Ligustilide prevents LPS-induced iNOS expression in RAW 264.7 macrophages by preventing ROS production and down-regulating the MAPK, NF-κB and AP-1 signaling pathways

Y, Su; Chiou, Wen-Fei; Chao, Shiou-Huei; Lee, Meng-Hwan; Chen, Chien-Chih; Tsai, Ying-Chieh

doi:10.1016/j.intimp.2011.03.014

Cited by 131 publications

(77 citation statements)

References 29 publications

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“…In addition, we demonstrated that NF-κB and JNK contribute to the cytotoxicity induced by bafilomycin A1 or concanamycin A (Hong et al, 2006). With regard to the relationship between NO production and ROS production, Su et al (2011) suggested that ROS induce NO production in LPS-stimulated RAW 264 cells, and we reported previously that NO produced by treatment with bafilomycin A1 or concanamycin A plays a role in the cytotoxicity induced by these V-ATPase inhibitors (Hong et al, 2006). Therefore, the present findings suggest that concomitantly produced ROS and NO might comediate the cytotoxic effects of bafilomycin A1 or concanamycin A.…”

Section: Resultsmentioning

confidence: 84%

Increased production of reactive oxygen species by the vacuolar-type (H<sup>+</sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

Yokomakura

Hong²,

Ohuchi³

et al. 2012

J. Toxicol. Sci.

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-Treatment of the mouse leukemic cell line RAW 264 with bafilomycin A1 or concanamycin A, inhibitors of vacuolar-type (H + )-ATPases (V-ATPases), significantly increased the production of reactive oxygen species (ROS) and decreased cell viability. These effects were significantly suppressed by the presence of N-acetyl cysteine (NAC), an ROS scavenger. si-RNA mediated knockdown of the gene for the c subunit of the V0 domain of V-ATPase also resulted in an increase in ROS production and a decrease in cell viability. These results suggest that decreased cellular V-ATPase activity decreases cell viability by increasing ROS production in RAW 264 cells.

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Section: Resultsmentioning

confidence: 84%

Increased production of reactive oxygen species by the vacuolar-type (H<sup>+</sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

Yokomakura

Hong²,

Ohuchi³

et al. 2012

J. Toxicol. Sci.

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“…Increased ROS generation can damage biological molecules and lead to cells or tissue injury (Rezaie et al 2007, Ziegler et al 2011 as well as induces inflammation by increasing the level of cytokines such as IL-1β, IL-6, and TNF-α (Geronikaki and Gavalas 2006). Furthermore, antioxidant polyphenol compounds inhibit nuclear facor-κB (NF-κB) activation and block the expression of NF-κB-dependent cytokines, iNOS, and COX-2 genes (Ahn et al 2005, Su et al 2011. Based on these studies, ROS and NO generation as well as cell death were assessed in LPS-induced inflammation zebrafish model.…”

