Lignocellulosic biomass: Biosynthesis, degradation, and industrial utilization

Guerriero, Gea; Hausman, Jean-François; Strauss, Joseph; Ertan, Haluk; Siddiqui, Khawar Sohail

doi:10.1002/elsc.201400196

Cited by 170 publications

(118 citation statements)

References 126 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…The rate of the enzymatic reaction depends on a number of factors, particularly pH and temperature. Achievement of high activity of the enzyme is possible in a limited pH range, which ensures proper conditions for the reaction related to the presence and distribution of the electric charge in the active center and the rest of the protein (Guerriero et al 2016). In contrast, the inactivation of the biocatalyst by changing the acidity of the environment or increasing the temperature causes loss of catalytic activity.…”

Section: The Characteristics Of the Biopreparationmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

View full text Add to dashboard Cite

A multi-enzymatic biopreparation of Trichoderma atroviride G79/11 origin was characterized. The fungus showed relatively high cellulase production in a soybean flour-cellulose-lactose medium. Subsequently, based on its post-culture liquid, the biopreparation of the enzyme mixture was developed and characterized. The liquid form of the enzyme mixture reached 22 U cm -3 of cellulolytic activity and its lyophilisate exhibited 1.09 U cm -3 at pH 5.1 and 50 °C. The enzyme mixture was characterized by the following activities: xylanase, β-glucosidase, carboxymethyl cellulase, polygalactouronase, pectinesterase, amylase, lactase, and protease. A method for an efficient conditioning process of organic waste (fruit processing waste, dairy sewage sludge, corn silage, and grain broth) for biogas yield enhancement using the enzyme mixture was proposed. The enzyme mixture increased the efficacy of biogas production by 30% when the lyophilizate (0.5 mg g -1 d.m.) was applied prior to fermentation. A method for conducting the enzymatic conditioning process of organic waste using the enzyme mixture as a pretreatment was proposed. This was part of the optimization of the methane fermentation process to increase the biogas yield. Consequently, after application of the biopreparation, the efficiency of anaerobic digestion of organic waste was improved.

show abstract

Section: The Characteristics Of the Biopreparationmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

View full text Add to dashboard Cite

show abstract

“…In contrast, the maximum liberation of reducing sugars from SB and WB by recombinant strains was up to 2-fold higher than obtained in those reports. The increase in fermentable sugars from the third day of incubation employing recombinant P. chrysosporium strains could be a preliminary step to reduce the time of saccharification and possibly the costs to the additional use of commercial hydrolytic enzymes for biological methods [Guerriero et al, 2016]. Nevertheless, a complete analysis should be performed to define the optimal conditions of saccharification and to determine which enzymes are involved in the hydrolysis of these sugars.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus Phanerochaete chrysosporium Overexpressing Laccases and Peroxidases

et al. 2018

View full text Add to dashboard Cite

Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.

show abstract

“…Cellulose, hemicellulose and lignin are the three largest components of lignocellulosic biomass [5]. Cellulose, the largest component in most of botanic body, covers 35%~50% of plant dry weight.…”

Section: Composition Of Agricultural Crop Wastesmentioning

confidence: 99%

Pretreatment of Agricultural Crop Wastes for Biofuels Production

Ai¹,

Zheng²,

Zheng³

et al. 2018

Proceedings of the 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017)

View full text Add to dashboard Cite

Abstract.Crop straw is an abundant but still underutilized renewable resource. Most of agricultural crop wastes contain about 70% fermentable components. Pretreatment technology aims to expose the fermentable carbohydrates for hydrolytic enzymes and microbes in downstream biofuels production process. An ideal pretreatment method satisfies following criteria: greatly enhancing the enzymatic efficiency of cellulose, minimizing the loss of fermentable carbohydrates, minimizing the formation of inhibitors, and high cost effectiveness. The most studied pretreatment methods include acid pretreatment, alkaline pretreatment, steam explosion, biological pretreatment, and so on. The effects of pretreatment methods on increasing the biodigestibility include increase of surface areas, reduce of cellulose crystallinity, hemicellulose solubilization, delignification, inhibitors production. Different pretreatment method offers its advantages, and also have its own shortcomings. When selecting a pretreatment method and optimizing operation conditions, not only the characteristics of lignocellulosic feedstock but also the target product and fermentation process need to be considered.

show abstract

Lignocellulosic biomass: Biosynthesis, degradation, and industrial utilization

Cited by 170 publications

References 126 publications

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus <b><i>Phanerochaete chrysosporium</i></b> Overexpressing Laccases and Peroxidases

Pretreatment of Agricultural Crop Wastes for Biofuels Production

Contact Info

Product

Resources

About