Lignocellulosic biomass as a carbohydrate source for lipid production by Mortierella isabellina

Zeng, Jijiao; Zheng, Yubin; Yu, Xiaochen; Yu, Liang; Gao, Difeng; Chen, Shulin

doi:10.1016/j.biortech.2012.10.079

Cited by 81 publications

(86 citation statements)

References 22 publications

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“…e and Raudonien . e, 2008;Zeng et al, 2013). A third genus identified, Cladosporium, is commonly found on both living and dead plant matter, and a xylanse from Cladosporium cladosporioides was isolated and characterized (Hong et al, 2011).…”

Section: Harvesting Impacts On Hemicellulolytic Microbes Htc Leung Et Almentioning

confidence: 99%

Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils

et al. 2015

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Forest ecosystems need to be sustainably managed, as they are major reservoirs of biodiversity, provide important economic resources and modulate global climate. We have a poor knowledge of populations responsible for key biomass degradation processes in forest soils and the effects of forest harvesting on these populations. Here, we investigated the effects of three timber-harvesting methods, varying in the degree of organic matter removal, on putatively hemicellulolytic bacterial and fungal populations 10 or more years after harvesting and replanting. We used stable-isotope probing to identify populations that incorporated 13C from labeled hemicellulose, analyzing 13C-enriched phospholipid fatty acids, bacterial 16 S rRNA genes and fungal ITS regions. In soil microcosms, we identified 104 bacterial and 52 fungal hemicellulolytic operational taxonomic units (OTUs). Several of these OTUs are affiliated with taxa not previously reported to degrade hemicellulose, including the bacterial genera Methylibium, Pelomonas and Rhodoferax, and the fungal genera Cladosporium, Pseudeurotiaceae, Capronia, Xenopolyscytalum and Venturia. The effect of harvesting on hemicellulolytic populations was evaluated based on in situ bacterial and fungal OTUs. Harvesting treatments had significant but modest long-term effects on relative abundances of hemicellulolytic populations, which differed in strength between two ecozones and between soil layers. For soils incubated in microcosms, prior harvesting treatments did not affect the rate of incorporation of hemicellulose carbon into microbial biomass. In six ecozones across North America, distributions of the bacterial hemicellulolytic OTUs were similar, whereas distributions of fungal ones differed. Our work demonstrates that diverse taxa in soil are hemicellulolytic, many of which are differentially affected by the impact of harvesting on environmental conditions. However, the hemicellulolytic capacity of soil communities appears resilient.

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Section: Harvesting Impacts On Hemicellulolytic Microbes Htc Leung Et Almentioning

confidence: 99%

Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils

et al. 2015

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“…[20][21][22][23][24] Some yeast strains present high cell density and lipid yield on lignocellulosic sugars, such as Lipomyces starkeyi, Rhodosporidium toruloides and Trichosporon fermentans, which are able to utilize glucose as the carbon source to accumulate 56%, 67.5% and 62% intracellular lipids, respectively. 20,[30][31][32][33] Mortierella isabellina is a filamentous fungus that has shown such capabilities. 23,24,28,29 Compared to oleaginous yeasts, oleaginous fungi demonstrate the capabilities to effectively overcome these technical issues and still maintain comparable lipid accumulation on lignocellulosic hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

A sustainable lignocellulosic biodiesel production integrating solar- and bio-power generation

et al. 2016

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A solar-bio-powered refining concept has been developed to establish sustainable lignocellulosic biodiesel production. The refining concept applies a combined hydrolysis process without detoxification to release sugars from corn stover, uses aerobic oleaginous fungal fermentation to accumulate lipids on the hydrolysate for biodiesel production, and implements solar-bio-power generation to satisfy the energy demand. The results demonstrate that system integration of solar-bio-power generation and fungal lipid accumulation is an effective approach to create a sustainable lignocellulosic biodiesel production. Compared to concentrated solar power (CSP) units, photovoltaics (PV) are a more suitable solar technology to satisfy the energy needs of the lignocellulosic biodiesel production. The PV-bio-powered lignocellulosic biodiesel production has a net energy output of 28.70 MJ kg −1 biodiesel produced, and a net energy ratio (NER) of 1.08. † Electronic supplementary information (ESI) available. See

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“…Phacidium lacerum , Exophiala calicioides , and Geomyces destructans are all identified as plant root pathogens (Table 2) [27]. Most of RFLP types species belonging to Mucoromycotina were related to Mortierella , which is extensively studied as single-cell oil production fungi using lignocellulosic sugars [29]. Most of identified Basidiomycota clones belong to the wood rotting fungi, including Clitopilus prunulus (1 RFLP types, 2 clones), Trechispora alnicola (1 RFLP type, 2 clones), Rhizoctonia sp.…”

Section: Resultsmentioning

confidence: 99%

Community Structure and Succession Regulation of Fungal Consortia in the Lignocellulose-Degrading Process on Natural Biomass

Tian

Wang

et al. 2014

The Scientific World Journal

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The study aims to investigate fungal community structures and dynamic changes in forest soil lignocellulose-degrading process. rRNA gene clone libraries for the samples collected in different stages of lignocellulose degradation process were constructed and analyzed. A total of 26 representative RFLP types were obtained from original soil clone library, including Mucoromycotina (29.5%), unclassified Zygomycetes (33.5%), Ascomycota (32.4%), and Basidiomycota (4.6%). When soil accumulated with natural lignocellulose, 16 RFLP types were identified from 8-day clone library, including Basidiomycota (62.5%), Ascomycota (36.1%), and Fungi incertae sedis (1.4%). After enrichment for 15 days, identified 11 RFLP types were placed in 3 fungal groups: Basidiomycota (86.9%), Ascomycota (11.5%), and Fungi incertae sedis (1.6%). The results showed richer, more diversity and abundance fungal groups in original forest soil. With the degradation of lignocellulose, fungal groups Mucoromycotina and Ascomycota decreased gradually, and wood-rotting fungi Basidiomycota increased and replaced the opportunist fungi to become predominant group. Most of the fungal clones identified in sample were related to the reported lignocellulose-decomposing strains. Understanding of the microbial community structure and dynamic change during natural lignocellulose-degrading process will provide us with an idea and a basis to construct available commercial lignocellulosic enzymes or microbial complex.

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Lignocellulosic biomass as a carbohydrate source for lipid production by Mortierella isabellina

Cited by 81 publications

References 22 publications

Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils

Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils

A sustainable lignocellulosic biodiesel production integrating solar- and bio-power generation

Community Structure and Succession Regulation of Fungal Consortia in the Lignocellulose-Degrading Process on Natural Biomass

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