Ligninolytic enzymes can participate in a multiple response system to oxidative stress in white-rot basidiomycetes: Fomes fomentarius and Tyromyces pubescens

Jaszek, Magdalena; Żuchowski, Jerzy; Dajczak, Elżbieta; Cimek, Kamila; Grąz, Marcin; Grzywnowicz, Krzysztof

doi:10.1016/j.ibiod.2006.06.012

Cited by 36 publications

(40 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These stress factors, which induce oxidative stress, can increase extracellular laccase activity and enhance both superoxide dismutase (SOD) and catalase (CAT) activity. This implies that laccase can participate in the adaptive response to oxidative stress in white rot fungus (33,34).The study of plant-pathogenic fungi has also suggested that laccase is involved in defense against oxidative stress in other fungi besides white rot fungus. One study on the plant-pathogenic fun- As mentioned above, fungal laccase may be involved in the adaptive response to oxidative stress.…”

mentioning

confidence: 97%

mentioning

confidence: 98%

“…It has been observed that oxidative stress can induce the expression of ligninolytic enzymes in some basidiomycetes (6,33,41). The extracellular laccase activity of some white rot basidiomycetes such as Fomes fomentarius, Tyromyces pubescens, Trametes versicolor, and Abortiporus biennis can be stimulated by the oxidative stress caused by exogenous menadione and paraquat.…”

mentioning

confidence: 99%

“…One study on the plant-pathogenic fun- As mentioned above, fungal laccase may be involved in the adaptive response to oxidative stress. However, the hypothesis that laccase plays an important role in defense against oxidative stress is mainly based on the phenomenon that laccase activity can be induced by various oxidative stress factors (2,12,16,20,(32)(33)(34). To our knowledge, there is little direct evidence supporting the involvement of laccase in the defense against oxidative stress.…”

mentioning

confidence: 99%

“…The extracellular laccase activity of some white rot basidiomycetes such as Fomes fomentarius, Tyromyces pubescens, Trametes versicolor, and Abortiporus biennis can be stimulated by the oxidative stress caused by exogenous menadione and paraquat. Enhanced extracellular laccase activity is considered part of the system for the adaptive response of white rot fungus to paraquat-and menadione-caused oxidative stress conditions (33,34). A notable increase in the laccase activity of two fungal species, Trametes versicolor and Abortiporus biennis, can be observed after treatment with oxidative stress factors such as menadione, paraquat, and hydrogen peroxide (12).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H ₂ O ₂ -Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System

Yang

Fan

Zhuo

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTLaccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by which laccase protects cells from oxidative stress also remains unclear. Here, we report that the expression of the laccase gene from white rot fungus inPichia pastoriscan significantly enhance the resistance of yeast to H2O2-mediated oxidative stress. The expression of laccase in yeast was found to confer a strong ability to scavenge intracellular H2O2and to protect cells from lipid oxidative damage. The mechanism by which laccase gene expression increases resistance to oxidative stress was then investigated further. We found that laccase gene expression inPichia pastoriscould increase the level of glutathione-based antioxidative activity, including the intracellular glutathione levels and the enzymatic activity of glutathione peroxidase, glutathione reductase, and γ-glutamylcysteine synthetase. The transcription of the laccase gene inPichia pastoriswas found to be enhanced by the oxidative stress caused by exogenous H2O2. The stimulation of laccase gene expression in response to exogenous H2O2stress further contributed to the transcriptional induction of the genes involved in the glutathione-dependent antioxidative system, includingPpYAP1,PpGPX1,PpPMP20,PpGLR1, andPpGSH1. Taken together, these results suggest that the expression of the laccase gene inPichia pastoriscan enhance the resistance of yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system to protect the cell from oxidative damage.