Ligustrazine recovers thiram-induced tibial dyschondroplasia in chickens: Involvement of new molecules modulating integrin beta 3

Mehmood, Khalid; Zhang, Hui; Jiang, Xiong; Yao, Wangyuan; Tong, Xili; Iqbal, Muhammad Kashif; Rehman, Mujeeb Ur; Iqbal, Mujahid; Waqas, Muhammad; Qamar, Hammad; Zhang, Jialü; Li, Jiakui

doi:10.1016/j.ecoenv.2018.10.080

Cited by 32 publications

(27 citation statements)

References 38 publications

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“…Integrins took part in the angiogenesis and considered a potential antiangiogenic target which upregulated in tumor related blood vessels [40,41]. The results of this study are in line with recent studies of Khalid Mehmood et al, [42], who examined the expression of ITGB3 in thiram treated chickens and found downregulation of ITGB3 gene on day 14. Similarly, we have found ITGB3, including ITGAV, ITGA2, ITGB2, and ITGA5 integrin genes downregulated in TD-effected chickens on day 15 as compared to normal chickens.…”

Section: Discussionsupporting

confidence: 90%

“…Similarly, we have found ITGB3, including ITGAV, ITGA2, ITGB2, and ITGA5 integrin genes downregulated in TD-effected chickens on day 15 as compared to normal chickens. Khalid Mehmood et., al [42] has found that, the usage of ligustrazine in TD chickens significantly increased the expression of ITGB3 which helps to prevent the TD. We found five integrin family downregulated genes on day 15 of thiram-induced TD chickens-including, ITGB3 and other integrins such as ITGAV, ITGA2, ITGB2, and ITGA5 may have a crucial role in angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome-based screening of intracellular pathways and angiogenesis related genes at different stages of thiram induced tibial lesions in broiler chickens

et al. 2020

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Background: The Tibial dyschondroplasia (TD) in fast-growing chickens is mainly caused by improper blood circulation. The exact mechanism underlying angiogenesis and vascularization in tibial growth plate of broiler chickens remains unclear. Therefore, this research attempts to study genes involved in the regulation of angiogenesis in chicken red blood cells. Twenty-four broiler chickens were allotted into a control and thiram (Tetramethyl thiuram disulfide) group. Blood samples were collected on day 2, 6 (8-and 14-days old chickens) and 15 (23 days old chickens).Results: Histopathology and hematoxylin and eosin (H&E) results showed that angiogenesis decreased on the 6th day of the experiment but started to recover on the 15th day of the experiment. Immunohistochemistry (IHC) results confirmed the expressions of integrin alpha-v precursor (ITGAV) and clusterin precursor (CLU). Transcriptome sequencing analysis evaluated 293 differentially expressed genes (DEGs), of which 103 up-regulated genes and 190 down-regulated genes were enriched in the pathways of neuroactive ligand receptor interaction, mitogen-activated protein kinase (MAPK), ribosome, regulation of actin cytoskeleton, focal adhesion, natural killer cell mediated cytotoxicity and the notch signalling pathways. DEGs (n = 20) related to angiogenesis of chicken erythrocytes in the enriched pathways were thromboxane A2 receptor (TBXA2R), interleukin-1 receptor type 1 precursor (IL1R1), ribosomal protein L17 (RPL17), integrin beta-3 precursor (ITGB3), ITGAV, integrin beta-2 precursor (ITGB2), ras-related C3 botulinum toxin substrate 2 (RAC2), integrin alpha-2 (ITGA2), IQ motif containing GTPase activating protein 2 (IQGAP2), ARF GTPase-activating protein (GIT1), proto-oncogene vav (VAV1), integrin alpha-IIb-like (ITGA5), ras-related protein Rap-1b precursor (RAP1B), tyrosine protein kinase Fyn-like (FYN), tyrosine-protein phosphatase non-receptor type 11 (PTPN11), protein patched homolog 1 (PTCH1), nuclear receptor corepressor 2 (NCOR2) and mastermind like protein 3 (MAML3) selected for further confirmation with qPCR. However, commonly DEGs were sarcoplasmic/ endoplasmic reticulum calcium ATPase 3 (ATP2A3), ubiquitin-conjugating enzyme E2 R2 (UBE2R2), centriole cilia and spindle-associated protein (CCSAP), coagulation factor XIII A chain protein (F13A1), shroom 2 isoform X6 (SHROOM2), ras GTPase-activating protein 3 (RASA3) and CLU.Conclusion: We have found potential therapeutic genes concerned to erythrocytes and blood regulation, which regulated the angiogenesis in thiram induced TD chickens. This study also revealed the potential functions of erythrocytes.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Transcriptome-based screening of intracellular pathways and angiogenesis related genes at different stages of thiram induced tibial lesions in broiler chickens

et al. 2020

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“…Thiram is used in experimental birds for the induction of TD to explore the fundamental pathway of TD. However, liver metabolic activities are disturbed by the poisonous effects of thiram (Shahzad et al, 2014b;Mehmood et al, 2019a). Chicken tibial cartilage is developed by a series of processes and there is involvement of various key enzymes which plays a pivotal role in cartilage development.…”

