechanisms of actions in non-small cell lung cancer (NSCLC) cells.MethodsWe first examined the expression of TFAP2B in lung cancer cell lines and tumor tissues. We also analyzed the prognostic predicting value of TFAP2B in lung adenocarcinomas. Then we investigated the molecular mechanisms by which TFAP2B knockdown or overexpression regulated lung cancer cell growth, angiogenesis and apoptosis, and further confirmed the role of TFAP2B in tumor growth in a lung cancer xenograft mouse model.ResultsTFAP2B was highly expressed in NSCLC cell lines and tumor tissues. Strong TFAP2B expression showed a positive correlation with the poor prognoses of patients with lung adenocarcinomas (P < 0.001). TFAP2B knockdown by siRNA significantly inhibited cell growth and induced apoptosis in NSCLC cells in vitro and in a lung cancer subcutaneous xenograft model, whereas TFAP2B overexpression promoted cell growth. The observed regulation of cell growth was accompanied by the TFAP2B-mediated modulation of the ERK/p38, caspase/cytochrome-c and VEGF/PEDF-dependent signaling pathways in NSCLC cells.ConclusionsThese results indicate that TFAP2B plays a critical role in regulating lung adenocarcinomas growth and could serve as a promising therapeutic target for lung cancer treatment.

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LIM-class homeobox gene Lim1, a novel oncogene in human renal cell carcinoma

Cited by 31 publications

References 37 publications

Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

Cyclin-Dependent Kinase 11 (CDK11) Is Required for Ovarian Cancer Cell Growth In Vitro and In Vivo, and Its Inhibition Causes Apoptosis and Sensitizes Cells to Paclitaxel

TFAP2B overexpression contributes to tumor growth and a poor prognosis of human lung adenocarcinoma through modulation of ERK and VEGF/PEDF signaling

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