Ligninolytic enzymes in <i>Basidiomycetes</i> and their application in xenobiotics degradation

Brazkova, Mariya; Koleva, Ralitza; Galena, Angelova; Yemendzhiev, Husein

doi:10.1051/bioconf/20224502009

Cited by 9 publications

(5 citation statements)

References 166 publications

(262 reference statements)

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“…The white-rot fungi (Basidiomycota) pose a unique lignin-degrading enzymatic complex, which can break down complicated structures, such as extremely resistant to microbial attacks lignin, bisphenols, nonylphenols, synthetic dyes, etc. [10,13,16,17]. Different basidiomycete species are often used as model organisms for implementation in degradation processes.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of pyrene during submerged cultivation of Trametes versicolor

Brazkova,

Angelova,

Stefanova

et al. 2024

BIO Web Conf.

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Pyrene is one of the most persistent pollutants belonging to the group of high molecular weight polycyclic aromatic hydrocarbons. Due to the presence of fused benzene rings these compounds are extremely difficult for biodegradation. The white-rot fungi possess remarkably high potential when it comes to the biodegradation of toxic organic substances with aromatic rings due to their unique lignin-degrading enzymatic complex. In the present study, pyrene with concentrations up to 200 ppm was added to the nutrient medium during submerged cultivation of the higher basidiomycete mushroom Trametes versicolor NBIMCC 8939. The experiment continued for 20 days and samples were taken every 5 days. Analysis of the activities of the enzymes laccase and manganese-dependent peroxidase were performed as well as determination of the residual pyrene concertation and identification of the by-products of the degradation process. It was established that the highest pyrene removal was achieved when the lowest initial concentration was introduced to the medium. The strain T. versicolor NBIMCC 8939 was able to biodegrade 12% of the initially introduced 50 ppm pyrene in the medium.

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Section: Resultsmentioning

confidence: 99%

Biodegradation of pyrene during submerged cultivation of Trametes versicolor

Brazkova,

Angelova,

Stefanova

et al. 2024

BIO Web Conf.

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“…found that Gammaproteobacteria could respond indiscriminately to the exposure to environmental concentrations of soil pollutants, which is an indicator taxon for subsequent assessments of soil ecological risk. Brazkova et al (2022) found that Basidiomycetes produce lignin-degrading enzymes and reduce soil pollution. found that Chaetomium globosum CEF-082 and its metabolic ltrate could not only the growth of the plant but also reduce the incidence of soil-borne disease.…”

Section: Discussionmentioning

confidence: 99%

Effects of Bacillus Safensis YY-01 on the Growth of Tomato

Yang

Zhang

Song

et al. 2022

Preprint

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Purpose Although the use of microbial fertilizers is still far lower than that of chemical fertilizers in the planting industry, it has still received much attention for its non-toxic and pollution-free characteristics. Here a promising bacterial strain was successfully isolated from the tomato rhizosphere and identified as Bacillus Safensis YY-01 with 16S rDNA sequence, which was found to significantly enhance both the growth of tomato and the relative abundance of beneficial microorganisms such as Bacillus, Chaetomium, Proteobacteria, Actinobacteria, and Chloroflexi. B. Safensis YY-01 may be a strong candidate as one bacterial strain of microbial fertilizers. Methods B. Safensis YY-01 was isolated and cultured, which was used as leaf fertilizer to spray on the phylloplane of tomato every week. The effect of B. Safensis YY-01on the height, chlorophyll, and peroxidase (POD) activity of tomato was investigated, moreover, the microbial community structure of tomato rhizosphere soil was analyzed through high-throughput sequencing technology. Results Compared with the control, the height, chlorophyll A, chlorophyll B, total chlorophyll, and POD activity of tomato Haoliang No.998 were increased by 12.8%, 17.1%, 11.8%, 14.8%, and 37.5%, respectively, and yingfen No.8 were increased by 14.1%, 7.8%, 10.2%, 10.7%, and 7.3%, respectively. Further, the microbial community structure in rhizosphere soil of tomato Haoliang No.998 was analyzed and showed that the relative abundance of beneficial microorganisms such as Proteobacteria, Actinobacteria, Chloroflexi, Bacillus, and Gemmatimonadota increased significantly, and the relative abundance of tomato yingfen No.8’s Acidobacteriota, Actinobacteria, and Bacillus were also increased significantly. Conclusion A promising bacterial strain of B. Safensis YY-01 was successfully isolated, which was beneficial to both the growth of tomatoes and the relative abundance of helpful microorganisms in the rhizosphere soil of tomatoes.

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“…In addition, the antifungal activity may be due to more than one metabolite with different effects on the colonies and production times. White rot basidiomycetes (WRB) are a group of fungi that stand out to produce ligninolytic enzymes [46], in addition to a wide variety of structurally diverse bioactive metabolites [47], among which can be found antimycobacterial, antiviral, antifungals, immunomodulators, and anticancer, to name a few [48]. However, the study of the production of these metabolites, their cellular targets, and their application is limited compared to what has been explored for ascomycetes and bacteria.…”

Section: Production Kinetics Of Antifungal Activity In Cell-free Supe...mentioning

confidence: 99%

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

Gutiérrez-Soto,

López-Sandin,

Hernández Ochoa

et al. 2024

Microbiology Research

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Macrolepiota sp. CS185 is a basidiomycete with high potential as a biocontrol agent against various phytopathogenic fungi. Regardless of its pronounced potential as a post-harvest fungi biocontrol agent, its activity in tomato seedlings infected with Alternaria alternata has not been well studied. Thus, the present work aimed to evaluate the cultures and supernatants’ antagonistic activity against fig fruits’ post-harvest fungi and antifungal activity production kinetics. The culture antagonistic characteristics were assessed through multiple confrontations, the supernatant concentration effect, and the kinetics of antagonistic action. The multiple confrontations showed differences (p ≤ 0.05) among phytopathogens and over time, with Colletotrichum sp. 2 being the most susceptible. Based on the 9-day incubation profile, the treatment fractions supplemented with a 50% concentration of Macrolepiota sp. CS185 supernatants showed a higher inhibition percentage (%In). Except for Alternaria alternata 1 and 2, the rest of the isolates showed a similar decrease in antagonistic activity up to a certain extent over time. Among all tested strains, Colletotrichum sp. 2 was found with a higher susceptibility. Regarding the production kinetics of antagonistic activity, a triple interaction was observed between the phytopathogen, the age of the Macrolepiota sp. CS 185 culture, and incubation time. In addition, changes in the mycelium growth rate (p ≤ 0.05) along with the higher activity in the supernatants of 20 and 30 days were observed and suggested the production of multiple bioactive metabolites. These results indicate that Macrolepiota sp. CS185 produces antifungal metabolites at different times and could be a suitable candidate to control fig fruits’ post-harvest fungi issues.

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Ligninolytic enzymes in Basidiomycetes and their application in xenobiotics degradation

Cited by 9 publications

References 166 publications

Biodegradation of pyrene during submerged cultivation of Trametes versicolor

Biodegradation of pyrene during submerged cultivation of Trametes versicolor

Effects of Bacillus Safensis YY-01 on the Growth of Tomato

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

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