Section: Discussionmentioning

confidence: 99%

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Qamar¹

2019

PVJ

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Globally Tibial Dyschondroplasia (TD) is a serious metabolic syndrome in broilers that is identified by the accretion of the growth plate (GP) into the tibialmetaphyseal portion. Grape seed extract (GSE) is a well-known significant antioxidant since it possesses multifaceted anti-inflammatory, anti-cancer and reducing reactive oxygen ability. The experiment was intended to evaluate the proficiency of GSE to recover the chicks affected with TD. For this purpose, 210 chicks were bought and fed normal starter feed for the first 3 days. After that two groups were made having 70 (Control group; normal feed) and 140 chickens (Thiram group; normal feed+ thiram @ 50 mg/kg). Further two equal divisions were made in Thiram group on 7 th day viz TD group and GSE group (@ 50 mg/kg/day). GSE significantly (P<0.05) reverted the blood serum profile and liver antioxidants metabolites and, reduced the histopathological abrasion of the liver as compared to the TD group. Furthermore, GSE chicks exhibited a significant decrease (P<0.05) in CA2 gene expression and less mortality, TD score and GP size. In inference, GSE is a natural plant-based extract which owns solid scavenging and bone healing property, and strong antioxidant capacity. GSE provided recovery to chicks affected with TD chicks via dropping TD score, whereas stabilized the GP width through the CA2 mRNA downregulation. ©2019 PVJ. All rights reservedKey words: Broiler Carbonic anhydrase Liver oxidative stress Tibial dyschondroplasia lesion Toxicity To Cite This Article: Qamar H, Mehmood K, Li A, Waqas M, Iqbal M, Zhang L, Tong X and Li J, 2019. Recovery of chickens affected with tibial dyschondroplasia by application of grape seed extract through downregulating ca2 gene and enhancing liver functions. Pak Vet J, 39(4): 527-533. http://dx.

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“…A 4 µm thick incision was made to prepare the slides. These paraffin-embedded segments were dewaxed in xylene and stained with H&E stain [47]. A light microscope was used to observe the pathological changes in the tibial bones.…”

Section: Hematoxylin and Eosin (Hande) Staining And Immunohistochemistrymentioning

confidence: 99%

“…Reaction conditions used for RT-PCR were the same as reported in our previous experiment [49]. Reference gene GAPDH was used to determine relative expression of target genes through RT-PCR using∆∆CT method [47].…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling

Qamar

Waqas

et al. 2019

Biomolecules

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Tibial dyschondroplasia (TD) negatively affects broilers all over the world, in which the accretion of the growth plate (GP) develops into tibial proximal metaphysis. Plastrum testudinis extract (PTE) is renowned as a powerful antioxidant, anti-inflammatory, and bone healing agent. The current study was conducted to evaluate the efficacy of PTE for the treatment of thiram-induced TD chickens. Broilers (day old; n = 300) were raised for 3 days with normal feed. On the 4th day, three groups (n = 100 each) were sorted, namely, the control (normal diet), TD, and PTE groups (normal diet+ thiram 50 mg/kg). On the 7th day, thiram was stopped in the TD and PTE group, and the PTE group received a normal diet and PTE (30 mg/kg/day). Plastrum testudinis extract significantly restored (p < 0.05) the liver antioxidant enzymes, inflammatory cytokines, serum biochemicals, GP width, and tibia weight as compared to the TD group. The PTE administration significantly increased (p < 0.05) growth performance, vascularization, AKT (serine/threonine-protein kinase), and PI3K expressions and the number of hepatocytes and chondrocytes with intact nuclei were enhanced. In conclusion, PTE has the potential to heal TD lesions and act as an antioxidant and anti-inflammatory drug in chickens exposed to thiram via the upregulation of AKT and PI3K expressions.

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Ligustrazine recovers thiram-induced tibial dyschondroplasia in chickens: Involvement of new molecules modulating integrin beta 3

Cited by 32 publications

References 38 publications

Transcriptome-based screening of intracellular pathways and angiogenesis related genes at different stages of thiram induced tibial lesions in broiler chickens

Transcriptome-based screening of intracellular pathways and angiogenesis related genes at different stages of thiram induced tibial lesions in broiler chickens

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling

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