Section: A C Bmentioning

confidence: 99%

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Kim¹,

Kim²,

Kang³

et al. 2014

ALGAE

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Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. Polyphenols from E. cava have strong anti-inflammatory activity. However, a large number of the by-products from E. cava processing are discarded. In the present study, to utilize these by-products, we assessed the anti-inflammatory activity of the polyphenol-rich fraction (PRF) from E. cava processing by-product (EPB) in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. Four compounds, namely eckol, eckstolonol, dieckol, and phlorofucofuroeckol-A, were isolated and identified from PRF. We found that PRF suppressed the production of nitric oxide (NO), inducible nitric oxide synthase, and cyclooxygenase-2 in the LPS-induced cells. Furthermore, the protective effect of PRF was investigated in vivo in LPS-stimulated inflammation zebrafish model. PRF had a protective effect against LPS-stimulated toxicity in zebrafish embryos. In addition, PRF inhibited LPS-stimulated reactive oxygen species and NO generation. According to the results, PRF isolated from EPB could be used as a beneficial anti-inflammatory agent, instead of discard.Key Words: anti-inflammation; by-product; Ecklonia cava; polyphenol; seaweeds INTRODUCTIONSeaweeds are rich in vitamins, minerals, proteins, polyphenols, and polysaccharides. Therefore, several species of seaweeds are used in cosmetics, food, and pharmaceuticals. Many studies have shown that seaweeds have diverse bioactivities such as antioxidant, anticoagulant, anti-inflammation, antibacterial, and anticancer activities (Heo et al. 2003, Shin et al. 2006, Kim et al. 2007, Erbert et al. 2012, Ko et al. 2012. In particular, Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. E. cava has been identified to contain various natural substances such as carotenoids, fucoidans, and phlorotannins, which have beneficial biological activities including antioxidant, anti-inflammation, anticoagulant, antiviral, and antidiabetic effects (Kang et al. 2010, 2012b, 2013a. Dried forms of E. cava are consumed, but steam heated E. cava products sometimes are manufactured. When steaming the alga, a large amount of by-product is produced and discarded without utilization. Some studies have suggested that fermenting Received April 25, 2014, Accepted June 3, 2014 *Corresponding Author E-mail: youjinj@jejunu.ac.kr Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Preparation of PRF from EPBEPB was kindly provided by Taerim Co., Ltd. (Jeju, Korea) and then was freeze-dried. The dried EPB powder (5 g) was suspended in 500 mL of 80% ethanol and mechanically stirred for 24 h at room temperature. The solution was filtered and the filtrate was concentrated under reduced pressure to give the oily extract. Aft...

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“…We used an LPS-stimulated, murine macrophage cell line, RAW264.7, as a model of inflammation to determine the effects of LOME. The binding of LPS to downstream signaling cascades, including mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) pathways (14), led to the production of inflammatory mediators from macrophages, such as TNF-α, IL-1β, IL-6, PGE 2 and NO. Our results indicated that LOME inhibited NO, PGE 2 and the pro-inflammatory cytokines in LPS-stimulated macrophages through NF-κB signaling, as well as the MAPK, extracellular signal-regulated kinase (ERK)1/2, p38 and c-Jun N-terminal kinase (JNK)1/2 pathways.…”

Section: Introductionmentioning

confidence: 99%

Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages

Park

Kwon

Yuniato³

et al. 2016

International Journal of Molecular Medicine

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Abstract. Lagerstroemia ovalifolia Teijsm. & Binn. has traditionally been used as an herbal medicine and possesses anti-inflammatory properties. However, the mechanisms underlying its anti-inflammatory effects remain poorly understood. For this purpose, we aimed to investigate the effects of methanolic extract of L. ovalifolia (LOME) on nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) production, as well as the underlying molecular mechanisms responsible for these effects, in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We examined the effects of LOME on the production of NO and PGE 2 in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of LOME, we measured the mRNA or protein expression of the pro-inflammatory mediators induced by LOME in the LPS-stimulated RAW264.7 cells. LOME significantly inhibited the production of NO, PGE 2 , interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 cells. Moreover, LOME suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inhibited the phosphorylation of the mitogen-activated protein kinases (MAPKs), with a reduction in the nuclear translocation of nuclear factor (NF)-κB in LPS-stimulated RAW264.7 cells. Taken together, these findings suggest that LOME may exert anti-inflammatory effects in vitro in LPS-stimulated RAW264.7 macrophages and thus, may have potential for use as an adjuvant treatment of inflammatory diseases.

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Ligustilide prevents LPS-induced iNOS expression in RAW 264.7 macrophages by preventing ROS production and down-regulating the MAPK, NF-κB and AP-1 signaling pathways

Cited by 131 publications

References 29 publications

Increased production of reactive oxygen species by the vacuolar-type (H<sup>+</sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

Increased production of reactive oxygen species by the vacuolar-type (H<sup>+</sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Amelioration of an LPS-induced inflammatory response using a methanolic extract of Lagerstroemia ovalifolia to suppress the activation of NF-κB in RAW264.7 macrophages

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