Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21

Hu, Bing; Du, Qin; Deng, Shan; An, Hong‐Mei; Chuan-fang, Pan; Shen, Ke‐Ping; Xu, Ling; Wei, Mengmeng; Wang, Shuangshuang

doi:10.3892/or.2014.3312

Cited by 36 publications

(32 citation statements)

References 27 publications

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“…Given the findings of the present study, these herbs may increase healthy WBC without aggravating the leukemia. This can be supported by the prior studies of these two herbs demonstrating that they bolster host immune response for cytotoxic activity by promoting apoptosis of tumor cells and may help the recovery from chemotherapy or radiation treatments [18, 24–27]. The findings of the present study provide important information for TCM physicians and patients with leukemia.…”

Section: Discussionsupporting

confidence: 78%

The Effectiveness of Traditional Chinese Medicine in Treating Patients with Leukemia

Wang

Liao

Chen

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Leukemia is the most common malignancy among all childhood cancers and is associated with a low survival rate in adult patients. Since 1995, the National Health Insurance (NHI) program in Taiwan has been offering insurance coverage for Traditional Chinese Medicine (TCM), along with conventional Western medicine (WM). This study analyzes the status of TCM utilization in Taiwan, in both pediatric and adult patients with leukemia. A retrospective cohort study was conducted using population-based National Health Insurance Research Database of Registry of Catastrophic Illness, involving patient data from 2001 to 2010 and follow-up data through 2011. The effectiveness of TCM use was evaluated. Relevant sociodemographic data showed that both pediatric and adult patients who were TCM users one year prior to leukemia diagnosis were more likely to utilize TCM services for cancer therapy. A greater part of medical expenditure of TCM users was lower than that of TCM nonusers, except little discrepancy in drug fee of adult patients. The survival rate is also higher in TCM users. Altogether, these data show that TCM has the potential to serve as an adjuvant therapy when combined with conventional WM in the treatment of patients with leukemia.

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Section: Discussionsupporting

confidence: 78%

The Effectiveness of Traditional Chinese Medicine in Treating Patients with Leukemia

Wang

Liao

Chen

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…Nüzhenzi (Ligustrum lucidum Ait. Fruit), an herb for tonifying liverYin, is potent to up regulate p21, activate caspases8, 9 and 3 to induce apoptosis and downregulate RB phosphorylation to induce cell senescence in hepatocarcinoma cells [154] ( Figure 1). …”

Section: Induction Of Cell Senescencementioning

confidence: 99%

Traditional Chinese medicine for prevention and treatment of hepatocarcinoma: From bench to bedside

Wang

2015

WJH

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and other TCM syndromes (Zheng). Modern treatments such as surgery, transarterial chemoembolization (TACE) and high intensity focus ultrasound treatment would influence the manifestation of TCM syndromes. Herbs with traditional efficacy of tonifying Qi, blood and Yin, soothing liver-Qi stagnation, clearing heat and detoxifying and dissolving stasis, have been demonstrated to be potent to prevent hepatocarcinogenesis. TCM has been widely used in all aspects of integrative therapy in hepatocarcinoma, including surgical resection, liver transplantation, TACE, local ablative therapies and even as monotherapy for middle-advanced stage hepatocarcinoma. Clinical practices have confirmed that TCM is effective to alleviate clinical symptoms, improve quality of life and immune function, prevent recurrence and metastasis, delay tumor progression, and prolong survival time in hepatocarcinoma patients. The effective mechanism of TCM against hepatocarcinoma is related to inducing apoptosis, autophagy, anoikis and cell senescence, arresting cell cycle, regulating immune function, inhibiting metastasis and angiogenesis, reversing drug resistance and enhancing effects of chemotherapy. Along with the progress of research in this field, TCM will contribute more to the prevention and treatment of hepatocarcinoma. AbstractTraditional Chinese medicine (TCM) has played a positive role in the management of hepatocarcinoma. Hepatocarcinoma patients may present Qi-stagnation, damp-heat, blood stasis, Qi-deficiency, Yin-deficiency REVIEWSubmit a

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“…For siRNA transfection, LS174T cells were cultured in six-well plates to 60% confluency, and 80 pmol of specific or control siRNA was introduced into the cells using Lipofectamine™ 2000, according to the manufacturer’s recommendations 8. After 24 h of transfection, the cells were treated with TLBZT and subjected to further assays.…”

Section: Methodsmentioning

confidence: 99%

“…Western blot was performed as described previously 8,9. Briefly, the collected cells were lysed, and total protein was separated by 8%–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane.…”

Section: Methodsmentioning

confidence: 99%

Teng-Long-Bu-Zhong-Tang induces p21-dependent cell senescence in colorectal carcinoma LS174T cells via histone acetylation

Hu¹,

An²,

Wang³

et al. 2017

JEP

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Teng-Long-Bu-Zhong-Tang (TLBZT) is a Chinese herbal formula for colorectal carcinoma treatment. TLBZT effectively induces cell senescence in colorectal carcinoma, accompanied by p21 upregulation. In this study, we further explored the role of p21 in TLBZT-induced cell senescence, as well as the mechanism by which TLBZT upregulates p21. Specific knockdown of p21 expression by small interfering RNA significantly attenuated TLBZT-induced cell senescence in human colorectal carcinoma LS174T cells. Silencing of p53 by small interfering RNA did not affect TLBZT-induced p21 upregulation. Meanwhile, TLBZT inhibited histone deacetylase activity. Furthermore, TLBZT increased acetylation levels of histone H3 and H4, enhancing their binding to the p21 promoter. These data suggested that TLBZT induces cell senescence in LS174T cells through a mechanism involving p21 upregulation via histone H3 and H4 acetylation. This study provides new insights into the application of TLBZT for colorectal carcinoma treatment.

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Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21

Cited by 36 publications

References 27 publications

The Effectiveness of Traditional Chinese Medicine in Treating Patients with Leukemia

The Effectiveness of Traditional Chinese Medicine in Treating Patients with Leukemia

Traditional Chinese medicine for prevention and treatment of hepatocarcinoma: From bench to bedside

Teng-Long-Bu-Zhong-Tang induces p21-dependent cell senescence in colorectal carcinoma LS174T cells via histone acetylation

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