Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1–F3) in late-phase laying hens

Chen, Hao; Chen, Xin; Ping, Zhenlei; Fang, Lixue; Jiang, Xiaowen; Ge, Ming; Ma, Jun; Yu, Wenhui

doi:10.1016/j.psj.2022.102430

Cited by 3 publications

(3 citation statements)

References 62 publications

(70 reference statements)

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“…KDR is mainly expressed in the vascular endothelium of the theca layer in chicken follicles. KDR might be the most important receptor which is involved in VEGF-induced angiogenesis [70][71][72]. FLT1 can regulate the VEGF activity via interacting with VEGF and making it less available to KDR [73].…”

Section: Discussionmentioning

confidence: 99%

Ovarian transcriptome profile from egg-laying period to incubation period of Changshun green-shell laying hens

Chen,

Wen,

2024

Preprint

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Background: Changshun green-shell laying hen with strong broodiness is a Chinese indigenous chicken breed. Little is known about the mechanisms responsible for ovary development of Changshun green-shell laying hens from egg-laying period (LP) to incubation period (BP). Here, RNA sequencing (RNA-seq) of ovaries from Changshun hens in LP and BP was performed to identify candidate genes and pathways associated with broodiness. Results: We identified 1650 differently expressed genes (DEGs), including 429 up-regulated and 1221 down-regulated DEGs, in chicken ovaries between LP and BP groups. GO and KEGG analysis further revealed that these DEGs were mainly involved in the pathways related to follicle development in chicken ovaries, including focal adhesion, MAPK signaling pathway, and FoxO signaling pathway, and vascular smooth muscle contraction, ECM-receptor interaction, and GnRH signaling pathway were down-regulated in incubating ovaries. Eight candidate genes (EGFR, VEGFRKDRL, FLT1, KDR, PDGFRA, TEK, KIT and FGFR3) related to angiogenesis, folliculogenesis, steroidogenesis and oogenesis in ovaries were suggested to play important roles in the ovarian development of Changshun hens during the transition from LP to BP. Conclusions: We discovered critical genes and pathways which is closely associated with ovary development in incubating chickens, indicating the complexity of reproductive behaviour of different chicken breeds.

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Section: Discussionmentioning

confidence: 99%

Ovarian transcriptome profile from egg-laying period to incubation period of Changshun green-shell laying hens

Chen,

Wen,

2024

Preprint

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“…With the continuous growth of tumors, insufficient blood supply leads to hypoxia and necrosis of cells, which in turn induces HIF1α overexpression. HIF1α directly promotes the transcription of VEGF-A, which combines with VEGFR2 to further induce neovascularization (Liu et al 2022a , b ; Chen et al 2023 ). Mielcarska S et al found that the expression of HIF1α and VEGF-A were considerably increased in tumor-free marginal tissues, revealing that non-cancerous cells in the tumor microenvironment may be an important source of VEGF-A expression (Mielcarska et al 2021 ).…”

Section: Regulation Of Vegf-a Expression In Tumorsmentioning

confidence: 99%

Regulation of VEGF-A expression and VEGF-A-targeted therapy in malignant tumors

Kang,

Li,

Liu

et al. 2024

J Cancer Res Clin Oncol

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Vascular endothelial growth factor A (VEGF-A), a highly conserved dimeric glycoprotein, is a key regulatory gene and a marker molecule of angiogenesis. The upregulation of VEGF-A facilitates the process of tumor vascularization, thereby fostering the initiation and progression of malignant neoplasms. Many genes can adjust the angiogenesis of tumors by changing the expression of VEGF-A. In addition, VEGF-A also exhibits immune regulatory properties, which directly or indirectly suppresses the antitumor activity of immune cells. The emergence of VEGF-A-targeted therapy alone or in rational combinations has revolutionized the treatment of various cancers. This review discusses how diverse mechanisms in various tumors regulate VEGF-A expression to promote tumor angiogenesis and the role of VEGF-A in tumor immune microenvironment. The application of drugs targeting VEGF-A in tumor therapy is also summarized including antibody molecule drugs and traditional Chinese medicine.

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“… 10–12 To date, more than 263 bioactive substances have been isolated from different parts of LCH (rhizomes, fibrous roots, and aboveground parts), including volatile oils, organic acids, phenolic acids, phthalates, alkaloids, polysaccharides, ceramides, cerebrosides and terpenoids. 12 Modern pharmacological studies have shown that LCH has analgesic, anti-inflammatory, antioxidant, antitumor, anti-coagulation, anti-depression, anti-aging, anti-atherosclerosis, and improving cardiac function effects. 13–16 However, its potential function and mechanism of action in the treatment of NP remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Ligusticum chuanxiong Hort. Ameliorates Neuropathic Pain by Regulating Microglial M1 Polarization: A Study Based on Network Pharmacology

Cui,

Feng,

Xia

2024

JPR

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Background In traditional Chinese medicine, Ligusticum chuanxiong Hort . (LCH) is used to treat neuropathic pain (NP). This study was performed to investigate the underlying pharmacological mechanisms. Methods The main components of the LCH were obtained from the TCMSP database. The targets of the active components were obtained using the Swiss Target Prediction database and HERB database. The NP-related genes were obtained from the CTD database and GeneCard database. Protein-protein interaction (PPI) network was constructed using the STRING platform and Cytoscape 3.9.0 software. GO and KEGG enrichment analyses were performed using the DAVID database. Interactions between the key components and hub target proteins were verified using molecular docking and molecular dynamics simulation. In addition, microglial cell line HMC3 was induced to polarize to the M1 phenotype using 100 ng/mL lipopolysaccharide (LPS). Quantitative real-time polymerase chain reaction (qRT-PCR), Western blot and enzyme-linked immunosorbent assays were used to detect the expression levels of M1 markers and inflammatory factors, respectively. Results Seven LCH active components of LCH were identified, corresponding to 387 target genes. 2019 NP-related genes were obtained, and a total of 174 NP-related genes were identified as target genes that could be modulated by LCH. Beta-sitosterol, senkyunone, wallichilide, myricanone, and mandenol were considered as the key components of LCH in the treatment of NP. SRC, BCL2, AKT1, HIF1A and HSP90AA1 were identified as the hub target proteins. GO analysis showed that 328 biological processes, 61 cell components, and 85 molecular functions were likely modulated by the components of LCH, and KEGG enrichment analysis showed that 132 signaling pathways were likely modulated by the components of LCH. Beta-sitosterol, senkyunone, wallichilide, myricanone, and mandenol showed good binding activity with hub target proteins including SRC, BCL2, AKT1, and HSP90AA1. In addition, beta-sitosterol inhibited LPS-induced M1 polarization in HMC3 in vitro. Conclusion This study provides a theoretical basis for the application of LCH in the treatment of NP through multicomponent, multitarget, and multiple pathways.

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Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1–F3) in late-phase laying hens

Cited by 3 publications

References 62 publications

Ovarian transcriptome profile from egg-laying period to incubation period of Changshun green-shell laying hens

Ovarian transcriptome profile from egg-laying period to incubation period of Changshun green-shell laying hens

Regulation of VEGF-A expression and VEGF-A-targeted therapy in malignant tumors

Ligusticum chuanxiong Hort. Ameliorates Neuropathic Pain by Regulating Microglial M1 Polarization: A Study Based on Network Pharmacology

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