Lignocellulosic Biomass Utilization Toward Biorefinery Using Meshophilic Clostridial Species

Tamaru, Yutaka; López-Contreras, Ana M.

doi:10.5772/56480

Cited by 2 publications

(2 citation statements)

References 58 publications

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“…CAZymes can break down, create and rearrange oligo-and polysaccharides and play an important role in bacteria and are vital for optimizing biomass degradation [35]. The degradation capacity of strain B5 during the composting process was revealed through the gene annotation against the CAZyme database.…”

Section: Cazyme Family Analysis Of the Genome Of Strain B5mentioning

confidence: 99%

Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation

et al. 2020

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A lignocellulose-degrading strain isolated from thermophilic compost was identified as Geobacillus stearothermophilus B5, and found able to secrete considerable amounts of enzymes at optimal temperature (60 °C) and pH (7.5). One circular contig of 3.37 Mbp was assembled from raw data, and 3371 protein-coding genes were predicted. Clusters of orthologous groups (COG) analysis revealed various genes with functions in polymeric substrate degradation, especially for Carbohydrate Active enZymes (CAZymes), such as glycoside hydrolases (GHs) and glycosyl transferases (GTs). Furthermore, the transcriptional responses of B5 at different temperatures—with rice straw provided as the sole carbon source—were analyzed. The results revealed that B5 could resist high temperature by upregulating heat shock proteins (HSPs), enhancing protein synthesis, and decreasing carbon catabolism. Briefly, B5 possesses the ability of lignocellulose degradation, and might be considered a potential inoculant for improving composting efficiency.

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Section: Cazyme Family Analysis Of the Genome Of Strain B5mentioning

confidence: 99%

Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation

et al. 2020

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show abstract

“…CAZymes can cleave, build and rearrange oligo-and polysaccharides, which play important roles in bacteria and are vital for optimizing biomass degradation [27].The degradation capacity of B5 strain during the composting process was revealed through the gene annotation against the CAZy database. B5 strain encoded 100 CAZymes which unevenly distributed between glycoside hydrolases (GH, 29.0%), glycosyl transferases (GT, 36.0%), carbohydrate esterases (CE, 20.0%), auxiliary activities (AA, 4.0%) and carbohydrate-binding modules (CBM, 11.0%) (Fig.…”

Section: Cazyme Family Analysis Of B5 Strain Genomementioning

confidence: 99%

Genomic and transcriptome analyses of a thermophilic bacterium Geobacillus stearothermophilus B5 isolated from compost reveal its enzymatic basis for lignocellulose degradation

Wang

Miao

Wang

et al. 2020

Preprint

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Background Composting is a special global carbon cycle which sustains various microbes engendering the cellulose degradation. Studies have obtained substantial compost microbiomes, yet the expressions and functions of these lignocellulolytic enzymes remains obscure. Thus, the discovery of thermophilic microorganisms as considerable biochemical catalysts for biofuels is becoming more and more attractive.Results A lignocellulose degrading strain isolated from thermophilic compost was identified as Geobacillus stearothermophilus B5, which could secrete considerable enzymes at the optimal temperature (60°C) and pH (7.5). One single contig of 3.37 Mbp was obtained from raw data and 3371 protein-coding genes were predicted, and the clusters of orthologous groups (COG) analysis revealed various genes with function of polymeric substrates degradation, especially for abundant CAZymes including glycoside hydrolases (GH, 29%) and glycosyl transferases (GT, 36%). Furthermore, the transcriptional responses of B5 at different temperature by using rice straw as sole carbon sources were also analyzed, based on which the mechanism of lignocellulose degradation at high temperature was revealed that B5 could resist the heat by up-regulating the heat shock proteins (HSP) and then secrete various Carbohydrate-Active enzymes (CAZymes) to realize energy balance.Conclusions The comparative whole-genome along with transcriptome analysis indicated that G. stearothermophilus B5 owned the ability of lignocellulose degradation and could be considered as a potential inoculant in composting efficiency, thus are also valuable for the lignocellulosic bioenergy industry.

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Lignocellulosic Biomass Utilization Toward Biorefinery Using Meshophilic Clostridial Species

Cited by 2 publications

References 58 publications

Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation

Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation

Genomic and transcriptome analyses of a thermophilic bacterium Geobacillus stearothermophilus B5 isolated from compost reveal its enzymatic basis for lignocellulose degradation

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