Lignocellulosic biomass represents perhaps the most abundant renewable resource with a potential to replace fossilbased feedstock for sustainable energy, chemical and materials production. Among the three chief lignocellulosic biomass components (i.e. cellulose, hemicellulose and lignin), lignin is a macromolecule with an aromatic skeleton with a variety of functional groups (e.g. hydroxyl, methoxy, carbonyl, double bond) and carries a higher energy density. The unique structure makes lignin an intriguing substrate for energy, chemicals and materials productions. However, the high molecular weight and complex macromolecular structure have made lignin a challenging substrate to be transformed by many conversion methods. Microbial enzyme degradation and modification of lignin have been subjected to a significant amount research in the last a few decades. Yet so far little success has been demonstrated to merit the use of enzymatic technology for lignin transformation at a commercial scale. This paper provides an updated review of the development of lignin degrading/modifying enzymes with an emphasis on identifying the key barriers and challenges toward practical applications of microbial enzymes for lignin valorization with a hope to generate new insights and direction that can overcome these challenges. Jou Chin Chan received her B.Sc. in Chemical Engineering in 2014 from Universiti Malaysia Sarawak (Malaysia). She is currently pursuing her Ph.D. at the Voiland School of Chemical and Biological Engineering, Washington State University under the supervision of Professor Xiao Zhang. Her current research includes the understanding of enzymatic lignin depolymerization. Michael Paice has over 40 years of experiences in the Canadian pulp and paper industry and is currently principle of Michael Paice & Associates (MP&A), based in Richmond, BC, providing sustainable environmental solutions for several industrial and government clients.